4.
Vortex the plate for 5 seconds, then centrifuge at 750–1,000 ×
g
for 2 minutes.
5.
Confirm that the liquid in each well is at the bottom of the well and free of bubbles. If it is not,
centrifuge the plate again.
IMPORTANT!
Keep the bottom of the plate clean. Fluids and other contaminants on the bottom
of the plate can contaminate the sample block and cause an abnormally high background signal.
Add a custom dye to the instrument
1.
In the instrument home screen, touch
Settings
4
Maintenance and
Service
4
Calibrations
4
Custom
4
Custom Dye
.
2.
Touch
Add Custom Dye
.
3.
Enter the dye information:
Field/option
Action
Custom Dye Name Enter a name for the custom dye.
IMPORTANT!
·
Do not use a system dye name for a custom dye name.
·
Dye names are spacing sensitive and cannot contain special characters.
Type
Select:
•
Reporter
—The dye works in conjunction with a quencher dye to report an
increase of PCR product.
•
Quencher
—The dye suppresses the fluorescence of a reporter dye until
amplification of PCR product.
•
Both
—The dye can be used as a reporter or quencher dye.
4.
Touch
Save
.
IMPORTANT!
You must also add the custom dye to the desktop software dye library before
creating, running, or analyzing experiments that use the custom dye. For detailed information, see
the
QuantStudio
™
Design and Analysis SC Software v1.0 Help
.
Chapter 5
Calibrate and verify instrument performance
Calibrate custom dyes
5
68
QuantStudio
™
1 Plus Real-Time PCR System Installation, Use, and Maintenance Guide
