Load the Countess
™
Reusable Slide
1.
Before loading your sample into the Countess
™
Reusable Slide, place a cover slip on the counting
chamber, making sure the cover slip is clean and free of grease.
2.
Gently pipet 10 µL of the sample into the sample inlet, allowing capillary action to draw the sample
into the counting chamber. A properly loaded counting chamber should have a thin, even film of
fluid under the cover slip.
Note:
Each chamber in the Countess
™
Cell Counting Chamber Slide or the Countess
™
Reusable
Slide has a 10
‑
µL sample capacity. Do not overfill the slide chambers.
3.
Load the reusable glass slide into the reusable slide holder.
4.
Insert the reusable slide holder containing the slide into the slide port and gently push into the
instrument until it clicks into place.
5.
Use the touchpad to run the count.
6.
To remove the reusable slide holder, push the slide gently into the instrument until it clicks and a
spring pushes the slide out. Grasp the slide and pull it out the rest of the way.
Chapter 4
Slide preparation and operation
Load the Countess
™
Reusable Slide
4
20
Countess
™
3 Automated Cell Counter User Guide
Summary of Contents for Invitrogen AMQAX2000
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