Thermo Scientific thermoscientific Vanquish UHPLC System, Operating Manual

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8 • Troubleshooting
Page 120 UHPLC System
Operating Manual
8.2.5 Pressure
Pressure pulsation or inconstant pressure
Possible Cause Remedial Action
Air trapped in the
system Purge the pump (refer to Pump Operating Manual ).
Inlet and/or outlet
check valve
contaminated or
defective Clean the inlet check valve or outlet check valve, and replace the check valve if
necessary (refer to
Pump Operating Manual ).
Pump inline filter (or
static mixer) clogged Check the permeability of the inline filter (or static mixer). Replace the inline filter
(or static mixer) if necessary (refer to
Pump Operating Manual ).
Solvent line filter
clogged Check the solvent line filters for permeability. Replace the filter frits if necessary
(refer to
Pump Operating Manual ).
Pump head
compression values not
reached Check the compression values and take remedial action (refer to Pump Operating
Manual ).
System clogged with
particles Samples and insufficient solvent purity are frequently the source for particles
clogging the system. Follow the instructions for resolving clogging in the
autosampler (refer to
Split Sampler Operating Manual ).
High backpressure
Possible Cause Remedial Action
One or more capillaries
in the system clogged
or damaged by bending Check the capillaries in the system systematically from the detector to the pump.
Replace the capillaries as needed.
Pump inline filter (or
static mixer) clogged Check the permeability of the inline filter (or static mixer). Replace the inline filter
(or static mixer) if necessary (refer to
Pump Operating Manual ).
Pre-heater clogged Rinse the pre-heater capillary in reverse direction, using an appropriate solvent. If
necessary, replace the pre-heater (refer to
Column Compartment Operating
Manual ).
Column switching valve
clogged Check the valve for indications of blockage. Replace the valve if necessary (refer to
Column Compartment Operating Manual ).
Post-column cooler
clogged or defective Rinse the post-column cooler capillary in reverse direction using an appropriate
solvent. If necessary, replace the post-column cooler (refer to
Column
Compartment Operating Manual ).
Flow cell clogged Clean the flow cell. Replace the flow cell if necessary (refer to Detector Operating
Manual ).
Contamination in the
system Flush the system with an appropriate solvent.
Low backpressure
Possible Cause Remedial Action
Leak in system Find and eliminate the leak.