Thermo Scientific Vanquish VH-P10-A-02, Operating Manual

Page: 102 / 238

6 • Operation
Page 102 Vanquish Pumps (VH-P10-A-02) Operating Manual
6.9.2 Gradient Delay Volume, Dwell Volume, and Ripple
The gradient delay volume of an HPLC system is defined as the volume
of the entire flow path between the point where the gradient is formed
and the point where the mobile phase enters the column. Thus, the
gradient delay volume of an HPLC system is the volume that the pump
must deliver until a change in solvent composition reaches the column
inlet.
Figure 28: Gradient delay volume of an HPLC system
No. Description
1 Pump with
1a Point where the gradient is formed (purge unit)
2 Autosampler
3 Column
4 Detector
5 Gradient delay volume of the system
The pump contributes to the system gradient delay volume by the dwell
volume. For the dwell volume of the pump, see
section 9.1 Performance
Specifications, page 216 .