3
Tuning
Tuning the Reagent Ion Optics
Thermo Scientific
MS/ETD System Getting Started Guide
15
Automatic Tuning of the Reagent Ion Optics
To automatically tune the reagent ion optics
1. Open the Tune Plus window; see the instructions on
2. Click the
On/Off/Standby
button to select the
On
mode.
When clicked, this button cycles through the power modes shown in the left margin.
3. Click the
Display Graph View
(
) button.
On
Off
Standby
Note
When on, the System LED on the front panel of the MS detector turns green and the
high voltage to the electron multipliers turns on.
The following conditions can cause the MS/ETD System to remain in standby mode
even though you try to turn it on:
• Attempting to turn on the reagent ion source when the restrictor heater, source
heater, and the transfer line heater are not at their target temperatures.
• When either the LTQ XL/ETD or LTQ Velos/ETD MS detector or the
MS/ETD System goes into standby mode. Reagent vial nitrogen cooling turns on
if the vials are at an elevated temperature.
• Exception: If you put the LTQ XL/ETD or LTQ Velos/ETD system in standby
mode, the cooling nitrogen turns on after a one hour delay.
• Whenever the pressure in the LTQ XL or LTQ Velos MS detector or the
MS/ETD System exceeds its protection limit. Reagent vial nitrogen cooling turns
on if the vials are at an elevated temperature.
• Whenever the flow of reagent ions becomes insufficient as determined by the
automatic gain control (AGC) setting. When this occurs the LTQ XL/ETD or
LTQ Velos/ETD system completes the Xcalibur sequence step into progress
before going in standby mode. This prevents the loss of analysis results that might
be affected by the reduced reagent ion flow.
