Thermo Scientific Heraeus Multifuge X3 F Instructions For Use Manual Download Page 1

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Thermo Scientific

Heraeus Multifuge X

3 F

Heraeus

Multifuge X

3 F

Instructions for Use

Visit us online to register your warranty:

thermofisher.com/labwarranty

50117505-

• 0

/ 2020

Summary of Contents for Heraeus Multifuge X3 F

Page 1: ...Thermo Scientific Heraeus Multifuge X3 F Heraeus Multifuge X3 FR Instructions for Use Visit us online to register your warranty thermofisher com labwarranty 50117505 e 03 2020 ...

Page 2: ...WEEE Conformity This product is subject to the regulations of the EU Waste Electrical Electronic Equipment WEEE Directive 2012 19 EU It is marked by the following symbol ...

Page 3: ...n 1 6 Chapter 2 Before use 2 1 Before Setting up 2 2 Setting up 2 2 Transporting the Centrifuge 2 4 Location 2 4 Aligning the Centrifuge 2 4 Mains Connection 2 5 Storage 2 6 Chapter 3 Control Panel 3 1 Control Panel 3 2 Keys 3 2 Chapter 4 Operation 4 1 Switch on Centrifuge 4 2 Lid Opening 4 2 Close Lid 4 2 Rotor Installation 4 2 Entering Parameters 4 4 Centrifugation 4 10 Temperature Adaptation du...

Page 4: ...nce and Care 6 1 Cleaning Intervalls 6 2 Cleaning 6 2 Disinfection 6 3 Decontamination 6 4 Autoclaving 6 5 Service of Thermo Fisher Scientific 6 5 Chapter 7 Trouble Shooting 7 1 Mechanical Emergency Door Release 7 2 Troubleshooting by User 7 3 When to contact Customer Service 7 5 Chemical Compatibility Chart A 1 ...

Page 5: ...ifuge is an IVD accessory and therefore subject to the Directive 98 79 EC of the European Parliament and the Council of October 27 1998 on in vitro diagnostic medical devices This centrifuge is a laboratory product designed to separate components by generation of Relative Centrifugal Force It separates human samples e g blood urine and other body fluids collected in appropriate containers either a...

Page 6: ...rifuge is to be used for its intended use only Plug the centrifuge only into sockets which have been properly grounded Do not move the centrifuge while it is running Do not lean on the centrifuge Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher Scientific Exceptions to this rule are commercially available glass or plastic centrifuge tubes provided they ...

Page 7: ... could react violently with one another The centrifuge is neither inert nor protected against explosion Never use the centrifuge in an explosion prone environment Do not centrifuge inflammable substances Do not centrifuge toxic or radioactive materials or any pathogenic micro organisms without suitable safety precautions When centrifuging microbiological samples from the Risk Group II according to...

Page 8: ...eneral hazards CAUTION means that material damage could occur WARNING means that injuries or material damage or contamination could occur This symbol refers to biological hazards Observe the information contained in the instruction manual to keep yourself and your environment safe ...

Page 9: ...ion and Description Contents Characteristics of the Heraeus Multifuge X3 F X3 FR on page 1 2 Technical Data on page 1 3 Directives Standards and Guidelines on page 1 4 Functions and Features on page 1 5 Mains Supply on page 1 5 Rotor Selection on page 1 6 ...

Page 10: ...y of speed and RCF or the entry mode These settings can be changed even while the centrifuge is running With the help of the PULSE key you can also centrifuge a sample for just a few seconds if called for The Heraeus Multifuge X3 F X3 FR is equipped with various safety features The housing and rotor chamber consist of steel plate the interior of armour steel while the front panel is made of high i...

Page 11: ...ure 2 Cto 35 C 2 C to 35 C Overvoltage category II II Pollution degree 2 2 Heat dissipation ventilated refrigerated 230 V 120 V 230 V 120 V 6653 BTU h 4776 BTU h 5800 BTU h 4776 BTU h IP 20 20 running time unlimited unlimited max Speed nmax 15200 rpm depending on the rotor 15200 rpm depending on the rotor min Speed nmin 300 rpm 300 rpm maximum RCF value at nmax 25314 x g 25314 x g maximum kinetic ...

Page 12: ... 2 Overvoltage category II IEC 61010 2 101 Emitted interference FCC Part 15 CLASS A NOTE This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses a...

Page 13: ...n brushes Keys and display Easy to clean keypad and display surface Controls Microprocessor controlled Internal memory The most recent data is saved Functions RCF selection temperature control pretemp with cooling equipped devices Acceleration braking profiles 9 acceleration and 10 braking curves Rotor recognition Automatic Imbalance recognition Electronic contingent on rotor and speed Lid lock Au...

Page 14: ...nding rotor operating manuals For more information visit our website at http www thermofisher com TX 750 75003607 with round buckets 75003608 with rectangular buckets 75003614 incl Microtiter carrier 75003617 incl ABI bucket 75003618 BIOshieldTM 1000A 75003603 BIOLinerTM 75003667 HIGHPlateTM 6000 75003606 FIBERLiteTM H3LV 75003665 FIBERLiteTM F14S 6 x 250 LE 75003662 FIBERLiteTM F15 6 x 100 750036...

Page 15: ...ultifuge X3 F X3 FR 2 1 2 Before use Contents Before Setting up on page 2 2 Transporting the Centrifuge on page 2 4 Location on page 2 4 Aligning the Centrifuge on page 2 4 Mains Connection on page 2 5 Storage on page 2 6 ...

Page 16: ...tific immediately if any damage is discovered 2 Remove the packaging 3 Check the order for completeness see Scope of Supply on page iii If the order is incomplete please contact Thermo Fisher Scientific Setting up 1 Place the pallet with the centrifuge so that you have at leased 1 5 m room in front of the pallet 2 Release the rails from the pallet 3 Screw the rails onto the pallet in front of the ...

Page 17: ...ic Multifuge X3 F X3 FR 2 3 4 Remove the protectors that locate the centrifuge on the pallet Release the castors Use several people in order to roll the centrifuge off the pallet 5 Fix the castors once the centrifuge is in its final position ...

Page 18: ...nd free of resonance for example a level laboratory bench The supporting structure must be suitable for horizontal setup of the centrifuge The centrifuge should not be exposed to heat and strong sunlight The set up location must be well ventilated at all times Aligning the Centrifuge The horizontal alignment of the centrifuge must be checked every time after moving it to a different location The s...

Page 19: ...tch on the back press 0 2 Plug the centrifuge into grounded electrical sockets only 3 Check whether the cable complies with the safety standards of your country 4 Make sure that the voltage and frequency correspond to the figures on the rating plate 5 Establish the connection to the power supply with the connecting cable Main Power Switch Mains plug ...

Page 20: ...ific Storage Before storing the centrifuge and the accesories it must be cleanedand if necessary desinfected and decontaminated Store the centrifuge in a clean dust free location Be sure to place the centrifuge on its feet Avoid direct sunlight Fix the castors ...

Page 21: ...Thermo Scientific Multifuge X3 F X3 FR 3 1 3 Control Panel Contents Control Panel on page 3 2 Keys on page 3 2 ...

Page 22: ...le temperature Key Display contents Start Normal start of the centrifuge Stop End run manually Open lid Automatic release possible only when device is switched on Emergency release see Mechanical Emergency Door Release on page 7 2 Pulse By pressing the key PULSE the centrifuge starts immediately and accelerates up to the end speed Releasing the key initiates a stopping process at the highest braki...

Page 23: ...n page 4 2 Lid Opening on page 4 2 Rotor Installation on page 4 2 Entering Parameters on page 4 4 Centrifugation on page 4 10 Temperature Adaptation during Standstill on page 4 11 Short term Centrifugation on page 4 11 Removing the Rotor on page 4 12 Aligning the Centrifuge on page 4 13 ...

Page 24: ...shows The top line shows the message Lid open The bottom line shows the values for the last run Lid Opening 1 Press the OPEN key Close Lid 2 Close the lid by pressing down on it lightly in the middle or on both sides of it Two locks close the lid completely Rotor Installation The approved rotors for the Heraeus Multifuge X3 F X3 FR are listed in section Rotor Selection on page 1 6 Use only the rot...

Page 25: ...tor is properly installed by lifting it slightly on the handle If the rotor can be pulled up then it must be reclamped to the centrifuge spindle CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge CAUTION Do not force the rotor onto the centrifuge spindle If the rotor is very light then it may be necessary to press it onto the centrifuge spindle with a...

Page 26: ...he desired profile by entering a digit between 1 9 on the numeric pad Curve number 1 is the slowest and curve number 9 the fastest 3 Confirm your entry by pressing ENTER Braking curve 1 Press the ACC DEC key twice in order to open the braking curve selection menu The display shows the message Set deceleration 2 Select the desired curve by entering a digit between 1 9 on the numeric pad Curve numbe...

Page 27: ...ll be corrected automatically if the resulting speed is less than 300 rpm This is because 300 rpm is the lowest selectable speed Explanation of RCF Value The relative centrifugal force is given as a multiple of the force of gravity g It is a unitless numerical value which is used to compare the separation or sedimentation capacity of various devices since it is independent of the type of device On...

Page 28: ...e running until you stop it manually with the STOP key Run time mode Use the TOGGLE key to set the run time counter start AT START When AT START is lit the running time will be counted right from pressing the START key AT SPEED When AT SPEED is lit the running time will be counted once the preset speed or the preset RCF value respectively have been reached In this case the selected acceleration cu...

Page 29: ...the desired temperature using the numeric pad The digits show in sequential order 3 Confirm your entry by pressing ENTER Your entry will be automatically confirmed if you do not press any key for 5 seconds Prewarming or precooling the centrifuge For setting the pretemp value for the centrifuge proceed as follows 1 Hold down the TEMPERATURE key for a minimum of three seconds to open the temperature...

Page 30: ...our digits of the bucket catalog number 1 Press the BUCKET key The display shows the following message 2 Press the BUCKET key repeatedly until the bucket being used is displayed Your entry will be automatically confirmed if you do not press any key for 5 seconds Bucket radius Having entered the bucket code you may want to specify the radius When you use adaptors you will have to specify the radius...

Page 31: ...ing a program Programs can be stored and retrieved later by means of the assigned number 1 Enter the program parameters as described above 2 Press the program memory key to load any of the programs 1 5 Press the DIRECTORY key in order to load any of the programs 1 99 by means of the numeric pad Enter the program number and confirm with ENTER key 3 You may want to enter a passcode to protect the da...

Page 32: ...wo hours ago this message will apear in the display Check the castor breaks before pressing the START key again Starting centrifuge program Press the START key on the control panel The centrifuge accelerates to the pre set speed with the time display active If the speed setting is higher than the maximum permissible speed or RCF value for the particular rotor then the display will show the message...

Page 33: ...ted at the designated rate The message END will illuminate and after pressing the key OPEN the lid will open and you can remove the centrifuged material Temperature Adaptation during Standstill The temperature cannot be adapted until the rotor has been positively identified For this the centrifuge must have accelerated to over 500 rpm The speed display will then show END When the rotor is not reco...

Page 34: ...trifuge spindle Make sure not to tilt the rotor while doing this Aerosol tight Rotors When using an aerosol tight lid the rotor can only be removed with the lid closed This is to protect you and the samples Figure 4 2 AutoLockTM lid for aerosol tight rotors CAUTION Rotors supplied with a lid for aerosol tight applications come with a mandrill which belongs to the AutoLockTM Be sure not to place th...

Page 35: ...4 Operation Aligning the Centrifuge Thermo Scientific Multifuge X3 F X3 FR 4 13 Aligning the Centrifuge 1 To turn off the centrifuge put the mains switch to 0 ...

Page 36: ...7 Deutsch 1 English 2 Deutsch 3 Française 4 Español 5 Italiano 6 Nederlands 7 Росский STOP ENTER LCD powersave START 0 no 1 yes ENTER ENTER No Program passcodes 0 0547V011 1 Software and NVRAM identification example display 1 4641V05 ENTER STOP ENTER Set lid passcode ENTER No Auto lid open ENTER ENTER 0547V01 Software ID 2368 Cycle count ENTER ...

Page 37: ...he run Otherwise press 0 no Press ENTER key to confirm Keypad beep Press 1 yes on the numeric pad if the centrifuge should make beep when pressing any key Otherwise press 0 no Press ENTER key to confirm LCD powersave Press 1 yes on the numeric pad if the centrifuge should enter a powdersave mode after the run Otherwise press 0 no Press ENTER key to confirm Lid passcode Press 1 yes on the numeric p...

Page 38: ...ress 1 yes on the numeric pad if the centrifuge should open after the run Otherwise press 0 no Press ENTER key to confirm Software ID Here you fine the current software version Press ENTER key to confirm Cycle count Here you fine the current numbers of cycles Press ENTER key to confirm ...

Page 39: ...ge X3 F X3 FR 6 1 6 Maintenance and Care Contents Cleaning Intervalls on page 6 2 Cleaning on page 6 2 Disinfection on page 6 3 Decontamination on page 6 4 Autoclaving on page 6 5 Service of Thermo Fisher Scientific on page 6 5 ...

Page 40: ...st never exceed 50 C since higher temperatures could damage the material and shorten the lifetime of the parts Use only disinfectants with a pH of 6 8 Dry aluminum parts off with a soft cloth After cleaning treat the entire surface of aluminum parts with corrosion protection oil 70009824 Also treat the cavities with oil Store the aluminum parts at room temperature or in a cold storage room with th...

Page 41: ...uring centrifugation The rotor chamber and the rotor should be treated preferably with a neutral disinfectant CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment CAUTION When cleaning do not allow liquids especially organic solvents to get on the dri...

Page 42: ...abinet at a maximum temperature of 50 C 11 After cleaning treat the entire surface of aluminum parts with corrosion protection oil 70009824 Also treat the cavities with oil 12 Grease the bolt of the swing out rotor with bolt grease 75003786 Decontamination Decantaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation For general radioactive decontaminatio...

Page 43: ...minum parts with corrosion protection oil 70009824 Also treat the cavities with oil 12 Grease the bolt of the swing out rotor with bolt grease 75003786 Autoclaving 1 Before autoclaving clean rotor and accessories and described above 2 Place the rotor on a flat surface Rotors and adapter can beautoclaved at 121 C The maximum permissible autoclave cycle is 20 minutes at 121 C Service of Thermo Fishe...

Page 44: ...entific Thermo Fisher Scientific offers inspection and service contracts for this work Any necessary repairs are performed for free during the warranty period and afterwards for a charge This is only valid if the centrifuge has only been mainained by a Thermo Fisher Scientific service technician ...

Page 45: ...Thermo Scientific Multifuge X3 F X3 FR 7 1 7 Trouble Shooting Contents Mechanical Emergency Door Release on page 7 2 Troubleshooting by User on page 7 3 When to contact Customer Service on page 7 5 ...

Page 46: ...aking process lasts much longer than usual Proceed as follows 1 Make sure the rotor has stopped view port in the lid 2 Pull out the power supply plug 3 On the back of the housing are two black plastic plugs which you can pry out of the back plate with a screwdriver or a knife Pull the release cord attached to it at the same time to trigger the mechanical lid release The lid will open and the sampl...

Page 47: ...braked Bucket code is undefined for the rotor detected check the set points for the given bucket code Is it permitted to use the current bucket in the rotor currently mounted Restart the centrifuge If an error message appears again inform Customer Service Unapproved rotor The centrifuge cannot be operated The run does not start or the centrifuge runs down without being braked Rotor code is not in ...

Page 48: ...ifuge If an error message appears again inform Customer Service E 13 The centrifuge cannot be operated The run does not start or the centrifuge runs down without being braked The check sum in the data memory is incorrect The software corrects errors automatically Check the values of the set point settings etc Restart the centrifuge If an error message appears again inform Customer Service E 15 E 1...

Page 49: ... centrifuge If an error message appears again inform Customer Service E 33 The centrifuge cannot be operated The run does not start or the centrifuge runs down without being braked Overpressure in the refrigeration unit Clean the air inlet for the condenser Restart the centrifuge If an error message appears again inform Customer Service E 34 E 36 The centrifuge cannot be operated The run does not ...

Page 50: ... to contact Customer Service 7 6 Multifuge X3 F X3 FR Thermo Scientific 3 Press and hold the ENTER key until the following message is displayed 4 Communicate the software version to the service technician Software ID XXXXXXXX ...

Page 51: ...yl Alcohol U S S S S M S S S M S S Aluminum Chloride U U S S S S U S S S S M S S S S S S S S S M U U S S Formic Acid 100 S M U U U S M U U S S U S U S U Ammonium Acetate S S U S S S S S S S S S S U S S S S S S S S S S Ammonium Carbonate M S U S S S S S S S S S S S U U S S S S S S M S S S Ammonium Hydroxide 10 U U S U S S M S S S S S S U M S S S S S S S S S M S Ammonium Hydroxide 28 U U S U S U M S...

Page 52: ...ethyl Ether S S U U S S S U S U U S U U U U U U U U U S S S S M U Diethyl Ketone S U U M S U S M U U U M M U S S U U Diethylpyrocarbonate S S U S S S S S U S U S U S S S M S S S S S S Dimethylsulfoxide S S U U S S S S U S S U S U U S S U U S S S S U U Dioxane M S U U S S M M S U U S U M U U M M M U S S S S U U Ferric Chloride U U S M S M S S S S M U S S Acetic Acid Glacial S S U U S S U M S U S U ...

Page 53: ... M S S S Potassium Carbonate M U S S S S S S S S S S S U S S S S S S S S S S S S Potassium Chloride U S S S S S S S S S S S S S S S S S S S S U S S S Potassium Hydroxide 5 U U S S S S M S S S S S U S S S S S S S M U M S U Potassium Hydroxide conc U U M U M M S S U M U U U S M M U U U U Potassium Permanganate S S S S S S S S S U S S S M S M S U S S M S U S Calcium Chloride M U S S S S S S S S S S S...

Page 54: ...5 U U M S S M U S S M S S S M S S S S M S S S M U S M S Sodium Iodide M S S S S S S S S S S S S S S S S S S M S S S Sodium Nitrate S S S S S S S S S S S S S S S S S S S S U S S S S Sodium Sulfate U S S S S S S S S S S S S S S S S S S S S S M S S S Sodium Sulfide S S S S S S S U U S S S M S Sodium Sulfite S S S S S S S M S S S S S S M S S S S S S S S S S Nickel Salts U S S S S S S S S S S S S S S S...

Page 55: ...Acid 50 U U U U S U U S M S U U M U U S S S S M S M U U M M Sulfuric Acid 10 M U U S S U U S S M U S S S S S S S S S S U U U S S Sulfuric Acid 50 M U U U S U U S S M U U S U U M S S S S S U U U M S Sulfuric Acid conc M U U U U U M M U U S U U U M S U M S U U U S Stearic Acid S S S M S S S S S S S S S S S S S M M S S S Tetrahydrofuran S S U U S U U M S U U S U U U M U U U U S U S S U U Toluene S S ...

Page 56: ...S S Citric Acid 10 M S S M S S M S S S S S S S S S M S S S S S S S S S S 1Polyethyleneterephthalate CHEMICAL MATERIAL ALUMINUM ANODIC COATING for ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE Carbon Fiber Epoxy DELRIN ETHYLENE PROPYLENE GLASS NEOPRENE NORYL NYLON PET 1 POLYCLEAR CLEARCRIMP CCCLEARCRIMP POLYALLOMER POLYCARBONATE POLYESTER GLASS THERMOSET POLYTHERMIDE...

Page 57: ...tion of RCF Value 4 5 F Failure message 7 3 Features 1 5 I Imbalance indicator 4 10 Intended Use v K Keys 3 2 Kundendienst 6 5 7 5 L Lid Opening 4 2 Location 2 4 M Mains Connection 2 5 Mains Supply 1 5 2 6 Maintenance 6 1 O Operation 4 1 Overwriting a passcode protected program 4 9 Overwriting a program 4 9 P Passcode 4 9 Precautions iv Preface iii Preselecting RCF 4 4 Preselecting Speed 4 4 Prese...

Page 58: ...4 Select Program 4 9 Short term Centrifugation 4 11 Starting centrifuge program 4 10 Stopping the centrifugation program 4 11 Storage 2 6 Switch on Centrifuge 4 2 T Technical Data 1 3 Temperature Adaptation 4 11 Transporting the Centrifuge 2 4 Trouble Shooting 7 1 ...

Page 59: ... products are available in all countries Please consult your local sales represen tative for details Shown pictures within the manual are examples and may differ considering the set parameters and language Pictures of the user interface within the manual are showing the English version as example Italy 39 02 95059 552 Japan 81 3 5826 1616 Korea 82 2 2023 0600 Netherlands 31 76 579 55 55 New Zealan...

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