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5

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Installation

Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual

Page 51

5.4

Accessing the Interior Components

To access the interior components in the device, open the front doors.
To allow easy access from the front, the user-accessible components and
flow connections in the device are located directly behind the doors.

Figure 5: Opening the front doors

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Summary of Contents for thermoscientific Vanquish Pumps C

Page 1: ...thermoscientific Vanquish Pumps C Pumps F VC Pxx VF Pxx Operating Manual 4820 4405 EN Revision 4 0 December 2019 ...

Page 2: ...pt with the written authorization of Thermo Fisher Scientific Inc This manual is provided as is The contents of this manual are subject to being changed without notice in future revisions Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free Thermo Fisher Scientific Inc assumes no responsibility and will not be liable for any errors omissions d...

Page 3: ...r Scientific sales organization For contact information go to Contact Us on http www thermofisher com Technical Assistance For technical support for HPLC products contact your local Thermo Fisher Scientific support organization For contact information go to Contact Us on http www thermofisher com Contacting Us Pumps C Pumps F VC Pxx VF Pxx Operating Manual Page 3 ...

Page 4: ...Contacting Us Page 4 Pumps C Pumps F VC Pxx VF Pxx Operating Manual ...

Page 5: ... the Device 19 2 1 4 Rating Plate 19 2 2 Intended Use 20 2 3 Safety Precautions 21 2 3 1 General Safety Information 21 2 3 2 Qualification of the Personnel 22 2 3 3 Personal Protective Equipment 22 2 3 4 Electrical Safety Precautions 23 2 3 5 General Residual Hazards 24 2 3 6 In Case of Emergency 25 2 4 Solvent and Additive Information 26 2 4 1 General Compatibility 26 2 4 2 Allowed pH Ranges 27 2...

Page 6: ... Arrangement 52 5 5 2 Connecting the Device 53 5 5 3 Connecting the Power Cord 55 5 6 Setting Up the Flow Connections 56 5 6 1 General Information and Guidelines 56 5 6 2 Guiding Capillaries and Tubing Through the System 57 5 6 3 Connecting Fittings Capillaries and Tubing 59 5 6 4 Guiding Liquids to Waste 64 5 6 5 Connecting the Solvent Lines 64 5 6 6 Installing Solvent Shut Off Valves 69 5 7 Seal...

Page 7: ...lvent Shut Off Valves 110 6 10 Shutting Down the Device 113 6 10 1 Short Term Shutdown Interruption of Operation 113 6 10 2 Long Term Shutdown 114 6 10 3 Restart after Long Term Shutdown 116 7 Maintenance and Service 117 7 1 Introduction to Maintenance and Service 118 7 2 Safety Guidelines for Maintenance and Service 119 7 3 General Rules for Maintenance and Service 121 7 4 Routine and Preventive ...

Page 8: ...Static Mixer for Permeability 168 7 8 2 Replacing the Static Mixer or Capillary Mixer 169 7 9 Solvent Lines and Solvent Line Filters 171 7 9 1 Emptying the Solvent Lines 171 7 9 2 Replacing Solvent Lines 172 7 9 3 Replacing the Solvent Line Filter 175 7 10 Replacing the Purge Valve Knob 177 7 11 Testing the Pump for Leakage General Test 179 7 12 Replacing the Main Power Fuses 182 7 13 Updating the...

Page 9: ... Operating Principle 221 9 2 3 Dwell Volume of the Pump 222 9 2 4 Setting the Solvent Composition 223 9 2 5 Synchronizing the Injection Time with the Pump Strokes 224 9 2 6 Dual Pump Specifics 225 9 3 Binary Pump 228 9 3 1 Interior Components 228 9 3 2 Operating Principle 230 9 3 3 Dwell Volume of the Pump 231 9 3 4 Setting the Solvent Composition 232 9 4 Isocratic Pump 233 9 4 1 Interior Componen...

Page 10: ... 266 12 2 4 Quaternary Pump 267 12 3 Optional Accessories 269 12 3 1 Optional Accessories Miscellaneous 269 12 3 2 Optional Mixing Systems and Inline Filters 270 12 4 Consumables and Replacement Parts 273 12 4 1 Maintenance Kits 273 12 4 2 Pump Heads and Components 277 12 4 3 Solvents and Wash Systems 280 12 4 4 Mixing Systems 282 12 4 5 Miscellaneous Parts 282 12 4 6 Interface Cables and Power Co...

Page 11: ...s F VC Pxx VF Pxx Operating Manual Page 11 1 Using this Manual This chapter provides information about this manual the conventions used throughout the manual and the reference documentation that is available in addition to this manual ...

Page 12: ...erly Note the following The device configuration may vary therefore not all descriptions necessarily apply to your particular device If some detail applies to only one model or variant the model or variant is identified by name Illustrations in this manual are provided for basic understanding They can vary from the actual model of the device or component However this does not influence the descrip...

Page 13: ...section or procedure appear in the section or procedure to which they apply They appear different from the main flow of text Safety messages are often preceded by an alert symbol and or alert word The alert word appears in uppercase letters and in bold type Make sure that you understand and follow all safety messages presented in this manual 1 2 2 Special Notices and Informational Notes Special no...

Page 14: ...ermo Chromeleon 7 Services Manager Start Instrument Controller References and Messages References to additional documentation appear italicized Messages that appear on the screen are identified by quotation marks Viewpoint If not otherwise stated the expressions left and right in this manual always refer to the viewpoint of a person that is facing the device from the front Particularly Important W...

Page 15: ...e Acrobat is required Software Documentation Additional software documentation includes the following Chromeleon Help and documents The Chromeleon Help provides extensive information and comprehensive reference material for all aspects of the software In addition the following documentation is available availability depends on the software version Installation Guide For basic information about dev...

Page 16: ...perating Manual TIP The Chromeleon Help and documents are included in the software shipment Third Party Documentation Refer also to the user documentation provided by the manufacturers of third party components and materials for example Safety Data Sheets SDSs ...

Page 17: ...2 Safety Pumps C Pumps F VC Pxx VF Pxx Operating Manual Page 17 2 Safety This chapter provides general and specific safety information and informs about the intended use of the device ...

Page 18: ...N Indicates a hazardous situation that if not avoided could result in minor or moderate injury WARNING Indicates a hazardous situation that if not avoided could result in serious injury 2 1 2 Observing this Manual Observe the following Before installing or operating the device read this manual carefully to be familiar with the device and this manual The manual contains important information with r...

Page 19: ...r to prevent damage to the device Ο Power supply is on Power supply is off Indicates alternating current 2 1 4 Rating Plate The rating plate is present on the device near the electrical connections The rating plate indicates the serial number part number module name revision number if any line and fuse rating and the manufacturer s address TIP An additional type label on the leak tray of the devic...

Page 20: ...em are intended to be used as General Laboratory Equipment GLE They are not intended for use in diagnostic procedures Laboratory Practice Thermo Fisher Scientific recommends that the laboratory in which the Vanquish system is used follow best practices for LC analyses This includes among others Using appropriate standards Regularly running calibration Establishing shelf life limits and following t...

Page 21: ...nents options and peripherals specifically authorized and qualified for the device by Thermo Fisher Scientific Perform only the procedures that are described in this operating manual and in supporting documents for the device Follow all instructions step by step and use the tools recommended for the procedure Open the enclosure of the device and other components only if specifically instructed to ...

Page 22: ...ystem General Operation The device is designed to be operated only by trained and qualified personnel in a laboratory environment All users must know the hazards presented by the device and the substances they are using All users should observe the related Safety Data Sheets SDSs 2 3 3 Personal Protective Equipment Wear personal protective equipment and follow good laboratory practice to protect y...

Page 23: ...ice High voltages are present inside the device that could cause an electric shock or damage to the device Do not make any changes to the electrical or grounding connections If you suspect any kind of electrical damage disconnect the power cord and contact Thermo Fisher Scientific Technical Support for assistance Do not open the housing or remove protective panels unless specifically instructed to...

Page 24: ...e volume of substances to the minimum volume required for sample analysis Do not operate the device in a potentially flammable environment Avoid accumulation of harmful substances Make sure that the installation site is well ventilated Dispose of hazardous waste in an environmentally safe manner that is consistent with local regulations Follow a regulated approved waste disposal program WARNING Bi...

Page 25: ...erate the device in the presence of flammable gases or fumes CAUTION Allergic Reaction Some capillaries in the Vanquish system are made of MP35N a nickel cobalt based alloy Individuals with sensitivity to nickel cobalt may show an allergic reaction from skin contact CAUTION Sparking due to Electrostatic Discharge Liquid flowing through capillaries can generate static electricity This effect is par...

Page 26: ...solvents and additives that are compatible with all parts in the flow path TIP In a Vanquish Core system normal phase NP compatible solvents and additives may be used if the VC pumps and the VC autosamplers are modified with the components from the Normal Phase NP kit See Normal Phase Compatible Solvents and Additives page 249 NOTICE If the system flow path includes a Vanquish charged aerosol dete...

Page 27: ... system disconnect the detector from the system Mobile phases containing ammonium hydroxide In rare cases a shortened lifetime of reversed phase UHMW PE piston seals has been observed with high pH ammonium hydroxide containing mobile phases and prolonged exposure Vanquish Horizon Vanquish Flex 2 12 2 4 3 Allowed Concentrations Allowed concentrations standard system configuration System Standard Co...

Page 28: ...th of the other modules in the Vanquish system refer to the Specifications chapter in the Operating Manual for the modules Follow any specific recommendations presented in other sections of this manual Refer also to the operating manuals for all modules in the Vanquish system They may provide additional guidelines and information Observe the general guidelines and recommendations on the use of sol...

Page 29: ...at you make to the device may void compliance with one or more of these EMC and safety standards Changes to the device include replacing a part or adding components options or peripherals not specifically authorized and qualified for the product by Thermo Fisher Scientific To ensure continued compliance with EMC and safety standards replacement parts and additional components options and periphera...

Page 30: ...2 Safety Page 30 Pumps C Pumps F VC Pxx VF Pxx Operating Manual ...

Page 31: ...3 Device Overview Pumps C Pumps F VC Pxx VF Pxx Operating Manual Page 31 3 Device Overview This chapter introduces you to the device and the main components ...

Page 32: ...es the piston seals automatically This prevents crystallization and particle deposits on the piston surfaces and seals which may damage the components Built in vacuum degasser for enhanced flow stability and best detector sensitivity all pumps except isocratic pump Mixing system with capillary mixer and static mixer for smoother baselines With the isocratic pump the mixing system serves as inline ...

Page 33: ...ith the increasing working pressure because part of the delivery stroke is required for compressing the liquid in the working cylinder Pulsation during the pre compression phase is reduced to a minimum by velocity modulation of the drive Highly constant delivery is ensured by a patented secondary control system automatic compressibility compensation The flow rate at atmospheric pressure is always ...

Page 34: ...F Pxx Operating Manual 3 3 Interior Components The user accessible components of the pump are located directly behind the front doors For pump specific pictures that provide an overview of the interior components see Pump Specific Information page 215 ...

Page 35: ...by preventing crystallization and particle deposits on the piston surfaces and seals which may otherwise damage the pistons and seals The picture shows the flow path through the seal wash system Figure 1 Flow path through the seal wash system left isocratic pump quaternary pump right binary pump dual pump No Description 1 Seal wash line from autosampler 2 Seal wash pump peristaltic pump 3 Peristal...

Page 36: ...example here in binary pump No Description 1a Isocratic pump dual pump quaternary pump Capillary from the pump head Binary pump Capillary from the right pump head 1b Binary pump only Capillary from the left pump head 2 System pressure sensor 3 Capillary mixer 4 Purge outlet 5 Purge valve knob Binary pump With the binary pump the purge sensor unit also combines the solvent streams from the pump hea...

Page 37: ... in smoother baselines The pump is shipped with the following mixing system Pump Mixing System Binary VC pump Quaternary pump Dual pump Mixing system volume 400 µL comprising Static mixer volume 350 µL Capillary mixer volume 50 µL The dual pump has two mixing systems Binary VF pump Isocratic pump Mixing system volume 200 µL comprising Static mixer volume 150 µL Capillary mixer volume 50 µL With th...

Page 38: ... liquid is collected in the leak tray and guided to the drain port From the drain port the liquid is discharged to waste through the drain system of the Vanquish system When the leak sensor detects leakage the status indicators change to red and beeping starts to alert you Follow the instructions in this manual to find and eliminate the source for the leakage If leakage is not resolved after 3 min...

Page 39: ...ted sample analysis with the Chromeleon software refer to the Vanquish System Operating Manual Details on control and operation of the device are available in the Chromeleon Help TIP The device can be operated also with other data systems such as Thermo ScientificTM XcaliburTM In this case installation of additional software is required in addition to the data system software For details contact t...

Page 40: ...3 Device Overview Page 40 Pumps C Pumps F VC Pxx VF Pxx Operating Manual ...

Page 41: ...4 Unpacking Pumps C Pumps F VC Pxx VF Pxx Operating Manual Page 41 4 Unpacking This chapter provides information for unpacking the device and informs you about the scope of delivery ...

Page 42: ...r one person alone to handle safely To avoid personal injury or damage to the device observe the following guidelines Physical handling of the device including lifting or moving requires a team effort of two persons A team effort is in particular required when lifting the device into the system stack or when removing it Use the carrying handles that were shipped with the device to move or transpor...

Page 43: ... it in the system stack see System Arrangement page 52 7 On each carrying handle loosen the attachment screw until the carrying handle is moveable in the rail Do not remove the screws from the carrying handles completely 8 Slide off the carrying handles from the rails towards the rear of the device Figure 4 Sliding off the carrying handle from the left rail TIP Keep the shipping container the carr...

Page 44: ...Operating Manual 4 2 Scope of Delivery The following items are included in the delivery Pump Ship Kit Operating manual Power cord For information on contents of the ship kit or reordering parts see Accessories Consumables and Replacement Parts page 261 ...

Page 45: ...Pxx VF Pxx Operating Manual Page 45 5 Installation This chapter specifies the requirements for the installation site and describes how to set up install and configure the device in the Vanquish system and in the chromatography software ...

Page 46: ...ort is in particular required when lifting the device into the system stack or when removing it Use the carrying handles that were shipped with the device to move or transport the device Never move or lift the device by the front doors This will damage the doors or the device CAUTION Electric Shock or Damage to the Device After the power to the device is turned off the device is still energized as...

Page 47: ... 46 and Site Requirements page 49 2 Set up the device hardware See Setting Up the Hardware page 52 3 Set up the flow connections See Setting Up the Flow Connections page 56 4 Turn on the device See Turning On the Device page 81 TIP Before turning on the power to a Vanquish system module for the first time verify that the chromatography software is installed on the data system computer When the pow...

Page 48: ...s provide information about the parameters to be adapted and the required settings 7 Recommended Perform Operational Qualification The qualification kit includes all materials required for the qualification and detailed instructions Moving the Device after Installation If you have to move the device after it has been set up and installed in the Vanquish system prepare the device for transport and ...

Page 49: ...s stated in the Vanquish System Operating Manual For specifications see Specifications page 253 and the Specifications sections in the Operating Manuals for the other modules in the Vanquish system For general residual hazards see General Residual Hazards page 24 5 3 1 Power Considerations The power supply of the device has wide ranging capability accepting any line voltage in the range specified ...

Page 50: ...et that has a protective ground connection In case of emergency it must be possible to reach the power cord easily at any time to disconnect the device from the power line WARNING Electric Shock or Damage to a Product Misuse of the power cords could cause personal injury or damage the instrument Use the power cords provided by Thermo Fisher Scientific only for the purpose for which they are intend...

Page 51: ...Accessing the Interior Components To access the interior components in the device open the front doors To allow easy access from the front the user accessible components and flow connections in the device are located directly behind the doors Figure 5 Opening the front doors ...

Page 52: ...m Arrangement The device is part of the Vanquish system The system modules are typically arranged in a system stack with the arrangement depending on the system configuration Figure 6 Vanquish system standard configuration example No Description 1 Solvent Rack 2 Detector 3 Autosampler 4 Pump 5 System Base 6 Column Compartment For instructions on how to set up the system stack refer to the Vanquish...

Page 53: ...and the manufacturer s address 2 Main power switch on off control 3 Fuse holder 4 Power inlet connector 5 System Interlink port Allows power on off control for the device from the Vanquish system base and device communication 6 Digital I O ports Dig I O Allow exchange of digital signals with external instruments Each digital I O port provides one input one relay output and one bidirectional input ...

Page 54: ...nality Follow these steps NOTICE Never use defective communication cables If you suspect that a cable is defective replace the cable To ensure trouble free operation use only the cables provided by Thermo Fisher Scientific for connecting the device 1 Place the device in the system as required by the system configuration For details refer to the Vanquish System Operating Manual 2 Connect the requir...

Page 55: ...sure that no condensation is present in the devices If you suspect that condensation is present allow the device to warm up to room temperature slowly Wait until the condensation is completely gone before proceeding 1 Verify that the power switch on the device is set to OFF 2 Connect the power cord to the power inlet connector on the device 3 Connect the free end of the power cord to an appropriat...

Page 56: ...e of the modules and entire system or can even cause damage to the modules and system Therefore Always wear appropriate gloves Place the components only on a clean lint free surface Keep your tools clean Use only lint free cloth for cleaning For installation instructions and guidelines and for handling recommendations see Connecting Fittings Capillaries and Tubing page 59 NOTICE When you install d...

Page 57: ...nd Tubing Through the System Flow connections between the modules of the Vanquish system are guided through either the tubing chase in the devices or the guide holes or capillary clips of the devices Tubing Chase Tubing Guide Tubing Bracket To guide certain tubes and lines solvent tubing wash liquid tubing detector waste line from the top module to the bottom module in the Vanquish system stack th...

Page 58: ...et left tubing bracket installed right Dual System Arrangements The number of tubes may exceed the capacity that the tubing guides can hold In this case it is recommended to place the solvent lines in the tubing guides and route any additional tubes freely in the tubing chase Guide Holes and Capillary Clips Guide holes and capillary clips are provided at specific positions on the system modules Ro...

Page 59: ...tors must be free from contaminants Even minute particles may cause damage to the system or lead to invalid test results Do not install capillaries or tubes that are stressed nicked kinked or otherwise damaged Install capillaries and fittings only at the positions for which they are intended Follow the connecting instructions as required by the fitting connection For Viper connections see Connecti...

Page 60: ...onnect Viper fittings with torque toothing follow these steps NOTICE Tighten or loosen Viper capillaries only with your fingers Do not use tools other than the knurl that comes with the capillary To avoid damage to the capillary or connection tighten and loosen the Viper capillaries only when the system pressure is down to zero Mind the correct orientation of the tool when tightening or loosening ...

Page 61: ...ings with Torque Toothing 1 Remove the capillary 2 Clean the capillary ends carefully by using a lint free tissue wetted with isopropanol 3 Reinstall the capillary 4 If the connection continues to leak install a new Viper capillary To loosen a connection use this side of the installation tool Figure 13 Tool on torque toothing for loosening connections Connecting Viper Fittings with Knurls To conne...

Page 62: ...n easily remove the knurls from neighboring capillaries through this slot and attach them again later 3 Check whether the connection leaks If leakage exists follow the steps further down Resolving Leakage of Viper Fittings with Knurls 1 Tighten the connection a little more 2 If leakage continues remove the capillary 3 Clean the capillary ends carefully by using a lint free tissue wetted with isopr...

Page 63: ... may damage the connection With a torque wrench the torque should not exceed 3 Nm for steel capillaries 3 Check whether the connection leaks If leakage exists follow the steps further down Resolving Leakage with Conventional Fitting Connections 1 Remove the capillary 2 Clean the connection port with a cleaning swab 3 Reinstall the capillary 4 If the connection continues to leak install a new capil...

Page 64: ...1 Waste line from purge unit routing path to drain port outlet example 2 Drain port 3 Drain port outlet to Vanquish drain system 4 Leak sensor For information about how the liquid is discharged to waste through the Vanquish drain system refer to the Vanquish System Operating Manual 5 6 5 Connecting the Solvent Lines The pump is shipped from the manufacturing site with the solvent lines installed b...

Page 65: ...tion page 215 Parts and tools required Solvent reservoir assemblies including reservoir reservoir cap cap plugs and retaining guide for the solvent line Solvent lines Solvent line filters Optional to shut off the solvent flow through the system at the solvent reservoir Shut off valve kit containing one shut off valve and fitting connections Tubing cutter Preparations Figure 16 Solvent line filter ...

Page 66: ... lower degas chambers remove the seal wash detector assembly funnel and detector Grasp and pull the assembly upward by the funnel The detector including the tubing and cable can remain connected to the funnel Figure 17 Removing the seal wash detector assembly No Description 1 Funnel seal wash detector 2 Seal wash detector 3 Detector cable 4 Seal wash line Follow these steps Connecting the solvent ...

Page 67: ...olvent lines and in the pump 2 Route the solvent lines from the pump inlet to the solvent rack through the tubing guides in the pump and all modules above the pump in the system stack Observe the routing guidelines in Guiding Capillaries and Tubing Through the System page 57 3 Route the solvent lines through the guide hole in the solvent rack and fix them in the appropriate tubing guides in the so...

Page 68: ... the reservoir caps with the cap plugs 4 Slide a filter holder onto each solvent line 5 Fill the solvent reservoirs with solvent observing the guidelines and recommendations in Use of Solvents and Additives page 92 Before using a solvent reservoir for the first time rinse the reservoirs thoroughly Use a high purity solvent for rinsing 6 Tighten the reservoir cap hand tight Press the retaining guid...

Page 69: ...l operation of the pump follow the steps in Installing Solvent Shut Off Valves page 110 When To shut off the solvent flow through the system at the solvent reservoir for example to prevent solvent from flowing through the system when you open a flow connection on the low pressure side Parts and additional items required Shut off valve kit containing one shut off valve and fitting connections Tubin...

Page 70: ...ee end of each solvent line slide a fitting and a ferrule Mind the correct orientation of the fitting and ferrule see picture 3 Tighten the solvent lines to the shut off valve Figure 21 Solvent line with shut off valve installed No Description 1 Shut off valve 2 Retaining guide 4 Check the solvent lines across the entire flow path Make sure that the lines are not bent pinched or squeezed at any po...

Page 71: ...seal wash system see Setting Up the Seal Wash System page 72 NOTICE If you want to use normal phase compatible solvents and additives in a Vanquish Core system you must modify the VC pump and VC autosampler with the components from the Normal Phase NP kit See Normal Phase Compatible Solvents and Additives page 249 5 7 1 Choosing the Seal Wash Liquid Use seal wash liquid that fulfills the following...

Page 72: ...ing device head Seal wash line silicone tubing for connection to the seal wash reservoir NOTICE If you want to use normal phase compatible solvents and additives in a Vanquish Core system you must modify the VC pump and VC autosampler with the components from the Normal Phase NP kit See Normal Phase Compatible Solvents and Additives page 249 TIP To avoid the formation of gas bubbles in the solvent...

Page 73: ...ption 1 Seal wash reservoir 2 Seal wash lines in the autosampler thick tubing with 2a Wash line from reservoir to inlet port 2b Seal wash inlet port right tubing connector 2c Wash line from inlet port to metering device head 2d Wash line to seal wash pump 3 Metering device head 4 Seal wash pump peristaltic pump 5 Seal wash lines in the pump thin tubing with 5a Wash lines from seal wash pump to pum...

Page 74: ... system comprises the following steps 1 Engaging the peristaltic tubing 2 Connecting the seal wash line between autosampler and pump 3 Routing the seal wash line to the solvent rack 4 Connecting the seal wash line to the seal wash reservoir 5 Flushing the seal wash system Follow the steps in the respective sections further down Engaging the Peristaltic Tubing In the pump engage the peristaltic tub...

Page 75: ...al Wash Line between Autosampler and Pump 1 In the autosampler locate the seal wash line clear silicone tubing to the pump Single split sampler The seal wash inlet port is located on the top enclosure frame right tubing connector The seal wash line to the pump is interconnected to the seal wash inlet port Dual split sampler The seal wash inlet port is located on the right enclosure frame top tubin...

Page 76: ...n the free end of the peristaltic tubing Routing the Seal Wash Line to the Solvent Rack 1 Locate the seal wash line for connection from the autosampler to the seal wash reservoir 2 Connect the seal wash line on the autosampler Single split sampler Connect the line to the seal wash inlet port on the top enclosure frame right tubing connector Dual split sampler Connect the line to the seal wash inle...

Page 77: ...for seal wash line 3 4 Not to be used for wash liquid line reserved for other tubing Connecting the Seal Wash Line to the Seal Wash Reservoir Figure 26 Seal wash line and reservoir cap No Description 1 Seal wash line 2 Retaining guide 3 Reservoir cap 4 Cap plugs 1 Feed the seal wash line through the retaining guide 2 Feed the seal wash line through an open hole in the cap of the seal wash reservoi...

Page 78: ...the flow path Flushing the Seal Wash System To flush the seal wash system follow the procedure in Flushing the Seal Wash System page 78 5 7 3 Flushing the Seal Wash System To flush the seal wash system select one of the following alternatives Turn on the pump Whenever the pump is turned on the pump starts operation with a seal wash cycle Flush the seal wash system from the Chromeleon software Flus...

Page 79: ...open end of the tubing 3 To allow the liquid to travel easily through the seal wash system press down the lever of the seal wash pump and hold it in that position with one hand 4 Pull out the syringe plunger to draw seal wash liquid into the tubing 5 Release the lever of the seal wash pump when the liquid reaches the syringe 6 Remove the syringe and reconnect the tubing to the connection port on t...

Page 80: ... holes in the autosampler and pump left view from VF VH autosampler example right view from pump No Description 1 Capillary connecting the pump and autosampler 1a Capillary end connected to the autosampler injection valve 1b Capillary end to be connected to pump outlet 2 Guide holes in autosampler housing and pump housing 2a Guide hole in autosampler housing view from above 2b Guide hole in pump h...

Page 81: ...e system base 2 Turn on the device with its main power switch Turn the device off with the main power switch when instructed to do so for example during maintenance Pressing the system power button will not be sufficient to turn off the power to the device completely When Powering Up the Pump Whenever the power to the pump is turned on the following sequence of events occurs The pump performs a se...

Page 82: ... chromatography software is already installed on the data system computer and a valid license is available For more information about setting up the Vanquish system in the software refer to the Vanquish System Operating Manual The Help for the software that you are using provides detailed information about the settings on each property page ...

Page 83: ...6 Operation Pumps C Pumps F VC Pxx VF Pxx Operating Manual Page 83 6 Operation This chapter describes the elements for device control provides information for routine operation and for shutdown ...

Page 84: ...t the case refer to the instructions in Installation page 45 For a basic description of instrument control and automated sample analysis with the Chromeleon software refer to the Vanquish System Operating Manual Details on control and operation of the device are available in the Chromeleon Help Software descriptions in this manual refer to Chromeleon 7 Terminology may be different to that of other...

Page 85: ...ttention also to the following guidelines When operating the chromatography system always set the lower pressure limit for the pump This prevents damage resulting from leakage or from running the pump dry If there is evidence of leakage in the device turn off the pump flow and remedy the situation immediately If the pump flow is interrupted act appropriately to protect the components in the detect...

Page 86: ...he device Pump Block Status LED The status indicator LED is located inside the pump above the pump head pump block LED The LED indicates the operational status of the pump block 6 3 1 Keypad The keypad inside the device allows you to perform certain functions directly from the device When you press a button a short beep confirms that the function is performed When the device is connected in the Ch...

Page 87: ...URGE function from the keypad select the pump head for which you want to perform the action Isocratic pump and quaternary pump The button is disabled DOCK Pressing the button moves the pistons of the selected pump head to the appropriate position for maintenance procedures such as pump head or piston replacement and undocks the pistons When undocking is complete the LED next to the FLOW button sta...

Page 88: ...ftware The button functionality is as follows when the device is connected in the Chromeleon software No injection or sequence is running All functions are available from the keypad An injection or sequence is running The MUTE ALARM function remains available from the keypad allowing you to turn off the beep for the current alarm 6 3 2 Status Indicators This section provides information about the ...

Page 89: ...ow is on Blue An injection or sequence is running including data acquisition Red A problem or error has occurred For the related message check the Chromeleon Audit Trail For remedial action see Troubleshooting page 197 STATUS LED The STATUS LED on the keypad inside the device provides the following information STATUS LED Description Off dark The power to the device is turned off Green The device i...

Page 90: ...about the compression values see Checking the Compression Values page 210 Green flashing during pump maintenance The pistons are in the appropriate position for piston seal replacement Red flashing The pistons are not in the appropriate position for normal operation for example after piston seal replacement or an error occurred during internal maintenance see Internal Maintenance page 122 In both ...

Page 91: ... system power button stands out If the main power switch on a device is off you cannot turn on the device with the system power button To turn off a device completely you have to turn it off with the main power switch on the device Pressing the system power button will not be sufficient to turn off the power to the device completely When Powering Up the Pump Whenever the power to the pump is turne...

Page 92: ...mplete use membrane filtration 0 2 µm to remove any particulate matter and to reduce microbial growth When using water use high quality water for example UHPLC grade or LC MS grade 0 2 µm filtered When you use water from water purification systems keep in mind that polymeric contamination may occur if the purification system is not maintained properly Before filling a solvent reservoir always rins...

Page 93: ... intervals VF pumps The pumps are shipped with UHMW PE piston seals Using tetrahydrofuran ketones or ammonium hydroxide as solvents can damage the seals Under harsh conditions consider replacing the UHMW PE piston seals with carbon fiber filled PTFE piston seals part no 6040 0306 In this case also install new pistons Exchanging the piston seals but keeping the pistons that were used with the UHMW ...

Page 94: ... Manual TIP In a Vanquish Core system normal phase NP compatible solvents and additives may be used if the VC pumps and the VC autosamplers are modified with the components from the Normal Phase NP kit See Normal Phase Compatible Solvents and Additives page 249 ...

Page 95: ...he manufacturing site When operating the device for the first time use solvents that are miscible with isopropanol If they are not use an appropriate intermediate solvent To remove the isopropanol from the device components Verify that the flow cell and the column are not connected in the flow path Purge the pump and then have the pump deliver flow for a short time Verify that any air bubbles are ...

Page 96: ...statting in the autosampler Flow cell in a fluorescence detector Evaporation tube in a charged aerosol detector Turning on the lamp or lamps in the UV VIS detector Monitoring the pump pressure and pressure ripple and checking that the pressure is stable and the ripple within reasonable limits for the application Monitoring the detector signal and checking whether the detector signal is stable so t...

Page 97: ...ts delivering with the selected flow rate If the value is too low it will take correspondingly long for the pump to build up the necessary pressure and start delivery the required flow If the value is too high this may impair the column lifetime Flow deceleration The flow deceleration Maximum Flow Ramp Down determines how fast the pump reduces the flow If the value is too low it will take correspo...

Page 98: ...omeleon software The Chromeleon software uses this setting to generate the channel for recording the pump pressure which corresponds to the column pressure With the dual pump the check boxes are labeled PumpLeft_Pressure for the left pump and PumpRight_Pressure for the right pump Always record the pump pressure If a problem occurs the information from the pump pressure channel can provide helpful ...

Page 99: ... see Optional Accessories page 269 For monitoring from the Chromeleon software enter the following information Volume of solvent in the reservoir at the beginning of a sequence Lower limit for the solvent in the reservoir which is the minimum volume of liquid that must be available in the reservoir The Remain Time property for the solvent reports the time left until the liquid level is expected to...

Page 100: ...id in the waste container at the beginning of a sequence Upper limit for the liquid level in the waste container The Remain Time property for the waste reports the time left until the liquid level is expected to reach the upper limit The Chromeleon software calculates the time from the current flow rate and calculated current liquid level Warning limit when you want to be informed about the liquid...

Page 101: ... convex Curves 6 through 9 are concave upward Concave curves cause slower changes in solvent composition at the beginning of the gradient and rapid changes at the end Slope changes over time become extreme as curves go from 6 least concave to 9 most concave In addition you can define step gradients directly in the gradient table for example step A and step B Thus more than 11 different options are...

Page 102: ...de select Idle to stop a running wash cycle You can disable the drop counter functionality by setting Rear Seal Wash Monitoring to Disabled Note that disabling drop detection will not disable the seal wash system However messages relating to the seal wash system for example when the system has run out of wash liquid or piston seal leakage will no longer alert you in the user interface Synchronizat...

Page 103: ...annel to be purged and solvent to be used The purge cycle is performed with the most recently selected channel and eluent composition You can adapt the settings in the Chromeleon software if required The setting are considered also when purging the pump from the keypad To Purge the Pump The following alternatives are available Procedure Description Automatic purging Automatic purging runs without ...

Page 104: ...e the pump manually see Manual Purging page 105 When The pump is operated for the first time To flush out the isopropanol that is present in some components of the pump when the pump is shipped from the manufacturing site One or more solvent lines have been empty To remove air bubbles that might be trapped in the system pump head solvent lines because air bubbles in the system may lead to the foll...

Page 105: ...urge valve by hand to direct the solvent to waste through the purge outlet on the purge sensor unit The procedure depends on the reason for purging When the pump is operated for the first time or when solvent lines are empty follow the steps in Purging with First Time Operation or Empty Solvent Lines page 105 To remove air bubbles or to change to a different type of solvent or when using a fresh r...

Page 106: ...syringe tip into the open end of the tubing 4 Open the purge valve by turning the valve knob counterclockwise Follow these steps 1 In the Chromeleon software set the channel that you want to purge to 100 2 Optional In the Chromeleon software adapt the settings for the purge flow or purge time if required 3 Binary pump and dual pump For purging from the keypad verify that you have selected the pump...

Page 107: ... damage the purge valve seals 11 Empty the silicone tubing 12 Dual pump only If applicable perform the purge procedure also for the other pump Emptying the Silicone Tubing NOTICE Always empty the silicone tubing when purging is complete When the purge valve is closed purge liquid remains in the tubing connected to the purge outlet Leaving the liquid in the tubing for a longer period limits the tub...

Page 108: ...05 Items required Solvents suitable for purging Silicone tubing Tissue Preparations 1 To be able to generate negative pressure connect an autosampler or another type of flow restriction to the pump outlet If necessary close the pump outlet with a fitting plug 2 On the purge sensor unit attach silicone tubing to the purge outlet if it is not yet connected 3 Route the free end of the tubing to the d...

Page 109: ...silicone tubing when purging is complete When the purge valve is closed purge liquid remains in the tubing connected to the purge outlet Leaving the liquid in the tubing for a longer period limits the tubing lifetime 1 To absorb any liquid that might be leaving the purge outlet when the tubing is removed place the tissue under the outlet 2 On the purge sensor unit remove the silicone tubing from t...

Page 110: ...he built in vacuum degasser Purge the pump when changing to a different type of solvent or when restarting the pump after periods of inactivity TIP Shut off valves for solvent lines are available as an option see Installing Solvent Shut Off Valves page 110 These valves allow you to shut off the solvent flow through the system at the solvent reservoir for example to prevent solvent from flowing thr...

Page 111: ...for each solvent line in which you want to install a shut off valve Figure 30 Installing a shut off valve in the solvent line No Description 1 Solvent line 2 Fitting 3 Ferrule 4 Shut off valve 1 Unscrew the cap of the solvent reservoir and remove the cap together with the solvent line from the reservoir 2 Start a purge cycle as appropriate 3 Stop purging as soon as the solvent line is empty 4 Reti...

Page 112: ...a fitting and a ferrule Mind the correct orientation of the fitting and ferrule see picture 7 Tighten the solvent lines to the shut off valve 8 Check the solvent lines across the entire flow path Make sure that the lines are not bent pinched or squeezed at any point in the flow path 9 To remove any air bubbles from the solvent lines purge the pump Figure 31 Solvent line with shut off valve install...

Page 113: ...llowing Detector Type Description Charged aerosol detector Check that sufficient gas is available to continue gas flowing through the detector This is to prevent any build up of residue from solvents or analytes Gas must be flowing when pump flow is delivered to the detector UV VIS detectors The lamp s in the detector can remain turned on Variable wavelength detector and VH D10 diode array detecto...

Page 114: ...mal Phase Compatible Solvents and Additives page 251 Situation after Shutdown If no additive is used If an additive is used Device remains in the laboratory after shutdown Flush the system for example with methanol 100 acetonitrile should not be used Flush the system with several volumes of methanol and water 50 50 for example 1 0 mL min for 10 minutes with the standard system to prevent salt buil...

Page 115: ... system modules remain in the system stack and are to be turned off Turn off the system with the system power button on the system base Device shall be transported or shipped after shutdown If one of the modules shall be removed from the system stack turn off all system modules with their main power switch Pressing the system power button will not be sufficient to turn off the power to the devices...

Page 116: ... retaining guide remains in the hole in the reservoir cap If it does not press the retaining guide into the hole to ensure that the tubing is kept in place in the cap 4 Turn on the device Observe the following Situation Action If the device remained in the system stack and all system modules were turned off Turn on the system with the system power button on the system base If the device is restart...

Page 117: ...aintenance and Service Pumps C Pumps F VC Pxx VF Pxx Operating Manual Page 117 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that the user may perform ...

Page 118: ... brevity referred to as Thermo Fisher Scientific service personnel The device is designed for easy maintenance and service The user serviceable parts of the device can be accessed from the front If not stated otherwise the maintenance procedures do not require that you remove the device from the system The maintenance procedures do not require that you remove the doors However it is possible to re...

Page 119: ...can pose health risks Solvent can spray when capillaries burst slip out of their fittings or are not properly tightened or when capillary connections are otherwise open Wear appropriate protective equipment and follow good laboratory practice Before starting maintenance or repair procedures flush out harmful substances with an appropriate solvent WARNING Tilting Liquid Reservoirs Liquids in the re...

Page 120: ...nd remove the solvent lines together with the caps from the reservoirs Empty the solvent lines See Emptying the Solvent Lines page 171 Retighten the reservoir caps CAUTION Electric Shock or Damage to the Device After the power to the device is turned off the device is still energized as long as the power cord is connected Repair work on the device while the device is connected to power could lead ...

Page 121: ...g the flow path to replace capillaries in the system turn off the pump flow and wait until the system pressure is down to zero Dirty components can contaminate the chromatography system Contamination leads to poor performance of the modules and entire system or can even cause damage to the modules and system Therefore Always wear appropriate gloves Place the components only on a clean lint free su...

Page 122: ...e cycle 7 4 2 Maintenance Plan Perform the maintenance procedures in the table on a regular basis The frequency given in the table is a suggestion The optimum frequency for maintenance depends on several factors such as the types and amounts of samples and solvents used with the device Frequency What you should do Daily Inspect the flow connections for signs of leakage or blockage Inspect the flow...

Page 123: ...sh the degasser Check that all warning labels are still present on the device and clearly legible If they are not contact Thermo Fisher Scientific for replacement Annually Clean the check valves in an ultrasonic bath see Cleaning the Check Valves page 167 Replace the seal wash seals see Replacing the Seal Wash Seals page 154 Replace the seal wash lines see Replacing the Seal Wash Lines page 131 In...

Page 124: ...UHPLC grade or LC MS grade that are replaced frequently Regularly flushing the degassing channels with fresh solvent is usually sufficient If you use water acetonitrile or methanol for your chromatography flushing the channels once a week is an appropriate interval Mobile phases containing salts buffering agents and or other additives are inherently prone to organic contamination and or biodegrada...

Page 125: ... HPLC grade New solvent reservoirs with fresh solvent New frits for solvent line filters Follow these steps 1 Turn off the pump flow and wait until the system pressure is down to zero 2 Install the backpressure capillary on the pump outlet Guide the free end of the capillary to the waste container 3 Replace the frits in the solvent line filters with new frits 4 Replace the installed waste containe...

Page 126: ...iquids can cause damage Decontamination Decontamination is required for example when leakage or spillage has occurred or before service or transport of the device Use a suitable cleaning detergent or disinfectant to ensure that the treatment renders the device safe to handle Parts required Suitable cleaning detergent or disinfectant Purified water Lint free cloths or wipes CAUTION Explosive Gas Mi...

Page 127: ... a solution of lukewarm water and a suitable cleaning detergent 2 Allow the cleaning detergent to react as recommended by the manufacturer 3 Wipe the cleaned surfaces with purified water to ensure that all cleaning detergent residues have been removed 4 Wipe the surfaces dry using a soft lint free cloth or wipe 7 4 5 Predictive Performance General overview The Chromeleon software supports function...

Page 128: ... Predictive Performance counters for the pump Consider resetting these parameters after performing the related maintenance procedure Predictive Performance Command To perform CheckValvesServiceDone After replacement of the check valves For each pump head a common counter is available for all check valves inlet check valves and outlet check valves PistonsChanged After replacement of the pistons or ...

Page 129: ... wash system for leakage follow the steps in Testing the Seal Wash System for Leakage page 130 To replace the seal wash lines follow the steps in Replacing the Seal Wash Lines page 131 To replace the seal wash detector drop detector follow the steps in Replacing the Seal Wash Detector page 134 TIP To replace the seal wash seals which are part of the pump head see Replacing the Seal Wash Seals page...

Page 130: ...gure 32 Seal wash detector No Description 1 Seal wash detector 2 Tubing connection port port labeled in 2 Insert a syringe into the open end of the tubing 3 To allow the liquid to travel easily through the seal wash system press down the lever of the seal wash pump and hold it in that position with one hand 4 Pull out the syringe plunger to draw seal wash liquid into the tubing 5 When about 10 mL ...

Page 131: ...nal items required Seal wash lines as required Seal wash lines that connect the seal wash reservoir to the peristaltic tubing in the pump For more information refer to the Operating Manual for the Vanquish autosampler Seal wash lines that connect the components in the pump Peristaltic tubing PharMed tubing Fresh seal wash liquid For an overview of the seal wash system see the picture in Setting Up...

Page 132: ...the pump follow the steps in the Operating Manual for the Vanquish autosampler For the peristaltic tubing see Replacing the Peristaltic Tubing page 132 For the seal wash lines from peristaltic tubing to the pump heads and on to the seal wash detector see Replacing the Seal Wash Lines between the Peristaltic Tubing and the Seal Wash Detector page 133 Replacing the Peristaltic Tubing Figure 33 Seal ...

Page 133: ...re that the tubing is not pinched or squeezed in the holders 10 Retighten the reservoir cap hand tight Verify that the retaining guide remains in the hole in the reservoir cap If it does not press the retaining guide into the hole to ensure that the tubing is kept in place in the cap 11 Check the wash lines over the entire flow path a Make sure that no bending kink pinching or squeezing of the sea...

Page 134: ...he seal wash system using fresh seal wash liquid see Flushing the Seal Wash System page 78 7 5 3 Replacing the Seal Wash Detector When Contamination of detector electrodes Impaired functionality of the seal wash system Parts required Seal wash detector assembly consisting of the detector and funnel Follow these steps Figure 34 Removing the seal wash detector assembly No Description 1 Funnel seal w...

Page 135: ... If you have to assemble both parts for any reason push the detector into the funnel first Mind the orientation of the funnel Figure 35 Installing the seal wash detector assembly No Description 1a Funnel bottom side 1b Funnel top side 2 Seal wash detector 5 Reconnect the tubing to the seal wash detector port labeled in 6 Connect the detector cable to the DROP DET port 7 Flush the seal wash system ...

Page 136: ... Head page 138 To replace a piston see Replacing the Pistons page 144 To clean a piston see Cleaning the Piston page 146 To replace the piston seals or support ring see Replacing the Piston Seals or Support Ring page 147 To replace the seal wash seals see Replacing the Seal Wash Seals page 154 To replace the pump head seals see Replacing the Pump Head Seals page 157 To test the pump for piston sea...

Page 137: ...tion 1 Inlet check valve including 1a Valve nut 1b Valve cartridge 2 Pump head 3 Pump head seals 2 PTFE O ring seals 4 Piston seals 5 Support rings 6 Seal wash bodies pump head bushing 7 Seal wash seals 8 Seal wash plate 9 Pistons 10 Outlet check valve including 10a Valve nut 10b Valve cartridge 11 Connectors for seal wash tubing pluggable 12 U tube capillary from working cylinder to equilibration...

Page 138: ...t Viper capillary Installation tool for Viper capillaries with torque toothing Conventional capillary non Viper Open end wrench size 1 4 Solvent suitable for purging to flush out harmful substances Seal wash liquid Solvent suitable for your application Preparations 1 To flush out harmful substances purge the pump with an appropriate solvent 2 Turn off the pump flow and wait until the system pressu...

Page 139: ...e knob counterclockwise 2 As required by the pump type Pump Type Action Binary pump Select the pump head for which you want to perform the procedure Dual pump Select the pump for which you want to perform the procedure Isocratic pump quaternary pump Continue with the next step 3 Undock the pistons either from the keypad or from the Chromeleon software Wait until undocking is complete before procee...

Page 140: ...t may happen that the seal wash plate remains in the pump when you remove the pump head Follow these steps a Insert the spacing tool between the pump head and pump block b Carefully pull the seal wash plate away from the pump block Figure 38 Removing the seal wash plate 7 6 2 2 Installing the Pump Head Preparations 1 Remove the pump head See Removing the Pump Head page 139 2 Before you begin note ...

Page 141: ...n L for the left pump head or R for the right pump head To protect the pistons during shipment the spacing tool is installed between the seal wash plate and the pistons Remove the spacing tool Be careful not to pull out the pistons any further Figure 39 Removing the spacing tool No Description 1 Seal wash plate 2 Spacing tool 3 Pistons Follow these steps 1 Insert the pump head into the pump and ti...

Page 142: ...tions of leakage Tighten leaky connections when the system pressure is down to zero 9 In the Chromeleon software consider updating the Predictive Performance information for the pump head components 10 Optional Test the system wash system and pump for leakage see Testing the Seal Wash System for Leakage page 130 and Testing the Pump for Leakage General Test page 179 11 Recommended From the Chromel...

Page 143: ... Disconnect the tubing from the seal wash detector Figure 40 Seal wash detector No Description 1 Seal wash detector 2 Tubing connection port port labeled in 2 Insert a syringe into the open end of the tubing 3 To allow the liquid to travel easily through the seal wash system press down the lever of the seal wash pump and hold it in that position with one hand 4 Pull out the syringe plunger to draw...

Page 144: ...acing the Pump Head page 138 Follow these steps 1 Remove the pistons If the seal wash plate was removed together with the pump head Hold down the seal wash plate onto the pump head and pull out the pistons If the seal wash plate was removed separately Pull out the pistons from the seal wash plate 2 When reinstalling a piston that you removed Clean the piston Follow the steps in Cleaning the Piston...

Page 145: ...nections on the pump head for indications of leakage Tighten leaky connections when the system pressure is down to zero 12 In the Chromeleon software consider updating the Predictive Performance information for the pistons 13 Optional Test the system wash system and pump for leakage see Testing the Seal Wash System for Leakage page 130 and Testing the Pump for Leakage General Test page 179 14 Reco...

Page 146: ...ht makes dirt particles appear larger 2 Recommended Even if the piston seems to be clean perform the fingernail test a Hold the piston and carefully move your fingernail over the piston surface The piston is clean when the fingernail test does not reveal inconsistent or rough areas b Continue with the next step 3 Carefully rinse the piston preferably using isopropanol 4 With a dry lint free tissue...

Page 147: ...ot a wear part You need not replace the support ring when you replace a piston seal Replace the support ring only when applicable Seal wash seals and seal handling tool NOTICE All pumps except VC P21 and VC P33 Whenever you remove the pump head you must replace the seal wash seals Tools and additional items required Syringe filled with isopropanol Cleaning swab Lint free tissue Seal handling tool ...

Page 148: ...eal wash plate 3 Seal wash bodies 4 Support ring 2 Remove the seal wash bodies from the pump head 3 Remove the support ring Gently tap the pump head on a clean work surface to make the support ring drop out If the support ring does not drop out use the seal handling tool With the insertion pin push the seal handling tool at a slight angle into the pump head and remove the tool The support ring is ...

Page 149: ...om the inside and outside using isopropanol a cleaning swab and a lint free tissue 6 Pipette a few drops of isopropanol into cavity in the pump head on the edge on which the piston seal will rest Figure 44 Pump head left no piston seal installed right piston seal installed No Description 1 Edge on which the piston seal will rest 2 Piston seal installed 7 Slide the support ring onto the insertion p...

Page 150: ...installed 11 Insert the seal wash bodies into the pump head Mind the correct orientation The even side faces the pump head Figure 47 Orientation of the seal wash body No Description 1 Side facing the seal wash plate 2 Side facing the pump head even side 12 Replace the seal wash seals Follow the appropriate steps in Replacing the Seal Wash Seals page 154 13 Install the pistons Follow the related st...

Page 151: ... the pump specific Performance Qualification tests PQ tests Flow Precision Test and Gradient Accuracy Test For details refer to the Operational and Performance Qualifications manual When the tests are completed execute the QualificationDone command 7 6 6 Recommendations for New Piston Seals When When new piston seals have been installed TIP The actions recommended in this section directly influenc...

Page 152: ...anol 7 Close the purge valve by turning the valve knob clockwise 8 Generate the necessary backpressure For 30 minutes apply the flow indicated in the table using isopropanol Pump Type Flow Backpressure VC pumps 1 mL min approximately 45 MPa approximately VF pumps 2 mL min approximately 80 MPa approximately 9 Turn off the pump flow and wait until the system pressure is down to zero 10 Remove the ca...

Page 153: ...e tubing for a longer period limits the tubing lifetime 1 To absorb any liquid that might be leaving the purge outlet when the tubing is removed place the tissue under the outlet 2 On the purge sensor unit remove the silicone tubing from the purge outlet The other end of the tubing remains in the purge drain port outlet 3 Hold the tubing so that any liquid that is present in the tubing flows off t...

Page 154: ...er you removed the pump head Once a year Parts required Seal wash seals Tools and additional items required Syringe filled with isopropanol Seal handling tool Figure 48 Seal handling tool No Description 1 Seal removal pin 2 Seal insertion pin Preparations 1 Remove the pump head see Replacing the Pump Head page 138 2 Remove the pistons see Replacing the Pistons page 144 ...

Page 155: ...seal handling tool at a slight angle to the seal Push the seal out of the plate and remove the tool Figure 50 Removing the seal wash seal 3 Slide the new seal onto the insertion pin of the seal handling tool 4 To facilitate seal installation inject a few drops of isopropanol into the seal cavities on the seal wash plate no channel side 5 On the seal wash plate no channel side set the insertion pin...

Page 156: ...nstall the pistons Follow the related steps in Replacing the Pistons page 144 9 Install the pump head See Installing the Pump Head page 140 10 Inspect all flow connections on the pump head for indications of leakage Tighten leaky connections when the system pressure is down to zero 11 Test the system wash system and pump for leakage see Testing the Seal Wash System for Leakage page 130 and Testing...

Page 157: ... PQ tests Flow Precision Test and Gradient Accuracy Test For details refer to the Operational and Performance Qualifications manual When the tests are completed execute the QualificationDone command 7 6 8 Replacing the Pump Head Seals When Damage to pump head seals leaky seals Parts and tools required Pump head seals Seal wash seals and seal handling tool NOTICE All pumps except VC P21 and VC P33 ...

Page 158: ...oove shape until the seal rests exactly in the groove 5 Insert the seal wash bodies into the pump head Mind the correct orientation The even side faces the pump head Figure 54 Orientation of the seal wash body No Description 1 Side facing the seal wash plate 2 Side facing the pump head even side 6 Replace the seal wash seals see Replacing the Seal Wash Seals page 154 7 Install the pistons Follow t...

Page 159: ...lsation shift in retention times and or poor reproducibility can indicate possible piston seal leakage If messages regarding piston seal leakage appear in the chromatography software for example The piston seal leakage has exceeded the recommended limit code 4121 Parts and additional items required Backpressure capillary from Diagnostics tool kit optional Isopropanol Follow these steps 1 From the ...

Page 160: ... tightly Reconnect the seal wash tubing to the seal wash detector port labeled in This completes the test Liquid level in the tubing is decreasing The seal wash seals are leaky 1 Replace the seal wash seals 2 Reconnect the seal wash tubing to the seal wash detector port labeled in 3 Repeat the test starting with step 1 Liquid level in the tubing is increasing One or more piston seals are leaky Iso...

Page 161: ... Reconnect the seal wash tubing to the seal wash detector port labeled in 4 Repeat the test starting with step 1 Liquid level in the tubing does not increase again 1 Replace the piston seals in the right pump head 2 Reconnect the seal wash tubing to the right pump head 3 Reconnect the seal wash tubing to the seal wash detector port labeled in 4 Repeat the test starting with step 1 TIP If you obser...

Page 162: ... see the next section To replace the outlet check valves see Replacing the Outlet Check Valves page 164 To clean the check valves see Cleaning the Check Valves page 167 7 7 1 Replacing the Inlet Check Valves The picture shows the parts that must be removed Figure 56 Replacing the inlet check valve No Description 1 Tubing on inlet check valve 2 Inlet check valve including 2a Valve nut 2b Valve cart...

Page 163: ...ppropriate solvent 2 Turn off the pump flow and wait until the system pressure is down to zero Follow these steps 1 On the valve nut disconnect the tubing 2 Use the open end wrench to loosen and remove the valve nut from the pump head 3 Remove the valve cartridge Valve Cartridge Action Valve cartridge removed together with valve nut Hold the nut over your hand and turn it upside down to allow the ...

Page 164: ... from entering the Vanquish system 8 Inspect the inlet check valve and fitting connection for indications of leakage Tighten leaky connections when the system pressure is down to zero 9 Optional Test the pump for leakage see Testing the Pump for Leakage General Test page 179 7 7 2 Replacing the Outlet Check Valves The picture shows the parts that must be removed Figure 57 Replacing an outlet check...

Page 165: ...ventional capillary non Viper Open end wrench size 1 4 Preparations 1 To flush out harmful substances purge the pump with an appropriate solvent 2 Turn off the pump flow and wait until the system pressure is down to zero Follow these steps 1 Remove the capillary that connects the pump head to the purge sensor unit Disconnect the capillary on both the pump head outlet and purge sensor unit 2 Remove...

Page 166: ...llary that connects the pump head to the purge sensor unit Observe the following Install the capillary in the direction of solvent flow Note that one end of the capillary is longer than the other end Connect the longer end to the pump head 9 Rinse the pump thoroughly Use the solvent for your application Open the purge valve to prevent the rinsing liquid from entering the Vanquish system 10 Inspect...

Page 167: ...eps in Replacing the Inlet Check Valves page 162 or Replacing the Outlet Check Valves page 164 as applicable Follow these steps 1 In the direction of flow fill the check valves with isopropanol using the syringe 2 For 10 minutes place the check valves in the ultrasonic bath 3 In the direction of flow rinse the check valves with isopropanol using the syringe 4 Reinstall the check valves following t...

Page 168: ...c Mixer for Permeability TIP With the dual pump test each static mixer separately When If you observe pressure pulsation inconstant pressure or high backpressure at the column and pump Additional items required Water as solvent Tissue Follow these steps NOTICE Turning the static mixer in the mounting bracket when loosening or tightening capillaries can damage the mounting bracket To prevent the mi...

Page 169: ...y of the static mixer Damage of static mixer Leaky capillary mixer Parts tools and additional items required As applicable Static mixer Capillary mixer Depending on the capillary connection Viper capillary Viper knurl usually present on the capillary Conventional capillary non Viper Open end wrench size 1 4 Solvent suitable for purging Preparations 1 To flush out harmful substances purge the pump ...

Page 170: ... mixer from the pump 5 Disconnect the capillary mixer from the static mixer 6 Connect the capillary mixer to the static mixer Mind the direction of flow through the static mixer and capillary mixer indicated by the arrow on the mixer 7 Insert the assembly of static mixer and capillary mixer into the pump 8 Insert the static mixer into the mounting bracket 9 Connect the capillary mixer on the purge...

Page 171: ...175 7 9 1 Emptying the Solvent Lines When To empty the solvent lines for example for maintenance or service procedures or for long term shutdown Parts required Silicone tubing Syringe Preparations 1 Turn off the pump flow and wait until the system pressure is down to zero 2 Prepare the pump for purging see Purging the Pump page 103 Follow these steps Follow these steps for each solvent line that y...

Page 172: ...ump The pump inlet is the vacuum degasser For the pump specific operating principles that illustrate the solvent flow through the pump see Pump Specific Information page 215 Solvent line from the solvent reservoir to the pump inlet Follow the steps Solvent Lines From Reservoir To Pump Inlet page 173 Depending on the pump type Solvent line from the pump inlet to the proportioning valve or degasser ...

Page 173: ... cap 2 Disconnect the solvent line on the pump inlet 3 Remove the solvent line from the pump and all modules above the pump in the system stack When removing the solvent line from the tubing guides be careful not to pull on other tubing in the guides 4 To install the new solvent line follow the related steps in Connecting the Solvent Lines page 64 5 To remove any air bubbles from the solvent lines...

Page 174: ...To remove any air bubbles from the solvent lines purge the pump 7 9 2 3 Solvent Line From Proportioning Valve or Degasser To Pump Head This section applies to all pumps except the isocratic pump When Damage or blockage of the solvent line Parts and additional items required Solvent line between proportioning valve or degasser and pump head inlet as applicable for the pump Solvent suitable for purg...

Page 175: ...lter filter holder bottom part 2 Filter frit 3 Solvent line filter filter holder top part As applicable Filter holder Filter frit Solvent suitable for purging Preparations 1 Turn off the pump flow and wait until the system pressure is down to zero Follow these steps 1 Unscrew the cap of the solvent reservoir and remove the cap together with the solvent line from the reservoir 2 Remove the filter h...

Page 176: ...at the frit is in a level position c Screw the filter top to the filter bottom 5 Slide the filter holder onto the solvent line 6 Retighten the reservoir cap hand tight Verify that the retaining guide remains in the hole in the reservoir cap If it does not press the retaining guide into the hole to ensure that the tubing is kept in place in the cap 7 To remove any air bubbles from the solvent lines...

Page 177: ...ge valve knob with integrated seals No Description 1 Purge valve knob 2 Ring seal 3 Cap seal NOTICE Avoid holding the new valve knob by the seal end Scratches on the seals will prevent a proper seal and cause leakage Additional items required Solvent suitable for purging to flush out harmful substances Cleaning swab Solvent suitable for your application Preparations 1 To flush out harmful substanc...

Page 178: ...ng the new purge valve knob clean the opening in the purge sensor unit with a cleaning swab 3 Insert the new purge valve knob into the purge sensor unit 4 To tighten turn the valve knob clockwise with your fingers until it is finger tight NOTICE Turn the valve knob only with your fingers Overtightening may damage the purge valve seals 5 Resume operation 6 Inspect the purge outlet If liquid leaves ...

Page 179: ...ose the pump outlet with the fitting plug 3 In the Chromeleon software set the upper pressure limit to the appropriate value Pump Upper Pressure Limit VC pump 60 MPa VF pump 80 MPa 4 Apply a flow of 50 µL min and use the solvent for the application 5 Observe the pressure When the pressure reading is as indicated in the table change the flow rate to a lower value Pump Pressure VC pump Between 30 MP...

Page 180: ...plication or water operate the pump for 2 hours or longer at 35 MPa Check valves If leakage is observed on the connection port turn off the pump flow When the system pressure is down to zero tighten the check valve a little more Remove the valve cartridge Clean the cartridge in an ultrasonic bath If cleaning is not sufficient replace the cartridge Purge valve knob Inspect the purge valve knob and ...

Page 181: ...g Manual Page 181 5 Monitor if the pressure drops at the same rate as before If the pressure drops significantly slower the connection was leaky 6 When testing the pump for leakage is complete reset the upper pressure limit to the value used before the leakage test ...

Page 182: ...present inside the device that could cause an electric shock or damage to the device Turn off the device with its main power switch Disconnect the power cord from both the power source and the device Use only the fuses of the type and current rating specified for the device by Thermo Fisher Scientific Do not use repaired fuses and do not short circuit the fuse holders Follow these steps The fuse h...

Page 183: ... the screwdriver to remove the fuse holder 2 Replace the two fuses with new fuses of the specified type and current rating Always replace both fuses 3 Reinstall the fuse holder 4 Reconnect the power cord to the power source and to the device 5 Turn on the device with the main power switch ...

Page 184: ...rument that includes the device 4 Wait until the Instrument is idle 5 Turn off the pump flow and wait until the system pressure is down to zero Follow these steps 1 Start the Instrument Configuration Manager program 2 Perform a firmware update from the General tab page in the configuration dialog box for the device For details refer to the Chromeleon Help The firmware update may take several minut...

Page 185: ... the firmware update was successful or failed If the firmware update failed turn the device off and on again and repeat the firmware update If the firmware update fails repeatedly contact Thermo Fisher Scientific Technical Support for assistance 4 After a successful firmware update requalification of the device may be required See the release notes for a recommendation ...

Page 186: ...ection Parts required Replacement door Follow these steps NOTICE To avoid damage to the door hinges be careful when performing the following sequence of steps and do not apply force 1 To remove a door push the door upward while opening Open the door to a position in which the two hinges on the housing are aligned in the grooves on the door You can remove the door only when the hinges are in the gr...

Page 187: ...d remove the door 3 To install the door align the door with the hinges on the housing Be careful not to clamp tubing or capillaries between the door and the enclosure 4 Insert the hinges in the groove by pushing up and slightly turning the door 5 Push the door downward to lock it in place You can close the door only when it is properly installed ...

Page 188: ...when removing it Use the carrying handles that were shipped with the device to move or transport the device Never move or lift the device by the front doors This will damage the doors or the device Follow these steps 1 Prepare the device for transport See Preparing the Device for Transport page 188 2 The step depends as follows To transport the device to a new location follow the instructions in T...

Page 189: ...m stack see Removing the Slide In Module page 191 7 15 2 Transporting the Device to a New Location Preparations Prepare the device for transport See Preparing the Device for Transport page 188 Follow these steps 1 Observe the notes for handling and lifting the device safely 2 Transport the device to the new location 3 Install and set up the device in the system stack Follow the instructions on mou...

Page 190: ...ubstances may have contaminated the device during operation and may cause personal injury to service personnel Decontaminate all parts of the device that you want to return for repair Fill in and sign the Health and Safety Form Thermo Fisher Scientific refuses to accept devices for repair if the Health and Safety Form is missing incompletely filled in or unsigned Restarting the Device after Shippi...

Page 191: ...o persons A team effort is in particular required when lifting the device into the system stack or when removing it Tools required Screwdriver Torx T20 Preparations 1 Prepare the device for transport See Transporting or Shipping the Device page 188 Follow these steps 1 Loosen the four captive screws on the front left and front right of the device Figure 62 Captive screws on the slide in module 2 P...

Page 192: ...e The following steps require a team effort a Take the slide in module on both sides from below b Pull the slide in module from the rails towards the front c Place the slide in module on a clean and stable surface 7 16 2 Returning the Slide In Module Preparation If not yet done Remove the slide in module from the enclosure See Removing the Slide In Module page 191 Follow these steps 1 Install the ...

Page 193: ...d Bulky Device The device is too heavy or bulky for one person alone to handle safely To avoid personal injury or damage to the device observe the following guidelines Physical handling of the device including lifting or moving requires a team effort of two persons A team effort is in particular required when lifting the device into the system stack or when removing it Tools required Screwdriver T...

Page 194: ...ide in module on both sides from below b Lift the slide in module to the height of the enclosure c Place the slide in module in the enclosure so that the module sits in the enclosure by approximately 25 cm d Push the slide in module onto the rails and into the enclosure until the slide in module sits completely in the enclosure Figure 64 Inserting the slide in module 4 To facilitate tightening the...

Page 195: ...he slide in module a Connect the slide in module and set up flow connections see Installation page 45 b Prepare the slide in module for first time operation see Preparing the Device for Operation page 95 2 Prepare all other modules of the Vanquish system for operation and restart them Refer to the Operating Manuals for the modules 3 Before starting an analysis let the chromatography system equilib...

Page 196: ...7 Maintenance and Service Page 196 Pumps C Pumps F VC Pxx VF Pxx Operating Manual ...

Page 197: ...8 Troubleshooting Pumps C Pumps F VC Pxx VF Pxx Operating Manual Page 197 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the device ...

Page 198: ...isher Scientific Technical Support for assistance See the contact information at the beginning of this manual To facilitate device identification have the serial number and technical name available when communicating with Thermo Fisher Scientific Status Indicators The status indicator LED bar on the front side of the device and the STATUS LED on the keypad inside provide quick visual feedback on t...

Page 199: ... Console Data view in the folder of the Instrument Messages in the Chromeleon Audit Trail are preceded by an icon The icon identifies the seriousness of the problem refer to the Chromeleon Help For possible causes and remedial actions see Messages page 200 Firmware Failure If a firmware failure occurred during operation of the module an exception log has been created about the processes during the...

Page 200: ...ng to the left pump block or components in the left pump head for example the pistons or check valves The Device Names are specified in the dialog box for the pump in the Instrument Configuration Manager The default names are Pump Device Name Default Name Binary pump Isocratic pump Quaternary pump Main Device Name PumpModule Pump Device Name Pump Dual Pump Main Device Name PumpModule Left Pump Dev...

Page 201: ...the leakage reported earlier with code 33 was not resolved within 3 minutes Find and eliminate the source for the leakage see Resolving Liquid Leaks page 209 Code 36 Download failed The firmware download has not been successful Repeat the download Code 37 Download firmware mismatch You tried to download a firmware file that is invalid for the pump Repeat the download with a firmware file that is v...

Page 202: ...r blockage If the flow path is blocked The column may be contaminated Rinse or replace the column If the problem occurs due to column ageing it may be sufficient to increase the setting for the upper pressure limit The autosampler may be blocked Check the autosampler components and eliminate the source for the blockage If the flow path is not blocked Test the static mixer for permeability see Test...

Page 203: ...iled because pressure is out of range The pressure has not been down to zero during the self test Verify that the pressure sensor cables are properly connected 1 Open the purge valve 2 Repeat the self test 3 Close the purge valve If the message appears again the system pressure sensor may be defective Contact Technical Support Code 4114 The device is busy Please retry after 1 minute The pump is no...

Page 204: ... page 131 Verify that the peristaltic tubing is properly inserted in the seal wash pump and that the lever of the pump is not blocked Inspect the electrodes of the seal wash detector for contamination or damage Replace the seal wash detector as necessary see Replacing the Seal Wash Detector page 134 Make sure that the seal wash liquid is sufficiently conductive see Choosing the Seal Wash Liquid pa...

Page 205: ...eps 1 Open the purge valve 2 Dock the pistons 3 Close the purge valve The message alerts you during internal pump maintenance If the message appears during the internal maintenance procedure perform an additional purge cycle Code 4130 Pressure buildup during maintenance operation A pressure of more than 0 5 MPa built up during piston maintenance or piston seal maintenance To reduce the pressure pe...

Page 206: ...nection and flow path for clogging Then perform a self test Follow these steps 1 Verify that the pressure sensor cables are properly connected Reconnect the cables if necessary 2 Check the flow path for clogging and remedy the situation if necessary 3 Perform a self test If the self test is not successful contact Technical Support Code 4234 The flow was stopped due to a request from another module...

Page 207: ...is offline The autosampler is not accessible via a System Interlink connection Check that the autosampler is properly connected via System Interlink and that the autosampler is powered on Code 4243 Unsupported autosampler The autosampler in the system configuration does not support this procedure Code 4244 The column compartment is offline The column compartment in the system configuration is not ...

Page 208: ... Liquid Leaks page 209 Tests The following tests assist you in finding out whether leakage is present in the pump and they help you to locate the source of a leak Pump leak test general test See Testing the Pump for Leakage General Test page 179 Piston seal leak test See Testing the Piston Seals for Leakage page 159 Seal wash system leak test See Testing the Seal Wash System for Leakage page 130 ...

Page 209: ...intenance and Service page 117 Follow these steps 1 Locate the source of the leak As leakage usually occurs at a connection visually inspect all components and connections in the flow path The following tests can help you to identify the source Leakage test for the seal wash system See Testing the Seal Wash System for Leakage page 130 Leakage test for the piston seals See Testing the Piston Seals ...

Page 210: ...high backpressure at the column and pump See Testing the Static Mixer for Permeability page 168 8 5 1 Pressure Not Zero with Open Purge Valve When The pressure is not down to zero when the purge valve is open Follow these steps 1 For the pressure sensor for which the pressure is not down to zero check the pressure sensor cable and connector for proper contact a Unplug the pressure sensor cable b R...

Page 211: ... Action Lower than indicated in the table The outlet check valve may be defective Replace the valve cartridge See Replacing the Outlet Check Valves page 164 Higher than indicated in the table The piston seal may be defective Replace the piston seal See Replacing the Piston Seals or Support Ring page 147 The inlet check valve may be defective Replace the valve cartridge See Replacing the Inlet Chec...

Page 212: ...s to the static mixer in this section apply equally to the inline filter if installed When If you observe pressure pulsation inconstant pressure or high backpressure at the column and pump Additional items required Water as solvent Tissue Follow these steps Test the static mixer by following the steps in Testing the Static Mixer for Permeability page 168 ...

Page 213: ...perating Manual Page 213 8 6 Removing Persistent Air Bubbles If you are unable to remove air bubbles with purging purge the pump against backpressure Follow the procedure for running in new piston seals in Recommendations for New Piston Seals page 151 ...

Page 214: ...8 Troubleshooting Page 214 Pumps C Pumps F VC Pxx VF Pxx Operating Manual ...

Page 215: ...c Information Pumps C Pumps F VC Pxx VF Pxx Operating Manual Page 215 9 Pump Specific Information This chapter provides specific information about the pump for example the interior components and operating principle ...

Page 216: ...Pxx Operating Manual 9 1 Introduction to this Chapter This chapter provides information that is specific to the pumps for example the interior components and the operating principle Quaternary Pump and Dual Pump page 217 Binary Pump page 228 Isocratic Pump page 233 ...

Page 217: ...ormation in this section applies equally to the quaternary pump and the two pumps of the dual pump Quaternary Pump and Dual Pump See Interior components overview see Quaternary Pump page 218 Dual Pump page 219 Operating Principle page 221 Dwell Volume of the Pump page 222 Setting the Solvent Composition page 223 Synchronizing the Injection Time with the Pump Strokes page 224 Dual pump only See Dua...

Page 218: ...ps for routing solvent lines to the proportioning valve 4 Pump head 5 Purge sensor unit including purge valve and system pressure sensor 6 Leak sensor 7 Type label indicating the module name serial number part number and revision number if any 8 Drain port for guiding leaking liquid to waste 9 Seal wash detector drop detector 10 Vacuum degasser 11 Seal wash pump 12 Tubing chase with tubing guides ...

Page 219: ...system pressure sensor left pump 6 Proportioning valve 3 channels right pump 7 Pump head right pump 8 Purge sensor unit including purge valve and system pressure sensor right pump 9 Leak sensor 10 Drain port for guiding leaking liquid to waste 11 Type label indicating the module name serial number part number and revision number if any 12 Seal wash detector drop detector 13a Vacuum degasser Degas ...

Page 220: ... mixer as part of mixing system right pump 17 Pump block LED right pump 18 Pump outlet outlet of the static mixer right pump 19a Capillary mixer as part of mixing system left pump 19b Static mixer as part of mixing system left pump 20 Pump block LED left pump 21 Pump outlet outlet of the static mixer left pump ...

Page 221: ...A D 3 solvents available with the dual pump solvents A C with each of the two pumps 1 Solvent degasser vacuum degasser pump inlet 2 Proportioning valve 3 Pump head with 3a Working cylinder pump head 3b Equilibration cylinder pump head 4 Purge sensor unit with 4a Purge valve knob 4b Purge outlet 4c System pressure sensor 5 Mixing system with 5a Capillary mixer 5b Static mixer 6 Pump outlet outlet o...

Page 222: ...gradient delay volume by the dwell volume which is the calculated volume between the point where the gradient is formed and the pump outlet Figure 68 Dwell volume of the pump No Description A D Solvent options 4 solvents available with the quaternary pump solvents A D 3 solvents available with the dual pump solvents A C with each of the two pumps 1 Solvent degasser vacuum degasser 2 Proportioning ...

Page 223: ...ng if required Number of the solvents to be used with the pump Standard solvent names These solvent names appear in the Chromeleon user interface 2 In the Chromeleon Client determine the percentage of solvent that you want to deliver Set the percentage for solvents B C and D B C D Solvent A A automatically delivers the remaining volume Examples To deliver 100 of solvent A Set B C and D to 0 To del...

Page 224: ...anquish pump The synchronization ensures that all injections are performed at the same phase of the pump cycle Synchronization is possible only when configured in the Chromeleon software refer to the Chromeleon Help Then the following properties are available in the Chromeleon software SyncWithPump To enable synchronization set the property to On To disable synchronization for example for a specif...

Page 225: ...a whole or separately for each pump see Monitoring the Solvent Consumption page 226 You can monitor the liquid level in the waste container of the dual pump as a whole or separately for each pump see Monitoring the Liquid Level in the Waste Container page 227 9 2 6 1 Sharing the Dual Pump In the Chromeleon software you can share the dual pump between two instruments The default assignment for the ...

Page 226: ...mp Monitoring the Solvent Consumption for the Dual Pump as a Whole TIP It is possible to share solvent reservoirs even if the two pumps are assigned to different instruments 1 In the Instrument Configuration Manager open the dialog box for the dual pump 2 On the Bottles tab page select the Share Eluent Bottles check box 3 In the Chromeleon user interface enter the information that is required for ...

Page 227: ... separately for each pump Monitoring the Liquid Level for the Pump as a Whole TIP It is possible to share the waste container even if the two pumps are assigned to different instruments 1 In the Instrument Configuration Manager open the dialog box for the dual pump 2 On the Bottles tab page select the Share Waste Bottles check box 3 In the Chromeleon user interface enter the information that is re...

Page 228: ...user accessible components of the pump are located directly behind the front doors Figure 69 Interior view No Description 1 Keypad with buttons for pump control 2a Pump head left 2b Pump head right 3 Tubing clips for routing the solvent lines from the degasser outlets to the pump heads 4 Purge sensor unit including purge valve and system pressure sensor 5 Leak sensor 6 Drain port 7 Type label indi...

Page 229: ...tion 9b Vacuum degasser solvent A 10a Solvent selector solvent B 10b Solvent selector solvent A 11 Seal wash pump 12 Tubing chase with tubing guides 13 Pump block LEDs 14a Capillary mixer as part of mixing system 14b Static mixer as part of mixing system 15 Pump outlet outlet of the static mixer ...

Page 230: ...uum degasser 3 Pump head left with 3a Working cylinder left pump head 3b Equilibration cylinder left pump head 4 Pump head right with 4a Working cylinder right pump head 4b Equilibration cylinder right pump head 5 Purge sensor unit with 5a Purge valve knob 5b Purge outlet 5c System pressure sensor 6 Mixing system with 6a Capillary mixer 6b Static mixer 7 Pump outlet outlet of the static mixer For ...

Page 231: ...dient Delay Volume Dwell Volume and Ripple page 236 The pump contributes to the system gradient delay volume by the dwell volume which is the calculated volume between the point where the gradient is formed and the pump outlet Figure 71 Dwell volume of the pump No Description 1 Solvent selectors 2 Solvent degasser 3 4 Pump heads 5 Purge sensor unit 6 Mixing system with capillary mixer and static m...

Page 232: ...solvent names These solvent names appear in the Chromeleon user interface 2 In the Chromeleon Client set the solvent composition For each of the two channels select the solvent and set the percentage of solvent that you want to deliver Selecting the solvent 1 For channel A set A_Selector to A1 or A2 or A3 2 For channel B set B_Selector to B1 or B2 or B3 Determining the percentage of solvent to del...

Page 233: ...Description 1 Keypad with buttons for pump control 2 Pump head 3 Purge sensor unit including purge valve and system pressure sensor 4 Leak sensor 5 Type label indicating the module name serial number part number and revision number if any 6 Drain port for guiding leaking liquid to waste 7 Seal wash detector drop detector 8 Seal wash pump 9 Tubing chase with tubing guides 10 Pump block LED 11a Capi...

Page 234: ...ating principle No Description A Solvent 1 Pump head with 1a Working cylinder 1b Equilibration cylinder 2 Purge sensor unit with 2a Purge valve knob 2b Purge outlet 2c System pressure sensor 3 Mixing system serving as inline filter with 3a Capillary mixer 3b Static mixer 4 Pump outlet outlet of the static mixer For additional information see Operating Principle page 33 ...

Page 235: ...for Specific Applications Pumps C Pumps F VC Pxx VF Pxx Operating Manual Page 235 10 Modifying the Pump for Specific Applications This chapter provides information about how you can modify the pump for specific applications ...

Page 236: ...74 Gradient delay volume of an HPLC system No Description 1 Pump with 1a Point where the gradient is formed purge unit Quaternary pump The gradient is formed after the proportioning valve Dual pump The gradient is formed after the proportioning valve with each of the two pumps Binary pump The gradient is formed in the purge sensor unit 2 Autosampler 3 Column 4 Detector 5 Gradient delay volume of t...

Page 237: ...dwell volume of the pump and thus the gradient delay volume of the system For information about the available mixing systems see Available Mixing Systems page 237 10 1 1 Available Mixing Systems Each mixing system comprises a static mixer and a capillary mixer The volumes of both mixers determine the entire volume of the mixing system The table lists the available mixing systems Description Remark...

Page 238: ...µL comprising Static mixer volume 750 µL Capillary mixer volume 50 µL See TIP at the end of this table Mixing system volume 1550 µL comprising Static mixer volume 1500 µL Capillary mixer volume 50 µL See TIP at the end of this table TIP With this mixing system only the static mixer is different from the static mixer with which the pump is shipped The capillary mixer remains the same Thus if you wa...

Page 239: ...t 2 To remove harmful substances from the components in the flow path after the purge unit have the pump deliver for a short time with the solvent that was used for purging 3 Turn off the pump flow and wait until the system pressure is down to zero Follow these steps NOTICE Turning the static mixer in the mounting bracket when loosening or tightening capillaries can damage the mounting bracket To ...

Page 240: ...Open end wrench size 1 4 Solvent suitable for purging Screwdriver Torx T10 required only for static mixers with a volume of 750 µL or 1500 µL Preparations 1 If not yet done Remove the mixing system with which the pump is shipped see Removing the Mixing System page 239 2 Only required if you want to install a static mixer with a volume of 750 µL or 1500 µL These mixers require a different mounting ...

Page 241: ...he pump same position and orientation as the one you removed Binary pump Reinsert the spacer sleeves Figure 76 Installing the mounting bracket No Description 1 Mounting holes for mounting bracket 2 With binary pump only spacer sleeves 3 Mounting bracket 4 Mounting screws Torx Follow these steps NOTICE Turning the static mixer in the mounting bracket when loosening or tightening capillaries can dam...

Page 242: ...lue the leak tests may not provide reliable results 7 Apply the flow rate of your application and have the pump deliver for a short time 8 Inspect the flow connections on the mixer for indications of leakage before resuming operation 9 In the Chromeleon software consider updating the Predictive Performance information for the static mixer MixerChanged command When operating the pump observe the fo...

Page 243: ...y Viper knurl usually present on the capillary Conventional capillary non Viper Open end wrench size 1 4 Solvent suitable for purging Preparations 1 If not yet done Remove the mixing system with which the pump is shipped see Removing the Mixing System page 239 2 For easier installation remove the seal wash detector assembly funnel and detector Grasp and pull the assembly upward by the funnel The d...

Page 244: ...e mixer 3 Turn and insert the assembly of static mixer and capillary mixer into the pump so that the knurl of the capillary mixer rests on the edge of the purge sensor unit Figure 78 100 µL mixing system installed No Description 1 Static mixer 2 Capillary mixer 3 Knurl resting on the purge sensor unit 4 Tighten the capillary mixer on the purge sensor unit 5 Reinstall the seal wash detector assembl...

Page 245: ... delay volume but increases the ripple The maintenance procedures are the same as for the mixing system with which the pump was shipped see Mixing System page 168 10 1 2 4 Installing the 35 µL Inline Filter Set Parts required Set inline filter volume 35 µL comprising an inline filter static filter volume 10 µL and a capillary mixer volume 25 µL Capillary to connect the pump to the autosampler Tool...

Page 246: ...tor including the tubing and cable can remain connected to the funnel Figure 79 Removing the seal wash detector assembly No Description 1 Funnel seal wash detector 2 Seal wash detector 3 Detector cable 4 Seal wash line 3 To attach the new mounting bracket inside the pump above the purge sensor unit a Remove the push rivets that cover the mounting holes while no mounting bracket is installed b Usin...

Page 247: ...the inline filter loosely tighten the capillary mixer on the purge sensor unit so that you can easily access the free end of the capillary 2 Connect the inline filter to the capillary mixer Mind the direction of flow through the inline filter indicated by the arrow on the filter 3 Turn and insert the assembly of inline filter and capillary mixer into the mounting bracket Figure 81 Inline filter wi...

Page 248: ...romeleon software set the CapillaryMixer property to the value indicated on the capillary mixer 10 Apply the flow rate of your application and have the pump deliver for a short time 11 Inspect the flow connections on the mixer for indications of leakage before resuming operation 12 In the Chromeleon software consider updating the Predictive Performance information for the static mixer MixerChanged...

Page 249: ...le Solvents and Additives page 251 10 2 1 Modifying the Pump and Autosampler This section applies to VC pumps and VC autosamplers When To use normal phase NP compatible solvents and additives in a Vanquish Core system Parts required Normal Phase NP kit including NP compatible tubing and seals for use as both piston seals and seal wash seals For ordering information see Optional Accessories page 26...

Page 250: ...acing the Pistons page 144 When replacing the piston seals also install new pistons Changing the piston seals but keeping the pistons may impair the seal life Replace the seal wash seals with the seals from the Normal Phase kit Follow the replacement instructions in Replacing the Seal Wash Seals page 154 3 Test the pump for leakage Testing the Pump for Leakage Follow the instructions in Testing th...

Page 251: ... Long Term Shutdown To interrupt operation for a longer period and restart operation afterward follow the instructions in this section TIP Shutting down the device affects the operation of the system When shutting down the device also observe the shutting down instructions for the other Vanquish system modules and take appropriate action refer to the Operating Manuals for the modules 1 Flush the c...

Page 252: ...ic Mixer for Permeability page 168 Testing the Pump for Leakage Follow the instructions in Testing the Pump for Leakage General Test page 179 With one exception all values in the procedure that are specific for the VC pump equally apply to the modified VC pump Exception When the pressure is 55 MPa apply the flow indicated in the table and proceed with observing the pressure The pressure should inc...

Page 253: ... Pumps C Pumps F VC Pxx VF Pxx Operating Manual Page 253 11 Specifications This chapter provides the physical and performance specifications including information about the materials used in the flow path of the device ...

Page 254: ...0 bar 4350 psi VF pumps 2 103 MPa 20 1034 bar 290 15000 psi With a flow rate of 5 mL min the pressure range decreases linearly down to 80 MPa 800 bar 11600 psi Pulsation 1 0 or 0 2 MPa whichever is greater Gradient formation Low pressure gradient proportioning Proportioning accuracy 0 5 of full scale Proportioning precision 0 15 SD Number of solvents VC pumps VC P20 VC P21 4 VC P32 VC P33 2 x 3 VF...

Page 255: ...Stainless steel titanium ceramics sapphire PEEK UHMW PE fluoropolymers VF pumps MP35N titanium ceramics sapphire PEEK UHMW PE fluoropolymers For information about the chemical resistance of materials refer to the technical literature Solvent and additive information See Solvent and Additive Information page 26 Safety features Leak detection and safe leak handling excess pressure monitoring Good La...

Page 256: ...bar 11600 psi Pulsation 1 0 or 0 2 MPa whichever is greater Gradient formation High pressure gradient proportioning Proportioning accuracy 0 2 of full scale Proportioning precision 0 15 SD Number of solvents 2 out of 6 Maximum stroke volume 80 µL Mixer volume VC pump Standard configuration 400 µL mixing system consisting of a capillary mixer volume 50 µL and a static mixer volume 350 µL Other mixi...

Page 257: ...nium ceramics sapphire PEEK UHMW PE fluoropolymers VF pumps MP35N titanium ceramics sapphire PEEK UHMW PE fluoropolymers For information about the chemical resistance of materials refer to the technical literature Solvent and additive information See Solvent and Additive Information page 26 Safety features Leak detection and safe leak handling excess pressure monitoring Good Laboratory Practice GL...

Page 258: ...volume 50 µL and a static mixer volume 150 µL Other mixing systems inline filters are available as an option Solvent degassing No available as optional accessory Biocompatible No Communication USB 1 USB port USB 2 0 B type connector 1 USB hub with 3 ports USB 2 0 A type connectors I O Interface 2 Dig I O ports mini DIN each providing one input one relay output and one bidirectional input output Sy...

Page 259: ...edictive Performance functions for scheduling maintenance procedures based on the actual operating and usage conditions of the pump All system parameters logged in the Chromeleon Audit Trail Typical operating conditions for measurable specifications Flow accuracy flow precision pulsation 1 mL min at 20 MPa or 2 mL min at 40 MPa water ...

Page 260: ...re 20 C 45 C Ambient operating humidity 20 80 relative humidity non condensing Ambient storage humidity Maximum 60 relative humidity non condensing Operating altitude Maximum 2000 m above sea level Pollution degree 2 Power requirements 100 240 VAC 10 50 60 Hz max 245 W 255 VA Overvoltage category II Emission sound pressure level 70 dB A typically 40 dB A Dimensions height x width x depth 19 2 x 42...

Page 261: ...ng Manual Page 261 12 Accessories Consumables and Replacement Parts This chapter describes the standard accessories that are shipped with the device and the accessories that are available as an option This chapter also provides information for reordering consumables and replacement parts ...

Page 262: ... the replacement parts and additional components options and peripherals specifically authorized and qualified by Thermo Fisher Scientific Accessories consumables and replacement parts are always maintained at the latest technical standard Therefore part numbers are subject to change If not otherwise stated updated parts will be compatible with the parts they replace ...

Page 263: ...mL 1 Tubing connector 90 angled 2 Solvent line filter filter holder no filter frit included 6 Solvent line filter filter frit 10 µm pack of 10 1 Plugs and retaining guides for reservoir caps kit including Cap plug to close open holes in the reservoir cap pack of 5 Retaining guide to keep the liquid line in place in the reservoir cap pack of 2 7 Fitting plug Viper 1 Tool kit including Open end wren...

Page 264: ... in the reservoir cap pack of 5 Retaining guide to keep the liquid line in place in the reservoir cap pack of 2 7 Fitting plug Viper 1 Tool kit including Open end wrench size 11 x 13 mm Double ended ring wrench size 11 x 13 mm Open end wrench size 1 4 x 5 16 Hexagon wrench size 6 Seal handling tool Spacing tool pump head and pistons 1 Installation tool for Viper capillaries with torque toothing 1 ...

Page 265: ... pack of 5 Retaining guide to keep the liquid line in place in the reservoir cap pack of 2 7 Fitting plug Viper 1 Tool kit including Open end wrench size 11 x 13 mm Double ended ring wrench size 11 x 13 mm Open end wrench size 1 4 x 5 16 Hexagon wrench size 6 Seal handling tool Spacing tool pump head and pistons 1 Reservoir 0 25 L with reservoir cap 1 Tubing bracket 1 Solvent lines pack of 6 from ...

Page 266: ... toothing 1 Reservoir 0 25 L with reservoir cap 1 Tubing bracket 1 Solvent lines pack of 6 from solvent reservoirs to pump degasser 1 System interlink cable RJ45 0 5 m 1 USB cable type A to type B high speed USB 2 0 cable length 5 m 1 12 2 3 Isocratic Pump For reordering information of the ship kit components see Consumables and Replacement Parts page 273 VC P40 ship kit Item Quantity in shipment ...

Page 267: ...ing information of the ship kit components see Consumables and Replacement Parts page 273 VC P20 VC P21 ship kit Item Quantity in shipment Silicone tubing 3 m 1 Syringe plastic 12 mL 1 Tubing connector 90 angled 2 Solvent line filter filter holder no filter frit included 4 Solvent line filter filter frit 10 µm pack of 10 1 Plugs and retaining guides for reservoir caps kit including Cap plug to clo...

Page 268: ...compatible 10 µm 4 Plugs and retaining guides for reservoir caps kit including Cap plug to close open holes in the reservoir cap pack of 5 Retaining guide to keep the liquid line in place in the reservoir cap pack of 2 5 Fitting plug Viper 1 Tool kit including Open end wrench size 11 x 13 mm Double ended ring wrench size 11 x 13 mm Open end wrench size 1 4 x 5 16 Hexagon wrench size 6 Seal handlin...

Page 269: ...e containers in real time Monitoring is based on real physical measurement Solvent monitor 4 channel version 6230 1320 Solvent monitor 8 channel version 6230 1310 Buffer Performance kit Description Part No Buffer Performance kit for quaternary or dual pumps To avoid pressure pulsation issues with buffer applications in quaternary pumps or dual pumps Depending on the application two kits may be req...

Page 270: ...line filter See Optional Mixing Systems and Inline Filters page 270 12 3 2 Optional Mixing Systems and Inline Filters Each mixing system comprises a static mixer and a capillary mixer The volumes of both mixers determine the entire volume of the mixing system TIP With mixing systems volumes 200 µL 400 µL 800 µL or 1550 µL only the static mixer is different from the static mixer with which the pump...

Page 271: ...ps 6044 3015 VF pumps 6044 5026 Mixing systems set inline filter VC pumps Description Part No Mixing system volume 100 µL including Static mixer volume 75 µL Capillary mixer volume 25 µL 6045 5100 Set inline filter volume 35 µL including Inline filter static filter volume 10 µL Capillary mixer volume 25 µL In addition order the capillary to connect the pump to the autosampler 6045 3020 Capillary t...

Page 272: ...ion order the capillary to connect the pump to the autosampler 6044 3870 Capillary to connect the pump to the autosampler for use with the 100 µL mixing system and 35 µL inline filter set For use with binary pump isocratic pump and quaternary pump Dual pump The capillaries to use depend on the application workflow and are provided in the respective Workflow Solution Kits For details refer to the d...

Page 273: ...nance kits can include parts that are not required for your specific pump These parts are marked accordingly in the contents lists for the kits VC P10 Description Part No Maintenance kit for VC P10 including Pump head seal 2 PTFE seals 2 each different sized Silicone tubing clear tubing Peristaltic tubing white tubing Tubing connector straight pack of 5 Tubing connector 90 angled Seal wash seal pa...

Page 274: ...of 5 Tubing clip self adhesive pack of 2 Not required with VC P20 Cap seal for purge valve knob without integrated seals Support ring 6040 1951A VC P32 Description Part No Maintenance kit for VC P32 including Pump head seal 2 PTFE seals 2 each different sized Silicone tubing clear tubing Peristaltic tubing white tubing Tubing connector straight pack of 5 Tubing connector 90 angled Seal wash seal p...

Page 275: ...ve knob without integrated seals Support ring 6040 1950A 12 4 1 2 Maintenance Kits VF Pumps TIP Maintenance kits can include parts that are not required for your specific pump These parts are marked accordingly in the contents lists for the kits VF P10 Description Part No Maintenance kit for VF P10 including Pump head seal 2 PTFE seals 2 each different sized Silicone tubing clear tubing Peristalti...

Page 276: ...ng swab pack of 5 Tubing clip self adhesive pack of 2 Not required with VF P20 Cap seal for purge valve knob without integrated seals 6040 1954A VF P32 Description Part No Maintenance kit for VF P32 including Pump head seal 2 PTFE seals 2 each different sized Silicone tubing clear tubing Peristaltic tubing white tubing Tubing connector straight pack of 5 Tubing connector 90 angled Seal wash seal p...

Page 277: ... Identical for inlet check valve and outlet check valve 6041 2301 Pump head seals PTFE O rings sizes 32x1 5 and 9x1 5 5 each 6040 2208 Capillary kit pump head including U tube pack of 2 Capillary from right pump head to purge sensor unit Capillary from left pump head to purge sensor unit 6045 3000 Seal handling tool 6040 7158 VC P20 VC P32 and VC P40 Description Part No Pump head complete assembly...

Page 278: ...e cartridge ceramics Identical for inlet check valve and outlet check valve 6041 2301 Pump head seals PTFE O rings sizes 32x1 5 and 9x1 5 5 each 6040 2208 Capillary kit pump head including U tube pack of 2 Capillary from pump head to purge sensor unit pack of 2 6040 3001 Seal handling tool 6040 7158 12 4 2 2 Pump Heads and Components VF Pumps VF P10 Description Part No Pump head complete assembly ...

Page 279: ...ssembly including spacing tool 6044 5204 Piston seal RP and seal wash seal pack of 2 each 6044 0295 Support ring pack of 2 6040 0012 Piston sapphire pack of 2 6040 0042 Check valve valve nut kit including Nut for inlet check valve Nut for outlet check valve 6037 1964 Check valve cartridge ceramics Identical for inlet check valve and outlet check valve 6041 2301 Pump head seals PTFE O rings sizes 3...

Page 280: ...ash system in the pump and in the autosampler Needle wash system in the autosampler Drain pump in the autosampler NOTICE Use the thick silicone tubing and related tubing connectors in the autosampler Use the thin silicone tubing and related tubing connectors in the pump Use the screwable connectors for the pump head The screwable tubing connectors are not used with the VC pumps and VF pumps NOTE F...

Page 281: ... pump Solvent lines to connect the solvent reservoirs to the degasser inlet ports 6036 1704 Binary pump Solvent lines to connect the solvent reservoirs to the solvent selectors 6036 1701 Isocratic pump Solvent line to connect the solvent reservoir to the pump head 6036 1705 Solvent lines to connect the pump inlet to the pump heads Quaternary pump Tubing kit including Solvent lines from degasser to...

Page 282: ...pumps 6044 5110 Capillary mixers Description Part No Capillary mixer volume 50 µL for use with static mixers volumes 150 µL 350 µL 750 µL and 1500 µL for VC pumps 6044 3015 VF pumps 6044 5026 12 4 5 Miscellaneous Parts Description Part No Capillary nanoViper length 750 mm inner diameter 75 µm To be used for example when running in new piston seals 6041 5780 Cleaning swabs pack of 25 6040 0007 Diag...

Page 283: ...laries and tubing for special applications refer to the related application documentation 12 4 6 Interface Cables and Power Cords Interface cables Description Part No Digital I O signaling cable 6 pin cable length 5 m 6036 0006 System interlink cable 6036 0004 USB cable type A to type B high speed USB 2 0 Cable length 1 m 6035 9035 USB cable type A to type B high speed USB 2 0 Cable length 5 m 691...

Page 284: ...12 Accessories Consumables and Replacement Parts Page 284 Pumps C Pumps F VC Pxx VF Pxx Operating Manual ...

Page 285: ...13 Appendix Pumps C Pumps F VC Pxx VF Pxx Operating Manual Page 285 13 Appendix This chapter provides additional information about compliance and the use of the digital I O ports ...

Page 286: ...azardous substances in electrical and electronic equipment The CE mark on the device indicates that the product is compliant with the directive China RoHS regulations Measures for Administration of the Pollution Control of Electronic Information Products One of the following logos may be present on the device if applicable Logo Description The green logo marks items that do not contain the hazardo...

Page 287: ...its are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause har...

Page 288: ...ff the connection is between COM and NC If the relay is activated the connection is between COM and NO 6 Relay output Relay_NO Normally Open contact The next table lists the functions assigned to the connector pins and the color of the cable wire connected to each pin Pin Wire Color Signal Name Signal Level Remarks 1 Pink Input output Input low active On 0 0 4 V Off 2 2 5 V Open Collector Output 0...

Page 289: ...ted to the external device with the digital I O signaling cable part no 6036 0006 The inputs and outputs that you want to use must be selected in the Instrument Configuration Manager Connecting a Digital I O Port 1 Plug the 6 pin connector of the cable into the digital I O port that you want to use 2 For each relay output or digital input to be used connect the appropriate signal wire and ground w...

Page 290: ...13 Appendix Page 290 Pumps C Pumps F VC Pxx VF Pxx Operating Manual ...

Page 291: ...e setup 82 module setup 82 Predictive Performance 127 Smart Shutdown 113 Smart Standby 113 Smart Startup 96 clean pump 125 cleaning 126 compression 101 compression values 210 condensation 50 55 connectors 53 consumables 261 273 cTUVus mark 286 curve 101 D decontamination 126 degasser mode 101 delivery 44 device optimization 110 preparing for operation 95 restart after long term shutdown 116 shutdo...

Page 292: ...ance 122 isocratic pump 233 K keypad 86 L leak detection 38 101 leak sensor 209 leak sensor mode 101 leak test piston seals 159 209 pump 179 209 pump NP 250 252 purge valve knob 209 seal wash system 130 209 leakage 209 LED bar 86 88 198 liquid leaks 209 liquid level monitoring 99 long term shutdown 114 long term shutdown NP 251 M main power fuses 182 maintenance 117 122 cleaning 126 decontaminatin...

Page 293: ...lothing 22 Pump 224 pump flush 125 pump block LED 86 89 198 pump head maintenance 136 parts 137 pump head seals 157 replace 138 pump head bushing 137 pump head seals replace 157 pump inlet 221 230 pump outlet 218 220 221 229 230 233 234 pump pressure record 98 purge 101 automatic 104 changing solvents 105 empty solvent lines 105 first time operation 105 manual 105 remove air bubbles 105 Purge butt...

Page 294: ...n 50 55 power considerations 49 50 power cord 50 slide in module 191 install 193 remove 191 return 192 Smart Shutdown 113 Smart Standby 113 Smart Startup 96 solvent chloride concentration 27 information 26 pH range 27 use 92 solvent composition 99 solvent consumption 99 226 solvent line filter 65 171 assemble 66 176 replace 175 solvent lines 64 171 connect 64 empty 171 replace 172 shut off valves ...

Page 295: ... 210 retention time shift 210 tubing 59 tubing bracket 57 tubing chase 57 tubing guides 57 type label 19 U Universal Serial Bus 53 54 unpack 42 USB 53 54 V Viper knurl 59 torque toothing 59 W waste 64 waste container share 227 waste level 227 waste level monitoring 100 WEEE 287 ...

Page 296: ...Thermo Fisher Scientific Inc 168 Third Avenue Waltham Massachusetts 02451 USA www thermofisher com ...

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