Thermo Scientific NanoDrop One, User Manual

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5
Measure using a Custom Factor
Thermo Scientific NanoDrop One User Guide 39
Settings for Nucleic Acid Measurements using a Custom Factor
To show the Custom Factor settings, tap > Custom Factor Setup.
Related Topics
• Instrument Settings
Detection Limits for Nucleic Acid Measurements using a Custom Factor
The lower detection limits and reproducibility specifications for nucleic acids are provided
here . The upper detection limits are dependent on the upper absorbance limit of the
instrument and the user-defined extinction coefficients.
To calculate upper detection limits for nucleic acid samples
To calculate upper detection limits in ng/μL, use the following equation:
(upper absorbance limit
instrumen
t
* extinction coefficient
sample
)
Setting Available Options Description
Custom Factor Enter an integer value
between 15 ng-cm/μL
and 150 ng-cm/μL Constant used to calculate nucleic acid concentration in
modified Beer’s Law equation . Based on extinction coefficient
and pathlength:
f = 1/(

260
* b))
where:
f = factor

= molar extinction coefficient at 260 nm in ng-cm/μL
b = sample pathlength in cm (1 cm for nucleic acids measured
with the NanoDrop One instruments)
Baseline Correction On or off
Enter baseline correction
wavelength in nm or use
default value (340 nm)
Optional user-defined baseline correction . Can be used to
correct for any offset caused by light scattering particulates by
subtracting measured absorbance at specified baseline correction
wavelength from absorbance values at all wavelengths in sample
spectrum. As a result, absorbance of sample spectrum is zero at
specified baseline correction wavelength.