Chapter |
Operating Instructions
Confidential, limited rights
PN 103261 | Revision D | 23-Oct-2019
User Manual
Page
24
Mount the tweezer onto the connection grove in the central axis. To do this, first select the
‘Place New Grid’ button in the Vitrobot User Interface to put the central axis in the right
position for mounting the tweezer to it.
The tweezers with grid are subsequently lifted into the climate chamber by selecting the ‘Start
Process’ button in the User Interface, or alternatively, use the foot pedal switch. The
environmental and process parameters (i.e. temperature, humidity, number of blots etc) can
now be set as described in:
Starting Up
– Switching on the Vitrobot on page
3.6 Preparation and Lifting of the Coolant Container
Prior to setting the coolant container in the proper position for vitrification it needs to be
pre-cooled in advance.
To do so, the outer ring of the container must be filled with liquid nitrogen. The central cup
can be pre-cooled with liquid nitrogen before filling with ethane or propane. Cooling is a clear
two-step process, to be carried out in a fume-hood; first the peripheral reservoir will attain
liquid nitrogen temperature; in a second stage the central part having a higher heat capacity
will cool down. Vigorous boiling (“Leidenfrost effect”) followed by a “calm” equilibrium
indicates that the metal parts have attained liquid nitrogen temperature.
Summary of Contents for Vitrobot Mk IV
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