Neurobasal
™
Medium and Neurobasal
™
‑
A Medium
Catalog Numbers
21103049, 10888022, 12348017, and 12349015
Pub. No.
MAN0007306
Rev.
2.0
WARNING!
Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear,
clothing, and gloves. Safety Data Sheets (SDSs) are available from thermofisher.com/support.
Product description
Neurobasal
™
Medium and Neurobasal
™
‑A Medium are basal media that, when supplemented with B‑27
™
Supplement, meet the special cell
culture requirements of pre‑natal/embryonic and post‑natal/adult brain neuronal cells, respectively. Both Neurobasal
™
Medium and
Neurobasal
™
‑A Medium can be used to cultivate neuronal cells from hippocampus, cortex and other regions of the brain. Both media when
supplemented with B‑27
™
Supplement have demonstrated optimal viability for both long and short term maintenance of homogeneous
populations (<0.5% Glial cells) of neuronal cells without the need for an astrocyte feeder layer. Neurobasal
™
Medium Minus Phenol Red
and Neurobasal
™
‑A Medium Minus Phenol Red are provided for receptor studies such as estrogenic receptors, downstream protein
purification studies or other processes where the presence of phenol red is undesirable.
Contents and storage
Contents
Cat. No.
Amount
Storage
Shelf life
[1]
Neurobasal
™
Medium
21103049
500 mL
2–8°C; Protect from light
12 months
Neurobasal
™
Medium Minus Phenol Red
12348017
500 mL
Neurobasal
™
‑
A Medium
10888022
500 mL
Neurobasal
™
-A Medium Minus Phenol Red
12349015
500 mL
[1]
Shelf life duration is determined from Date of Manufacture.
Procedural guidelines
• Neurobasal
™
Medium or Neurobasal
™
‑A Medium, when
supplemented with B‑27
™
Supplement, contain anti‑oxidants
to reduce reactive oxygen damage and they do not contain
the excitatory amino acids, glutamate and aspartate, making
them amenable to the study of these neurotransmitters.
• Neurobasal
™
‑A Medium, when supplemented with B‑27
™
Supplement, is effective for the growth of tumor cell lines of
neuronal origin.
Culture conditions
Media: Complete Neurobasal
™
Medium or Neurobasal
™
‑A
Medium
Culture type: Adherent
Culture vessels: Multiwell plate or T‑flasks
Temperature range: 36°C to 38°C
Incubator atmosphere: Humidified atmosphere of 5% CO
2
in air.
Ensure proper gas exchange and minimize exposure of cultures to
light.
Prepare complete media
1.
Aseptically add supplements to 100 mL Neurobasal
™
Medium or Neurobasal
™
‑A Medium according to one of the
following conditions.
• Add 2 mL B‑27
™
Supplement or other B‑27
™
Supplement
variants and 0.5 mM L‑glutamine or GlutaMAX
™
Supplement.
• Add 1 mL N‑2 Supplement (100X) and 0.5–
2 mM L‑glutamine or GlutaMAX
™
Supplement.
• Add 1 mL G‑5 Supplement (100X) and 0.5–
2 mM L‑glutamine or GlutaMAX
™
Supplement.
2.
Prior to initial plating of primary hippocampal neurons,
further supplement Neurobasal
™
Medium with 25 µM
(3.7 µg/mL) glutamate.
Some cell lines may require an initial attachment in
2% serum‑supplemented complete Neurobasal
™
Medium.
Once supplemented, the complete Neurobasal
™
Medium is
stable for up to one week when stored in the dark at 2°C to
8°C.
USER GUIDE
For Research Use Only. Not for use in diagnostic procedures.
