PowerEase
®
300W Power Supply User Guide
7
1B
Methods
12B
Getting started
31B
Install the
PowerEase
®
300W
Power Supply
1.
Check the label located at the bottom of the power supply to ensure that the unit is compatible
with locally provided voltage.
2.
Place the PowerEase
®
300W Power Supply on a level laboratory bench. Keep the area around
the power supply clear to ensure proper ventilation of the unit.
3.
For your safety: Position the unit properly such that the On-Off switch and the AC inlet
located on the rear of the unit are easily accessible.
4.
Ensure the AC power switch is in the Off position.
5.
Attach the power cord to the AC inlet. Use only properly grounded AC outlets and power
cords.
6.
Connect the leads from the electrophoresis unit; insert the positive lead (+) into the positive
(red) output jack, and the negative lead (–) into the negative (black) output jack.
13B
Important guidelines
32B
Introduction
The important guidelines for operating the PowerEase
®
300W Power Supply are provided in this
section. We recommend that you carefully review these guidelines before operating the instrument.
Important:
For best results, do
NOT
use the PowerEase
®
300W Power Supply at its maximum
electrical load limits. Variations in buffer conditions can result in exceeding the power supply’s
maximum voltage, current, or power output capacity and produce undesirable variations in
performance.
33B
Recommendation
The duration of electrophoresis can be defined in time (hours/minutes). When using this or any
electrophoresis product, we recommend that you adhere to the protocols given in the
electrophoresis product manuals, and durations, specified in time.
Important:
For best results, follow these important guidelines when running multiple gels and
electrophoresis units concurrently. For example:
Avoid running samples of widely differing salt concentrations or sample buffers at the same
time or on the same gel.
Properly prepare your samples.
Note:
Variations in conductivity due to differences in buffer salt concentrations can affect the run
of all the samples run at the same time.
Note:
For optimal performance, use Constant Voltage Mode when using the
PowerEase
®
300W Power Supply with 1 – 2 protein gels or blots.
14B
Operation modes
34B
Introduction
The PowerEase
®
300W Power Supply is designed to operate under different modes,
Constant
Mode
or
Program Mode
, designed to meet most electrophoresis needs. Use the
Constant
Voltage
,
Constant Power
or
Constant Current Operation
for applications that require only one
specific voltage limit, current limit, or power limit continuously during the entire duration of
electrophoresis.