Thermo Scientific
TSQ Quantum XLS and TSQ Quantum GC User Guide
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4
Tuning and Calibrating
Tune parameters are instrument parameters that affect the intensity of the ion signal.
Calibration parameters are instrument parameters that affect the mass accuracy and resolution
of the mass spectrum. Tuning and calibrating the TSQ Quantum XLS or TSQ Quantum GC
mass spectrometer automatically with TSQ EZ Tune uses perfluorotributylamine (FC-43) as
the tuning and calibration compound. FC-43 resides in a vial inside the mass spectrometer.
Record the ion signal intensity of FC-43 (see
) just after you tune and calibrate the
mass spectrometer. Periodically check the FC-43 ion signal. Re-tune and calibrate the mass
spectrometer if the FC-43 ion signal falls below 50% of the tuned value. Also, re-tune and
calibrate after switching between EI and CI modes or between positive and negative polarity
modes.
lists typical peaks that are observed in the FC-43 mass spectrum in EI, positive
polarity mode.
Contents
•
Displaying the FC-43 Mass Spectrum
•
Running Auto Tune and Calibration
•
Saving the Tune and Calibration Report
Note
You may need to calibrate more often if you operate in H-SRM mode (as opposed
to SRM mode only).
