Thermo Scientific Dionex AutoTrace 280, Operator'S Manual

Page: 59 / 166

3 • Operation and Maintenance
Doc. 065330-05 03/2020
51
3.5 Preparing the Dionex AutoTrace 280
1. Turn on the system, using the power switch located on the right side of the
Dionex AutoTrace 280. After power-up, the system goes through a brief
initialization.
NOTE Thermo Fisher Scientific recommends that you leave the
system power on continuously unless the Dionex
AutoTrace 280 will be idle for more than 14 days.
2. To perform a Dry with Gas or Concentrate Sample step:
a. Check that the gas supply has sufficient reserve for the sample run.
b. Turn on the gas supply.
c. Set the pressure to no more than 0.69 MPa (100 psi).
3. Using the regulator knob on the left side of the Dionex AutoTrace 280 (see
Figure 2-4 ), adjust the system's output gas pressure to the proper setting for
the method. The recommended operating setting is 0.07 MPa (10 psi).
4. Check that the solvent reservoirs are full.
5. Check that the tubing is fully submerged in the solvent reservoirs and that
tubing weights and barbed fittings are installed (see Section B.3.1 ).
6. Check that all waste containers (left-side port, elution station solvent, and
elution station aqueous waste) are empty.
7. Select the Prime Solvents diagnostic method:
a. Press
LOAD
to display the
LOADING METHOD
screen.
b. Press LOAD to increment the displayed method to 29.
c. Press
CONT
once to select method 29.
8. Press CONT to run the method. The method draws enough solvent from each
port to prime the liquid lines.
9. Place a collection container in each elution rack position.
10. Install the elution rack onto the two pins on the front of the elution station.
The elution rack is symmetrical and can be positioned either way.
11. (Optional) When using the Dionex AutoTrace 280 cartridge model, manually
rinse the cartridges with MeCl or water. This prevents leakage around the