Thermo Scientific TSQ 8000, User Manual

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4
Creating a Method
Creating a MS Instrument Method
Thermo Scientific TSQ 8000 Evo User Guide 55
Figure 54. Setting Acquisition-General Scan Information
i. Use the Time (min) column to set the time that the MS begins to acquire the
scan group. Set the time for the first group late enough to allow the solvent to
move through the system, and early enough to ensure the first analyte will be
captured in the segment. Set subsequent segment times so that the start time will
occur between compounds of interest. You may set up different types of scans in
a single group. The scans will occur in descending order.
Your method may allow sequential full scans and SIM scans of a compound. As
an example, in this method, the mass range of 50–300 will be scanned in
0.2 seconds. Immediately after the full scan finishes, the SIM scans begin. The
SIM scans occur sequentially. Two different SIM masses, at 43 and 44, will be
looked at for 0.05 and 0.08 seconds, sequentially. These simultaneous full scan
and SIM scans will begin 2.5 minutes into the GC run. You will get a complete
set of scans every 0.342 seconds. At 5 minutes into the GC run, the scans change
to a set of SRM scans. The first SRM transition is 292.9 to 257.91 with a
collision energy of 20 eV. The second SRM transition is 262.91 to 192.93 with a
collision energy of 32 eV. Both SRM scans have a dwell time of 0.05 seconds.
You will get a complete set of scans every 0.108 seconds.
Note If some of the following columns are not shown in the Scan pane, you can
right-click and display them. You can also reorganize the columns by clicking the
heading of a column and dragging it to the left or right.
Note The faster the MS scans, the more data points across a peak it will
acquire. However, the precision of individual scans may be reduced.