Thermo Scientific Dionex UltiMate 3000 Series, Operating Instructions Manual

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UltiMate 3000 Series:
NCS-3500RS and NCP-3200RS
Page 72 Operating Instructions
4.5.3 Purging the Pumps
Purging means rinsing the system for a certain time (for the loading pump at a higher flow
rate). Purging is required to
• Remove the 2-propanol with which the module is filled during shipment completely from
the pump before you begin sample analysis.
• Remove air bubbles from the system, for example, after refreshing the solvents in the
reservoirs.
• Remove any previous solvent from the pump when you want to change solvents.
If the pump has not been purged sufficiently, pressure pulsation, a high noise level or
pulsation during the operation of the pump may occur or the analysis may not be
reproducible.
Due to the large liquid volumes in the NC pump and the low flow rates during operation, it is
especially important that the pump is purged sufficiently long. Only then, you can be sure that
the previous solvent has been completely removed when you change solvents. If the previous
solvent has not been removed completely, shifts in retention time may be observed for a
longer period without a reason being obvious.
For information about how purge the
• NC pump, see section 4.5.3.1 ( → page 73).
• Loading pump, see section 4.5.3.2 ( → page 76).