Thermo Scientific thermoscientific Vanquish Pumps C, Operating Manual

Page: 124 / 296

7 • Maintenance and Service
Page 124 Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual
7.4.3 Flushing the Pump
When
• To avoid contamination of the pump
• With persistent contamination
7.4.3.1 Flushing to Avoid Contamination
When
To avoid contamination of the degassing channels
Items required
Fresh solvent
Observe the following guidelines
The following guidelines refer to conventional use of the Vanquish
system as recommended in this manual, such as using fresh high-quality
solvents (minimum UHPLC-grade or LC/MS-grade) that are replaced
frequently:
• Regularly flushing the degassing channels with fresh solvent is
usually sufficient.
• If you use water, acetonitrile or methanol for your chromatography,
flushing the channels once a week is an appropriate interval.
• Mobile phases containing salts, buffering agents and/or other
additives are inherently prone to organic contamination and/or
biodegradation. They may require a much shorter flushing interval.
• Adapt the interval to the solvents in use.
TIP With persistent contamination, for example, if ghost peaks appear
reproducibly in your blank chromatogram without injecting a sample,
see
Flushing with Persistent Contamination Only (
}
page 125) .