Detailed procedures for cleaning the sample block
IMPORTANT!
Use these cleaning procedures
only
in conjunction with the complete decontamination
procedure (see “Decontaminate the sample block”
• Rinse the sample block with deionized water.
a.
Pipet a small volume of deionized water into each contaminated well.
b.
In each well, pipet the water up and down several times to rinse the well.
c.
Pipet the water to a waste beaker.
d.
Use a cotton swab to scrub inside of each contaminated well.
e.
Use a lint-free cloth to absorb the excess deionized water.
• Clean the sample block with 95% ethanol.
a.
Pipet a small volume of 95% ethanol solution into each contaminated well.
b.
In each well, pipet the solution up and down several times to rinse the well.
c.
Pipet the ethanol solution to a waste beaker.
IMPORTANT!
Always use deionized water to rinse wells after cleaning with bleach or ethanol
solution.
• Clean the sample block with 10% bleach.
a.
Pipet a small volume of 10% bleach solution into each contaminated well.
b.
In each well, pipet the solution up and down several times to rinse the well.
c.
Pipet the bleach solution to a waste beaker.
IMPORTANT!
Always use deionized water to rinse wells after cleaning with bleach or ethanol
solution.
Chapter 6
Maintain the instrument
Decontaminate the sample block
6
76
QuantStudio
™
1 Plus Real-Time PCR System Installation, Use, and Maintenance Guide