1
Introduction
Tuning and Calibration
Thermo Scientific
MS/ETD System Getting Started Guide
5
Tuning and Calibration
Tune parameters are instrument parameters (for example, ETD optics) whose values can vary
with the type of experiment or analyte being mass analyzed. Tuning the instrument on an
analyte ensures sensitivity and mass resolution.
Calibration parameters are instrument parameters whose values do not vary with the type of
experiment. You calibrate the mass spectrometer to ensure mass accuracy.
To tune and calibrate the LTQ XL/ETD or LTQ Velos/ETD system, perform these basic
steps:
• Tune and calibrate the LTQ XL or LTQ Velos MS detector as described in the
LTQ Series
Getting Started Guide
.
• Tune the optics in the ETD module as described in
Diagnostics
To learn more about some of the diagnostic tools and tests, refer to the
LTQ Series Getting
Started Guide
and the
LTQ Series Hardware Manual
. (At this time, not all diagnostic tools or
tests are discussed in the product guides.)
IMPORTANT
Thermo Fisher Scientific recommends that you calibrate the mass
spectrometer at least once every three months and that you check the calibration about
once a week.
