Thermo Scientific VF-F20-A, Operating Manual

Page: 106 / 240

6
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Operation
Page 106 Integral Fraction Collector FT (VF-F20-A) Operating Manual
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Verify that the doors of the modules in the Vanquish system are
closed.
•
Make sure that the system is properly equilibrated.
System Equilibration
System equilibration should include the following operations:
•
Purging the pump ( all channels, including those not used for the
application).
•
In Chromeleon, purge the needle wash system (of autosampler and
fraction collector) to fill the needle wash port with the fresh needle
wash liquid. During purging, the needle wash port is flushed
continuously until the fresh needle wash liquid is present.
•
In Chromeleon, purge the flush pump. The flush pump and flush
buffer loop, and all other flush purge lines are sufficiently flushed,
and clean solvent is ready in both flush pump and flush buffer loop.
See
Flush System (

Page 164) .
•
Flushing the entire chromatography system with the starting solvent
to rinse out any solvent from a previous analysis run.
•
In Chromeleon, run a manual needle internal rinse cycle with the
starting solvent to rinse out any other liquids from the internal
surface of needle capillary and needle of the fraction collector.
•
Warming up (or cooling down) all temperature-controlled devices in
the system to the starting temperature. Temperature-controlled
devices can be, for example,

Column compartment and post-column cooler.

Sample compartment thermostatting in the device.

Fraction collector compartment thermostatting in the device.

Flow cell in a fluorescence detector.
•
Turning on the lamp (or lamps) in the UV/VIS detector.
•
Monitoring the pump pressure and pressure ripple and checking
that the pressure is stable and the ripple within reasonable limits for
the application.