4
Tuning and Calibrating
Displaying the FC-43 Mass Spectrum
62
TSQ Quantum XLS Series User Guide
Thermo Scientific
Displaying the FC-43 Mass Spectrum
The first step in tuning and calibrating the mass spectrometer is to display the FC-43 mass
spectrum.
Y
To display the FC-43 mass spectrum
1. Choose
Start > Programs > Thermo Instruments > TSQ > TSQ Tune
to open the EZ
Tune window.
2. Choose a polarity mode. In this example the mass spectrometer is in positive
polarity
mode. If necessary, click the polarity button.
3. Click the
Define Scan
button to display the Define Scan dialog box. See
4. In the Define Scan dialog box, select or enter the following settings:
• Scan type:
Full Scan
• Scan mode:
Q1MS
• Scan range entry mode:
FM/LM
• First mass:
50
• Last mass:
650
• Scan time:
0.500
Table 5.
Typically observed FC-43 peaks in EI, positive polarity mode
m/z
69
m/z
314
m/z
100
m/z
352
m/z
114
m/z
402
m/z
119
m/z
414
m/z
131
m/z
464
m/z
169
m/z
502
m/z
219
m/z
614
m/z
264
Positive
Negative
Note
For EI negative polarity mode, you might have to set the calibration gas flow to
high.
