Thermo Finnigan SpectraSYSTEM UV6000LP, Reference Manual

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January 2004 Thermo Electron 3-1
3
UV6000LP Quick Start
Introduction
This chapter will help you familiarize yourself in the basic use of the
UV6000LP with ChromQuest. For a more detailed description of the
software and their menus refer to the ChromQuest Ver. 4.1 Software
User’s Guide. This Quick Start chapter describes how to create a
method for analyzing the Thermo Electron standard LC Test Mix
using an HPLC system that includes the UV6000LP photodiode array
detector.
Be sure you have installed your LC instruments according to the
procedures described in their corresponding instrument manuals.
Also, verify that your software is properly installed and that you have
created a system configuration as described in the appropriate
software reference manual.
LC System Setup
In order to perform this quick start procedure, you will need one
ampule of the Thermo Electron LC Test Mix, 75% methanol and 25%
water as the mobile phase, and a Hypersil C8 column. If required, you
can substitute other samples, solvents, and columns that are
convenient for you. You will of course have to make appropriate
changes in the method.
The Thermo Electron LC Test Mix contains 6 ppm tartrazine,
100 ppm toluene, 0.5 ppm anthracene, and 2 ppm acetophenone in
70% methanol and 30% water. It is available from Thermo Electron
in packages of five ready-to-use 1 mL ampules (P/N A3469 -010).
Fill an autosampler vial with the test mix and place the vial in the
autosampler tray at position A01.