One Lambda, Inc. | A Thermo Fisher Scientific Brand
Micro SSP Gel System User Manual
TDX-OLI-DMR-PS-0619 Rev. 02
8
10. Once set, remove the Gel Combs by lifting the Gel Comb Holder straight up while holding the Base
(figure 8).
Important:
Waiting too long to remove the Gel Combs can risk damaging the gel.
Figure 8: Removing Gel Combs
Electrophoresis Procedure
1. Refer to the product insert of the kit being used and add the appropriate electrophoresis buffer (1x
Tris Borate EDTA) to fill every well in the gel (figure 9). Only use consumables within their expiration
date. The gel is ready for sample loading.
Important:
Amplified DNA samples should be loaded immediately after buffer is poured.
Ensure all wells are filled and the gel is evenly covered with buffer.
The Gel System should be used by trained professional.
!
CAUTION
: Discard and replace Gel Box when there is any evidence of gel/buffer leakage
observed.
Do not add a greater volume of electrophoresis buffer than stated in kit product insert.
Figure 9: Adding buffer to the wells
2. A standard multichannel pipette or 96 well Transfer Device is recommended for loading samples
(Refer to ‘Additional Accessories-Optional’ section for Transfer Device available for purchase from
One Lambda, Inc.). Make sure all pipette tips are firmly attached and are aligned (Non-autoclaved
tips work best). Load DNA samples into the wells (A through H) (figure 10).
