iSort
™
Automated Cell Sorter User Guide
53
Rinse
The Rinse function removes clogs from the sample and probe lines by back
flushing the lines, and can be used to prevent sample carryover between runs.
Clogs in the system can affect sample flow, stream alignment, and droplet
formation.
Run Rinse
1.
Select
Rinse
from the
Maintenance
screen.
2.
Replace the sample tube in the sample tube holder with an empty tube, then
press
Continue
. The instrument performs the rinse automatically, which takes
approximately 30 seconds to complete.
3.
When completed, press
Done
to return to the Maintenance screen.
Note:
To reduce the likelihood of clog formation when working with cells, do not
exceed the maximum cell density of ~3.0 × 10
7
cells/mL and filter the sample
through a cell strainer before loading it on the instrument (see “Guidelines for
sample preparation”, page 32).
Summary of Contents for invitrogen iSort Series
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