Thermo Scientific Dionex AutoTrace 280, Operator'S Manual

Page: 111 / 166

5 • Service
Doc. 065330-05 03/2020
103
5.10 Calibrating a Sample Pump
Do not calibrate a sample pump unless the volume removed from the sample
container does not match the sample volume requested in the Load Sample step
of your method.
Parts and Tools Needed
•
Allen wrench, 3/32-in
•
Sample container with approximately 120 mL of water/pump being calibrated
•
Balance
•
1.6-mm (1/16-in) ID tubing (P/N 070358) provided in the Ship Kit (standard
Dionex AutoTrace 280, P/N 071383; Dionex AutoTrace 280 PFAS,
P/N 22136-62005)
Calibrate the Pump
Perform the following procedure on each sample pump that requires calibration.
1. Run a single-step Clean Sample Path method (see Section 3.2.2 ), using
deionized water.
2. Select the Calibrate Pump diagnostic method:
a. Press LOAD to display the LOADING METHOD screen.
b. Press
LOAD
to increment the displayed method to 31.
c. Press CONT once to select method 31.
3. Press
CONT
to load the method.
4. Follow the on-screen instructions as they appear.
5. As instructed, weigh the collection vessel before and after pumping.
Determine the percentage difference between the expected volume (100 mL)
and the delivered volume.
For example, if the method pumps 103 mL instead of 100 mL, the volume is
off by 3%.
6. Locate the pump’s calibration screw in the hole on the right side of the pump
(see Figure 5-12 ).