Thermo Scientific Invitrogen AMQAX2000, User Manual

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Template functions
The Actions screen allows you to edit, import, export, and delete templates. Edit can be used to modify
the currently selected template, while Import, Export, and Delete can be used on multiple templates.
Count parameters
Count parameters are adjusted in the Edit template screen using the Gating sliders. Moving the top
and bottom gate boundaries adjusts the range of size, brightness, and circularity parameters.
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1
Size Gating : Moving the top gate down removes larger objects; moving it up includes larger objects. Moving the
bottom gate up removes smaller objects; moving it down includes smaller objects.
2
Brightness Gating : Moving the top gate down removes brighter objects; moving it up includes brighter objects.
Moving the bottom gate up removes dimmer objects; moving it down includes dimmer objects.
3
Cicularity Gating : Moving the top gate down removes more circular objects; moving it up includes more circular
objects. Moving the bottom gate up removes less circular objects; moving it down includes less circular objects.
4
Gating Boundary Sliders
Live or Dead can be selected only on a brightfield count. For a fluorescence count, select the individual
channels.
Chapter 3
Templates
Template functions
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Countess ™ 3 Automated Cell Counter User Guide
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