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EVOS
™
M5000 Imaging System User Guide
Calibrate the objectives
Calibrate objective
magnification
allows the calibration of the field of view,
parfocality, and parcentration parameters of the selected objective. When
calibrated, parfocality ensures that the sample stays in focus when the objective is
changed, and parcentration ensures that an object in the center of the field of view
stay in the center of the field no matter which objective is being used.
Note:
The pre-installed objectives supplied with the EVOS
™
M5000 Imaging
System have been pre-calibrated. You do not need to calibrate them again unless
they are reinstalled after removal from the instrument.
Calibrate objective
magnification
Calibration procedure requires the use of the EVOS
™
M5000 calibration slide
supplied with the EVOS
™
M5000 Imaging System (Cat. No. AMEP4720). Total
time required objective calibration is about 5 minutes.
Note:
For best parfocality and parcentration, calibrate the installed objectives one
after the other without removing the calibration slide.
1.
Mount the EVOS
™
Calibration Slide
face down
in the vessel holder for slides
(you can use either of the two slots).
2.
Select the objective to calibrate using the Objective selection wheel (page 7).
3.
Go to the
Settings
Objective Selection and Calibration
,
click the
Action
button
for the newly installed objective, then select
Calibrate
to launch the
Objective Calibration tool.
