15
Calibrating the MS
Evaluating the Spray Stability
58
TSQ Altis, Quantis, and Fortis Hardware Manual
Thermo Scientific
Evaluating the Spray Stability
Use the Plot Chromatogram tool to evaluate the stability of the spray.
Before you begin verify:
• The syringe contains the appropriate calibration solution.
• The Tune window has these setting:
1. In the Define Scan pane (
), set the following parameters:
• Scan Type:
SIM (Q1)
• Scan Range (
m/z
):
622 (602 in Negative Mode)
• Source Fragmentation (V):
30
Figure 26.
Define Scan parameters
2. Check the H-ESI probe position is as follows:
• Depth: low to medium
• Front-to-back position: closest to the MS entrance
• Side-to-side position: center (closest to the MS entrance)
3. Place the MS in
On
mode.
Parameter
Value
Current LC Flow
5 μl/min
Syringe pump settings box
5 μL/min flow rate and a
500 μL syringe volume
Ion polarity mode
Positive
Data type
Profile
