ThermoFisher Scientific invitrogen iSort Series, Manual

Page: 125 / 140

iSort
™
Automated Cell Sorter User Guide 125
Fluidics issues
Observation Possible cause Recommended action
System preparation fails
before completing the
checking pressure step.
Sheath pump tubing is worn
out. Replace the Sheath pump tubing.
Error Message: Flow instability
detected. Air bubbles in the system,
worn tubing on the Sheath
peristaltic pump.
The System will attempt another Debubble
cycle on the restart of the workflow.
Check and replace the worn tubing.
Warning Message: Sheath fluid
level low. Low sheath fluid level. Refill the sheath fluid.
Warning Message: Waste Level
High. Waste bottle is nearly full. Empty the contents of the waste bottle.
Warning Message: Sample
Level Low. Low sample level. Pause sort and add buffer, or stop sort.
Automation issues
Observation Possible cause Recommended action
System Preparation is unable
to complete. Liquid droplets in sort output. Clean sort chamber including sort drawer,
door, camera lens, and deflection plates.
Error Message: Automation
failure. Poor flow, sheath level low, air
bubbles. Restart automation after verifying sheath
flow.
Error Message: Found Dirty
lens. Moisture or debris in the
sorting area. Open the sort door and clean the sort
chamber, including the lens.
Error Message: Failed to
Adjust Stream. Moisture or debris in the
sorting area.
Air Bubbles in the system Restart the sort preparation. If the problem
persists, contact Technical Support
(page 139).
Error Message: The
automation software could not
obtain an aligned stable
stream. Clogged nozzle, no sheath
fluid. Verify that the nozzle is not clogged and that
you have sheath flow.
Other issues
Observation Possible cause Recommended action
Event rate drops over time Cells are settling out of
suspension Pause the sort or analysis and mix cells