ThermoFisher Scientific invitrogen iSort Series, Manual

Page: 124 / 140

124 iSort
™
Automated Cell Sorter User Guide
The following tables list a number of issues that may be encountered when operating the iSort ™ Automated
Cell Sorter. Symptoms, possible causes, and recommended actions are provided. If you are unable to
resolve the issue with the information provided or there are any other questions or concerns, contact
Technical Support (page 139).
Error messages and warnings
Error message Possible cause Recommended action
HW Failure Camera Electrical malfunction Reboot system. If problem persists, contact
Technical Support (page 139).
HW Failure Laser Laser has failed and can no
longer communicate. Reboot system. If problem persists, contact
Technical Support (page 139).
HW Failure SACNet Hardware failure Contact Technical Support (page 139).
HW Failure FPGA::Not Found Hardware failure Contact Technical Support (page 139).
HW Failure FPGA ::Load The FPGA software is corrupt or
the board is defective. Contact Technical Support (page 139).
HW Failure
Automation::Timeout
The Automation module did not
report in the required time.
Reboot system. If problem persists, contact
Technical Support (page 139).
HW Failure SacLogger Load
Error
Non-fatal error No action required. If the message persists,
contact Technical Support (page 139).
HW Failure Overcurrent System detected a component
that was drawing excessive
current.
Reboot system. If problem persists, contact
Technical Support (page 139).
HW Failure Stage Motors One of the motor controllers is
not reporting correctly to the
system.
Reboot system. If problem persists, contact
Technical Support (page 139).
Nozzle clogs
Error message Possible cause Recommended action
Nozzle is clogged. Full or partial nozzle clog. Remove nozzle tip, clean and replace tip, or
replace with spare nozzle tip. Nozzle clogs
can be reduced in frequency by ensuring
sample and sheath lines are kept clean as
part of routine maintenance.
Nozzle is not seated properly
in the gimbal. Rotate the nozzle very slightly back and
forth; the nozzle should sit securely in the
gimbal and the alignment pin should be
secure in the nozzle notch.
Automation Failure: The
automation software cannot
detect a stable aligned stream.
Full or partial nozzle clog. Remove nozzle tip, clean and replace tip, or
replace with spare nozzle tip.
No sheath fluid. Fill the sheath fluid container.