Thermo Scientific thermoscientific Vanquish UHPLC System, Operating Manual

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5 • Installation
Page 66 UHPLC System
Operating Manual
Setting up the System Flow Connections
1. Connect the waste lines (see Connecting the Waste Lines
( } page 70) ).
2. Set up the flow connections for the pump. For details, refer to the
Operating Manual of the pump.
3. Set up the flow connections for the autosampler. For details, refer to
the Operating Manual of the autosampler.
4. Set up the flow connections for the column compartment. For
details, refer to the section Setting Up the Flow Connections in the
Operating Manual of the column compartment. Connect the pre-
heater from port 2 on the autosampler injection valve to the column
outlet tubing using a Viper union. The union replaces the column for
the flushing procedure. Do not install the column yet!
5. Connect a waste tubing to the outlet from the column
compartment, for example, post-column cooler (if applicable) or the
detector inlet capillary (depending on system configuration), and
column switching valve (if applicable) and route it to waste. Do not
install a flow cell yet.
6. Turn on the system (see Turning On the System ( } page 78) ).
7. Set up the system in Chromeleon (see Setting Up the System in the
Software (
}
page 79) ).
8. Flush the system without column (see Flushing the System
( } page 81) ).
9. Install a column. For details, refer to the Operating Manual of the
column compartment. Route the column outlet to waste.
10. Flush the system without flow cell (see Flushing the System
( } page 81) ).
11. Install a flow cell, if applicable. Connect the inlet capillary and waste
line to the detector. For details, refer to the Operating Manual of
your Vanquish detector .
12. Flush the system again with flow cell (if applicable) (see Flushing the
System (
}
page 81) ).
5.5.3 Guiding Capillaries and Tubing Through the System
Flow connections between the modules of the Vanquish system are
guided through either the tubing chase in the devices or the guide holes
or capillary clips of the devices.