Thermo Scientific Heracell Vios 160i CR, Operating Instructions Manual

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3
Description of the Device
Workspace Atmosphere
3-6
Heracell Vios 160i / 250i CR
Thermo Scientific
Under normal operating conditions and at the usual incubation temperature of 37 °C, a constant
relative humidity of approx 93% is achieved in the workspace.

If condensation occurs on the culture containers due to high relative humidity, the humidity in the
workspace can be set to a lower level. The relative humidity in the workspace is lowered from approx.
93% to approx. 90%. The modification requires an extended adaption phase. To ensure that it
effectively prevents dew formation on culture containers, it must be used as a permanent setting.
Instructions for activating the Low Humidity function are given in section “Low Humidity Setting” on
page 6-33 .
Prefilter
Placed on the front section of the water reservoir cover is a prefilter. The prefilter consists of a
dual-layer wire mesh with a silicone frame and is autoclavable as well as heat-resistant. The prefilter
must remain installed in the device during the Steri-Run sterilization routine, but needs to be removed
for refilling the water reservoir.
HEPA Filter and Air Duct
There are 2 HEPA filters in the
Heracell Vios 160i / 250i CR
devices, one in the inner chamber and one in
the rear wall filter enclosure which must be installed by the customer before starting up the device.
CAUTION No tap or ultrapure water in humidity reservoir

Sterile distilled water or water treated to an equivalent quality level is the recommendation for use
in the integrated humidity reservoir. The acceptable conductivity should be within the range of 1 to
20 μS/cm (resistivity should be within the range of 50 K-Ohm-cm to

1 M-Ohm-cm). The pH should be in the range of 7-9. Ultrapure Type 1 water or deionized (DI)
water with a resistivity approaching or equal to 18.2 M-Ohm-cm contains very few ions and will
actively pull ions from interior components, damaging stainless steel, copper, and glass. If there is
only access to DI or Type 1 water, one option is to add a sterile solution of weak sodium
bicarbonate to raise the pH and add ions (recommend 84 mg/l (1 mmol/l).
CAUTION
No chloride containing disinfectants

While stainless steel is resistant to corrosion, it is not corrosion proof. Many chemicals have a
negative effect on stainless steel, especially chlorine and derivatives with oxidizing activity.

The addition of chloride-containing disinfectants or copper sulfate into the water as a constant
disinfectant is not recommended because these can damage the connecting stainless steel/copper
joint of the drain.For cleaning the interior, the recommendation is mild soap solution in water then
rinse to remove residues. Wipe the interior surfaces and parts with a diluted quaternary ammonium
disinfectant. Follow this by wiping with 70% alcohol to remove any remaining traces of the
disinfectant.