iSort
™
Automated Cell Sorter User Guide
41
Perform
Analysis/Sort
The majority of the steps in the Analysis and Sort workflows are identical. Therefore,
the workflows are described together.
•
If you are running a Sort, start with Step 1.
•
If you are running an Analysis, directly go to Step 4 (page 42).
1.
When prompted, ensure that the collection tubes are placed in the collection
tube holder (page 35), push the collection tray inward until it clicks into place.
2.
Close the sorting compartment door to minimize interfering light and exposure
to aerosols, then press
Continue
.
3.
When prompted, select the collection tube size for Region 1 and Region 2.
Note:
The Sort Tube Size prompt is displayed only if there is a collection tube
adapter for the 1.5-mL and 5-mL tubes in the collection tray. Otherwise, the
instrument assumes that 50-mL collection tubes are being used.
The Sort Tube Size prompt is displayed only for active regions.
Note:
When sorting, the target population defined by Region 1 is sorted into
the collection tube placed in Position 1 (left) in the collection tube holder,
while the target population defined by Region 2 is sorted into the collection
tube placed in Position 2 (right).
Summary of Contents for invitrogen iSort Series
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