4
Operation
Temperature Control
Thermo Scientific
Sorvall RC 3BP Plus
4-7
3. Prepare the rotor according to the rotor manual.
4. Open the chamber door and install the rotor. Make sure that the rotor is fully seated and locked to
the drive spindle. Install the rotor cover, then close the chamber door.
5. If using logging software, make sure it is running, then press
key.
The rotor will accelerate according to the recalled parameters. After the run the message
END
will
appear in the SPEED display and remain there until the door is opened or the
key will be pressed.
Temperature Control
The centrifuge controls calculate sample temperature during a run. The software assumes that the
sample and the rotor are at SET temperature at the start of the run. If temperatures are not
equilibrated, adjustments made by the centrifuge's control system may not be appropriate Over time,
temperature control should come into range.
Either store the rotor in a controlled temperature environment (such as refrigerator or cold room), or
precool/preheat the rotor in the centrifuge chamber, until the rotor is the same temperature as the
sample and the required SET temperature.
When the centrifuge is used to run large volumes of sample for short amounts of time the temperature
control is less important. During a very short run it is difficult to significantly change sample
temperature.
Rotor Temperature Equilibration
To equilibrate the rotor temperature in the centrifuge chamber:
1. Install the rotor in the rotor chamber, place empty buckets (if applicable) in all positions, and the
rotor cover.
2. Close the centrifuge door.
3. Turn off all options (access with
key).
4. Check to be sure the correct rotor name is in the ROTOR field of the SET DISPLAY. If not, press
the
key until it is.
5. Press the
key.
If cooling, input 1500 rpm.
Note
Rotor names are saved within the program. Make sure that the SET rotor identifies the
installed rotor. Be sure that the installed rotor is correct for the recalled program.
Note
If programs are used within a step run, that have not been defined before, a warning message
will be displayed.
Note
When temperature control is critical, the sample, rotor wtih accessory amd rotor chamber
should all be at SET temperature when
key is presssed. Remove the sample soon after a run has
ended. The temperature in the rotor chamber drops slightly when the rotor is no longer generating
heat due to air friction.
Summary of Contents for RC 3BP Plus
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