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3 • Operation and Maintenance

Doc. 065259-05 10/12

31

b. If required for your application, modify the

General

Parameters

. For details about the

General Parameters

, see

.

c. Select the

Edit Mode

. Refer to the following table to determine

the mode to select.

NOTE The injection valve is either the optional Dionex

AS-DV  high-pressure  valve  configured  as  an
injection  valve,  or  the  injection  valve  in  the
chromatography system  module.  The auxiliary
valve  is  the  optional  Dionex  AS-DV  valve  con-
figured as an auxiliary valve.

d. For

Basic Edit Mode

: Select the

Volume

of sample to deliver

from the vial, select a

Bleed

option, and enable or disable a

Rinse

. For details about

Basic Edit Mode

, see

e. For

Advanced Edit Mode

: Add, delete, or modify commands

and parameters in the

Command/Parameters

table. For details

about

Advanced Edit Mode

, see

f. (Optional) If you selected

From Sequence

for the sample

delivery volume, you can use the

Total Volume Delivered

Use Basic mode if you want to …

Use Advanced mode if you want to …

Deliver sample from the vial
specified in the sequence.

Deliver sample from a specified vial and
override the sequence.

Deliver one of the following sample
volumes: volume specified in
sequence, full vial, one-half of a 5 mL
vial, one-third of a 5 mL vial.

Deliver a different volume than is
available in

Basic

mode. The volume can

be specified in 100

P

L increments.

Deliver rinse from the rinse vial range
specified in the Chromeleon 7
Instrument Configuration Manager or
Chromeleon 6.8 Server Configuration
program.

Deliver rinse from any vial.

Perform a bleed with the injection
valve or the auxiliary valve.

Use the optional Dionex AS-DV valve
for a function other than injection or
bleed.

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Summary of Contents for Dionex AS-DV

Page 1: ...Dionex AS DV Autosampler Operator s Manual Document No 065259 Revision 05 October 2012 ...

Page 2: ...t protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous informatio...

Page 3: ...equirements for IC 5 1 4 1 Declaration of Conformity 6 2 Description 7 2 1 Operating Features 7 2 1 1 Front View 7 2 1 2 Front Interior Features 11 2 1 3 Rear Panel 12 2 2 Dionex AS DV Operating Principles 14 2 2 1 Sampling Operating Principles 14 2 2 2 Rinsing Operating Principles 15 2 2 3 Optional High Pressure Valve Operating Principles 15 2 3 Chromeleon Software 18 2 3 1 Software Control Modes...

Page 4: ...omeleon 26 3 4 1 Connecting to Chromeleon 7 26 3 4 2 Connecting to Chromeleon 6 8 28 3 5 Creating a Chromeleon Instrument Method or Program 30 3 5 1 Creating an Instrument or a Program with the Wizard 30 3 5 2 Dionex AS DV General Parameters 32 3 5 3 Basic Edit Mode 33 3 5 4 Advanced Edit Mode 34 3 6 Creating a Chromeleon Sequence 36 3 7 Starting a Run 38 3 8 Operating Details 38 3 8 1 Interruptin...

Page 5: ...nditions 57 4 2 Liquid Leaks 64 4 3 Poor Peak Reproducibility 65 4 4 Unexpected or Extraneous Peaks Detected 66 4 5 Sample Not Loaded 66 4 6 Autosampler Stops Operating 67 4 7 Excessive Backpressure 67 4 8 Autosampler Jammed 68 5 Service 69 5 1 Replacing Tubing and Fittings 69 5 2 Removing the Carousel 70 5 3 Replacing the Sampling Tip and Tubing 74 5 4 Manually Raising the Sampling Head 77 5 5 Re...

Page 6: ...ple Out Line 88 B 3 1 Connecting the Sample Out Line Directly to an IC System 89 B 3 2 Connecting the Sample Out Line to the Optional High Pressure Valve 90 B 4 Connecting the Drain Line 94 B 5 Installing Chromeleon and the Chromeleon Software License 95 B 6 Connecting the Dionex AS DV to the Chromeleon PC 95 B 7 Connecting the Power Cord 96 B 8 Setting Up Chromeleon 97 B 8 1 Installing the Dionex...

Page 7: ...y Control 103 C 1 TTL Input Function 104 C 1 1 Load Cycle Timing 104 C 1 2 Delivery Speed Flush Factor and TTL Homing Delay Settings 104 C 1 3 TTL Input Control Modes 105 C 2 Connecting to the Relay Output and TTL Input 108 C 3 Example Connections for Stand Alone Operation 109 C 4 Controlling the Relay Output 110 D Reordering Information 115 Index ...

Page 8: ...Dionex AS DV Operator s Manual vi Doc 065259 05 10 12 ...

Page 9: ... unique Thermo Scientific Dionex PolyVial sample vial incorporates a cap that acts as a simple piston to force sample out of the vial This eliminates the need for an external sampling pump Sample can be delivered to a sample loop or a concentrator column with backpressures up to 690 kPa 100 psi Caps with a 20 Pm filter are available to remove solid materials from the sample before injection The Di...

Page 10: ...e Help system for Adobe Acrobat or Adobe Reader for assistance Chapter 1 Introduction Introduces the Dionex AS DV explains the conventions used in this manual including safety related information Chapter 2 Description Describes Dionex AS DVoperating features and the software required for autosampler control Chapter 3 Operation and Maintenance Provides operating instructions for the Dionex AS DV an...

Page 11: ...s manual contains warnings and precautionary statements that when properly followed can prevent personal injury to the operator and or damage to the Dionex AS DV Safety messages appear in bold type and are accompanied by icons as shown here Appendix C Relay Control Provides details about setting up the Dionex AS DVfor relay control operation Appendix D Reordering Information Lists part numbers of ...

Page 12: ... évitée pourrait entraîner des blessures graves à mortelles Signale une situation de danger potentiel qui si elle n est pas évitée pourrait entraîner des blessures mineures à modérées Également utilisé pour signaler une situation ou une pratique qui pourrait gravement endommager l instrument mais qui n entraînera pas de blessures Bedeutet unmittelbare Gefahr Mißachtung kann zum Tod oder schwerwieg...

Page 13: ...ent Primary protective conductor terminal Secondary protective conductor terminal Power supply is on Power supply is off Indicates a potential hazard Refer to this operator s manual for an explanation of the hazard and how to proceed Contaminant Specification Ions Resistivity 18 0 megohm cm Organics TOC 10 ppb Iron Transition Metals 1 ppb Pyrogens 0 03 Eu mL Particulates 0 2 µm 1 units mL Colloids...

Page 14: ...DV Operator s Manual 6 Doc 065259 05 10 12 1 4 1 Declaration of Conformity The cETLus and CE marks on the Dionex AS DV model data label indicate that the autosampler is in compliance with the following standards ...

Page 15: ... Features 2 1 1 Front View Figure 2 1 illustrates the main operating features of the Dionex AS DV Figure 2 1 Dionex AS DV Front View Sampling Head Tower Vial Carousel LED Display Front Cover Carousel Cover Carousel Release Button Not visible in photo ...

Page 16: ...e sampling mechanism operates see Section 2 2 1 The carousel holds the PolyVials containing sample or rinse solution Vial numbers are molded into the top of the carousel to indicate the sampling position Vials can be placed in any order in the carousel The Chromeleon sequence controls the order in which sampling occurs For a description of how to create a sequence see Section 3 6 Sensors in the sa...

Page 17: ...DV Ship Kit for 0 5 mL vials P N AAA 071115 PolyVials serve both rinse and sample functions NOTE Thermo Fisher Scientific does not recommend the use of flammable solvents in the Dionex AS DV vials Vial Caps Vial caps perform an important function in sample loading see Section 2 2 1 They also minimize evaporation contamination or spills of sample while awaiting use Caps with 20 Pm filters are avail...

Page 18: ...al see Figure 2 3 After you load the vial into the carousel twist the adapter slightly to the right to lock the adapter in place Vial Tray Vial trays either for 0 5 mL or 5 0 mL vials are included in the respective Dionex AS DV Ship Kit P N AAA 071115 Ship Kit for 0 5 mL vials P N 068887 Ship Kit for 5 0 mL vials Place vials into the tray and then fill them until the liquid in the vials reaches th...

Page 19: ... Dionex AS DV To access the front interior remove the front cover by gripping the indentations at each side and pulling the cover straight off toward you Figure 2 4 Vial Tray 0 5 mL Version Shown Figure 2 5 Front Interior View with Optional High Pressure Valve Fill vials to this level Optional Valve Sample Out line ...

Page 20: ...d or to the injection valve in the IC system High Pressure Valve Optional Either a 6 port or a 10 port high pressure valve can be installed in the Dionex AS DV The valve can be configured to operate as an injection valve or an auxiliary valve in an application for example it can be used as a bleed valve or to load a concentrator column For details about operation of the high pressure valve see Sec...

Page 21: ...e drain hose is included in the Dionex AS DV Ship Kit Figure 2 6 Dionex AS DV Rear Panel Neutralize acidic and caustic wastes before disposal Dispose of all wastes in accordance with local regulations Neutralisez les déchets acides ou caustiques avant de les jeter Jetez les déchets aux règlements locaux Neutralisieren Sie säurehaltige und ätzende Abfälle vor ihrer Entsorgung Entsorgen Sie alle Abf...

Page 22: ...ndix C Power Switch Fuse Drawer and Power Receptacle The power switch provides on off control of power to the Dionex AS DV The fuse drawer contains two fast blow IEC 127 fuses rated 3 15 A P N 954745 For instructions on how to change the fuses see Section 5 7 The power supply cord plugs into the IEC 320 three prong receptacle The power supply cord is used as the main disconnect device Make sure th...

Page 23: ... pump The concave bottom of the vial cap traps air so that it can be expelled with any excess sample above the fill line In addition the shape exactly matches the convex bottom of the sample vial when the cap is pushed completely into the vial virtually all of the sample is expelled The socket for the sampling tip is identical on both sizes of caps to allow mating with the tip of the sampler head ...

Page 24: ... reserved for rinse vial use only The rinse vial range is defined in the Chromeleon 7 Instrument Configuration Manager or the Chromeleon 6 8 Server Configuration program see Section B 8 2 When performing a rinse the Dionex AS DV takes the rinse liquid from the first vial in the range If the first vial is empty the next vial in the range is used and so on To define rinse cycles in Chromeleon see Se...

Page 25: ...c Dionex Application Note for more information Operation as a Bleed Valve When the optional valve is configured as a bleed valve see Figure 2 10 and Figure 2 11 the valve directs excess sample and any air trapped in the sample vial to waste Excess sample is any sample above the fill line on the vial see Figure 2 7 After the initial bleed the valve position is switched and the sample is sent direct...

Page 26: ...AS DV Sample Out Line To Waste PolyVial AS DV Optional Valve A Position L 4 1 L 5 S 6 W P 2 C 3 Sample Loop Concentrator Column or To Waste From Pump To Column Injection Valve Load Position IC System AS DV Sample Out Line To Waste L 4 1 L 5 S 6 W P 2 C 3 PolyVial AS DV Sample Loop Concentrator Column or To Waste From Pump To Column Injection Valve Load Position IC System Optional Valve A Position ...

Page 27: ... Modes Two modes of software control are available direct control and automated control With direct control you select operating parameters and commands from the Dionex AS DV ePanel on the Chromeleon 7 ePanel Set see Figure 2 13 or from the Control panel on the Chromeleon 6 8 panel tabset see Figure 2 13 Direct control commands are executed as soon as they are entered Figure 2 12 Chromeleon 7 ePan...

Page 28: ...tomatically with the help of a software wizard For information about creating an instrument method or a program see Section 3 5 2 3 2 System Wellness System Wellness monitors the overall health of a chromatographic system It provides built in diagnostic and calibration features that help prevent unscheduled system shutdowns and assure reliable operation of system devices Use the Dionex AS DV Welln...

Page 29: ...e Error Counter increments each time the needle jams The Speed Adjustment Counter increments each time the speed is adjusted due to excessive liquid backpressure or a clog The Vial Process Counter increments each time the carousel moves a vial into place for processing The Valve Actuation Counter increments each time the position of the high pressure valve if installed is switched Figure 2 14 Dion...

Page 30: ...Dionex AS DV Operator s Manual 22 Doc 065259 05 10 12 ...

Page 31: ...e cleaning inspect each vial and cap for damage nicks scratches etc 3 1 1 Cleaning the Vials 1 Rinse the vials 10 times with a jet of deionized water 2 Place the rinsed vials in a vial tray for 0 5 mL vials P N 068948 for 5 0 mL vials P N 068947 and fill them to the top with deionized water Do not touch the tops or interiors of the vials with your fingers 3 Allow the vials to soak for a minimum of...

Page 32: ...wing procedure to clean caps with filters 1 Place caps into a clean 4 liter polyethylene container Fill the container three quarters full with deionized water and cap 2 Vigorously agitate the container for 30 seconds Drain the water 3 Empty the caps onto a clean surface 4 Using a clean syringe force 50 mL of deionized water through the filter frit from the top of the cap Be sure not to dislodge th...

Page 33: ... 068947 2 Fill the vials with either sample or rinse solution until the liquid in the vials reaches the top of the tray see Figure 3 1 Using a pipette simplifies filling the 0 5 mL vials After filling inspect the vials to make sure no air bubbles are trapped at the bottom NOTE Do not underfill the vials This may result in insufficient sample being loaded for the first injection A cap must be insta...

Page 34: ...987 see Figure 3 3 to press the cap into the vial to the proper depth the top of the cap is flush with the lip of the vial The tool is provided in both Dionex AS DV Ship Kits for 0 5 mL vials P N AAA 071115 for 5 0 mL vials P N 068887 NOTE Do not use the concave end of the cap insertion tool for installing caps on Dionex AS DV vials It is for use with another autosampler Figure 3 2 Caps Partially ...

Page 35: ...e button on the inside of the chassis Pressing the Carousel Release button is required for two purposes To disengage the carousel from the motor To reset vial memory clear presence vial size and volume remaining information Before starting a run always press Carousel Release to reset the vial information 1 If you are using 0 5 mL vials insert a vial adapter onto the vial 2 Load the vials into the ...

Page 36: ...nstrument Controller Service is running idle If the icon is crossed out in red the Instrument Controller Service is not running Right click the icon and click Start Chromeleon Instrument Controller The icon changes to gold to indicate that the Instrument Controller Service is starting If the Chromeleon Tray icon is not on the Windows taskbar click Start All Programs Chromeleon 7 Services Manager t...

Page 37: ...n and Maintenance Doc 065259 05 10 12 27 Pane Chromeleon 7 connects to the instrument and displays the ePanel Set Click the AS DV tab see Figure 3 4 Figure 3 4 Example Chromeleon 7 ePanel Set Dionex AS DV ePanel ...

Page 38: ...stopped To start the server right click the icon and select Start Server The icon changes to gold to indicate that the Chromeleon 6 8 Server is starting If the Server Monitor icon is not on the Windows taskbar click Start and select All Programs Chromeleon Server Monitor The Server Monitor opens Click Start to start the server 3 To start the Chromeleon client click Start and select All Programs Ch...

Page 39: ... Operation and Maintenance Doc 065259 05 10 12 29 5 To display the Dionex AS DV Control panel click the AS DV tab see Figure 3 5 Figure 3 5 Example Chromeleon 6 8 Panel Tabset Dionex AS DV Control Panel ...

Page 40: ...running see Section 3 4 2 Start the Chromeleon 7 Instrument Method Wizard or Chromeleon 6 8 Program Wizard For Chromeleon 7 On the Console on the menu bar click the arrow next to Create and then click Instrument Method Select the instrument in which the Dionex AS DV is configured and click Next For Chromeleon 6 8 Select File New Select Program File and click OK or double click Program File Select ...

Page 41: ...and parameters in the Command Parameters table For details about Advanced Edit Mode see Section 3 5 4 f Optional If you selected From Sequence for the sample delivery volume you can use the Total Volume Delivered Use Basic mode if you want to Use Advanced mode if you want to Deliver sample from the vial specified in the sequence Deliver sample from a specified vial and override the sequence Delive...

Page 42: ... to 500 µL The default value of 125 µL is appropriate for the 1 4 m 56 in length of sample out line provided with the Dionex AS DV You can set the Delay Volume in the Chromeleon 7 Instrument Configuration Manager or the Chromeleon 6 8 Server Configuration program see Section B 8 2 Cycle Time To set a uniform time between injections select this check box and enter the time in minutes The time betwe...

Page 43: ...ex AS DV uses the injection volume and vial position specified in the sequence EndSamplePrep Identifies the end of a group of sample preparation commands This command is used when sample overlap operation is enabled in the Chromeleon 7 Instrument Configuration Manager or Chromeleon 6 8 Server Configuration program see Section B 8 2 Inject Chromeleon 6 8 only Injects the sample You can modify the l...

Page 44: ...ommand Parameters table determines the order in which they will be executed You can add delete and modify commands and select additional commands and parameters not available in Basic Edit Mode Advanced Edit Mode Commands LoadPosition Sets the injection valve to the load position InjectPosition Sets the injection valve to the inject position DeliverRinse Delivers rinse fluid through the sample out...

Page 45: ...in the Command Parameters table first select the command listed immediately below the intended location of the new command Then in the Command list select the command to be added enter the command parameters if any and click Insert The original command remains selected to allow you to easily add commands in chronological order To change an existing command select the command in the table change th...

Page 46: ...ails about a page click the Help button on the page On the Unknown Injections or Unknown Samples page define one or more injections On the Standard Samples page in Chromeleon 6 8 only define one or more standard injections or click Next to skip this page On the Methods Reporting page specify an instrument method or a program that includes commands for Dionex AS DV operation see Section 3 5 Notes W...

Page 47: ...wizard click Finish Chromeleon 7 or Done Chromeleon 6 8 The sequence opens on the Chromeleon 7 Console see Figure 3 6 or in the Chromeleon 6 8 Browser see Figure 3 7 Figure 3 6 Example Sequence on the Chromeleon 7 Console Figure 3 7 Example Sequence in the Chromeleon 6 8 Browser ...

Page 48: ...e injection list Specify when to stop immediately after the current sample or after the current sequence To interrupt the autosampler when a sequence is running in Chromeleon 6 8 Select Batch Stop on the Chromeleon menu bar Select when to stop immediately after the current sample or after the current sequence Before starting a run always press the Carousel Release button to disengage the carousel ...

Page 49: ...on the vial see Figure 2 7 You can use either the injection valve or an optional high pressure valve configured as an auxiliary valve to perform the bleed For details about plumbing a high pressure valve as a bleed valve see Section 2 2 3 Enabling a Bleed 1 If you are creating a new instrument method or program go to the Sample Preparation Options page of the Instrument Method Wizard or Program Wi...

Page 50: ...ual 40 Doc 065259 05 10 12 Figure 3 9 For suggested valve plumbing connections see Plumbing the Optional Valve on page 92 Figure 3 8 Sample Preparation Options Chromeleon 6 8 Version Shown Bleed Enabled Using the Injection Valve ...

Page 51: ...alve position to direct the bleed volume to waste The DeliverSample Volume Bleed command delivers the excess sample and trapped air from the vial to the valve 3 If you are using Advanced Edit Mode manually add the two commands described above to the Command Parameters table Figure 3 9 Sample Preparation Options Chromeleon 6 8 Version Shown Bleed Enabled Using the Auxiliary Valve ...

Page 52: ...strument method or program go to the Sample Preparation Options page of the Instrument Method Wizard or Program Wizard see Figure 3 8 If you are editing an existing instrument method or program go to the Sample Preparation page of the Instrument Method Editor or Program Editor 2 If you are using Basic Edit Mode Select the Rinse check box and enter the Volume see Figure 3 10 With this mode the rins...

Page 53: ...d Maintenance Doc 065259 05 10 12 43 Server Configuration program see Specifying the Rinse Vial Position on page 45 Figure 3 10 Sample Preparation Options Chromeleon 6 8 Version Shown Deliver Rinse in Basic Edit Mode ...

Page 54: ...irecting the rinse volume to waste The DeliverRinse command delivers the specified rinse Volume from the Rinse vial to the valve 3 If you are using Advanced Edit Mode Manually add the InjectPosition and DeliverRinse commands described above to the Command Parameters table see Figure 3 11 With this mode you can deliver the rinse from any vial Figure 3 11 Sample Preparation Options Deliver Rinse in ...

Page 55: ...e Options tab Under Rinse Vial Range define a range of vial positions to be reserved for rinse vial use only see Figure 3 12 2 In the Chromeleon instrument method or program enter Rinse for the vial position See the example in Figure 3 10 When the Dionex AS DV performs the rinse it takes the rinse liquid from the first vial in the rinse vial range If the first vial is empty the next vial in the ra...

Page 56: ...method or program 1 On the Sample Preparation page of the Instrument Method Wizard Editor or Program Wizard Editor select Advanced Edit Mode NOTE When you open the Sample Preparation page in the Instrument Method Editor or Program Editor Advanced Edit Mode is selected by default 2 Enter a DeliverRinse command and enter the preferred vial position Figure 3 13 Sample Preparation Options Chromeleon 6...

Page 57: ...n positions 2 and 3 respectively For Sample 4 the Dionex AS DV returns to the vial in position 1 3 8 5 Replacing Vials During a Run During a run follow the steps below to remove vials that have already been sampled and replace them with different vials 1 Open the Dionex AS DV ePanel in Chromeleon 7 see Figure 3 4 or Control panel in Chromeleon 6 8 see Figure 3 5 2 Under Reset Vial Memory enter the...

Page 58: ...Enter injection information for the new sample and click File Save 4 If you are replacing a previously sampled vial with the priority vial follow these steps a Open the Dionex AS DV ePanel in Chromeleon 7 see Figure 3 4 or Control panel in Chromeleon 6 8 see Figure 3 5 b Under Reset Vial Memory enter the position of the vial in the Start Pos and End Pos boxes c Click Reset Vial Range This clears v...

Page 59: ...ial vial volume in multiple injections Sample delivery volume features vary depending on the Edit Mode Controlling the Delivery Volume in Basic Edit Mode 1 On the Sample Preparation page of the Instrument Method Wizard Editor or Program Wizard Editor select Basic Edit Mode see Figure 3 15 Figure 3 15 Sample Preparation Options Chromeleon 6 8 Version Shown Basic Edit Mode ...

Page 60: ...r or Program Wizard Editor select Advanced Edit Mode 2 If you are creating a new instrument method or program add a DeliverSample command to the list of commands in the Command Parameter table 3 If you are editing an existing instrument method or program click the Deliver Sample command in the list 4 In the Volume list select the preferred option The options available in Advanced Edit Mode are Fro...

Page 61: ... However they are ignored in this example If a second DeliverSample command without a specified volume were included the DelayVolume and FlushFactor commands would be used to calculate the volume for the second DeliverSample command In the Script or Commands view you can enter the Volume for the DeliverSample command in 1 PL increments For example to take three injections from a 5000 PL vial enter...

Page 62: ...verSample command is included without a specified volume In the example shown in Figure 3 17 the loop volume is 25 PL the delay volume is 125 PL the default and the flush factor is 8 This provides a delivery volume of 1200 PL This example allows four injections per filled 5 0 mL vial Notes In Loop Mode you can increase or decrease the delivery volume by entering a different Flush Factor Figure 3 1...

Page 63: ...reparation functions This means that while data collection is occurring for one injection the Dionex AS DV performs the sample preparation steps for the next injection in the sequence The overlapped steps include Dionex AS DV commands that occur before the EndSamplePrep command in the instrument method or program For sample overlap to occur the BeginOverlap command must also be present in the inst...

Page 64: ...s Manual 54 Doc 065259 05 10 12 Program Wizard Chromeleon automatically adds the EndSamplePrep and BeginOverlap commands See the example in Figure 3 18 Figure 3 18 Chromeleon 6 8 Commands View Example Sample Overlap Enabled ...

Page 65: ...the Instrument Configuration Manager or Server Configuration program open the Dionex AS DV Properties dialog box and click the Options tab Select the Sample Overlap check box see Figure 3 19 Figure 3 19 Dionex AS DV Configuration Properties Options Tab Page with Sample Overlap Enabled ...

Page 66: ...ort for Dionex products In the U S and Canada call 1 800 346 6390 Outside the U S and Canada call the nearest Thermo Fisher Scientific office 3 9 1 Periodic Maintenance Periodically check the carousel and wipe up any spills Periodically rinse off any dried spills 3 9 2 Annual Maintenance Rebuild the optional high pressure valve see Section 5 5 Replace the sampling tip and the tubing between the ti...

Page 67: ...tific office 4 1 Alarms and Error Conditions If any of the following alarm conditions occurs an error message is displayed in the Chromeleon audit trail Each error message is preceded by an icon that identifies the seriousness of the underlying problem see the table below Icon Severity Level Description Warning A message is displayed in the audit trail but the current run is not interrupted Error ...

Page 68: ...ver Warning page 60 Carousel or needle is busy Abort page 60 More rinse volume than remaining has been requested Abort page 60 The motor speed is reduced to of the previous one because of excessive load Warning page 60 Needle is jammed Abort page 61 Not enough volume to deliver Error page 61 Sample needle failed to reach destination Error page 61 This command is not allowed at this stage because t...

Page 69: ...e Carousel Release button or clicking a Reset Vial Memory button on the Dionex AS DV Control panel see Figure 3 5 when the needle is down To troubleshoot 1 Wait until the needle is raised homed before resetting vial memory 2 If the problem persists contact Technical Support for Dionex products for assistance This error can occur if the carousel is jammed or the vial sensor has failed To troublesho...

Page 70: ...cified for the rinse To troubleshoot 1 Press the Carousel Release button to clear the error and reset the system 2 Install filled vials in the rinse vial range or in the specified position 3 Begin the sampling process again This error occurs when the needle is jammed or there is excessive backpressure The motor speed is reduced and the Dionex AS DV attempts to continue delivery If the problem pers...

Page 71: ...r Dionex products for assistance This error occurs when there is insufficient liquid in the vial to deliver the requested volume To troubleshoot Add more sample to the vial or select a smaller volume in Chromeleon see Section 3 8 7 This error occurs when the carousel is blocked or the sample head is jammed To troubleshoot 1 Remove the carousel see Section 5 2 and examine for blockages 2 If the pro...

Page 72: ...f the problem persists contact Technical Support for Dionex products for assistance This error occurs if the optional high pressure valve is not connected properly or if the valve fails to switch position within 1 second of being toggled To troubleshoot 1 Check the valve connections a Start the Chromeleon 7 Instrument Configuration Manager or the Chromeleon 6 8 Server Configuration program For Chr...

Page 73: ...u bar b Turn off the Dionex AS DV power briefly and then restart NOTE Turning off the Dionex AS DV power does not reset vial memory c On the Dionex AS DV ePanel or Control panel try to toggle the valve position by clicking first the Load Position or Position A button and then the Inject Position or Position B button 3 If the error recurs rebuild the valve see Section 5 5 This error occurs if the c...

Page 74: ...or instructions see Section 3 8 5 3 If the problem persists contact Technical Support for Dionex products for assistance 4 2 Liquid Leaks Old previously used caps or sample vials Do not reuse caps or sample vials Damaged tubing between sampling tip and optional high pressure valve in the Dionex AS DV or injection valve in the IC system Replace the sampling tip and tubing see Section 5 3 The vial s...

Page 75: ...ly After pushing the cap into the vial gently tap any excess liquid from the fill cap socket Bleed function not turned on when loading a concentrator column Before loading concentrator column check that Bleed is selected in Chromeleon see Section 3 8 2 This ensures that a uniform sample volume is delivered to the concentrator regardless of how accurately the vials are filled Bleed also prevents th...

Page 76: ...vial perform a bleed to direct excess sample and any air trapped in the vial to waste For instructions see Section 3 8 2 The bleed can be performed using either the injection valve or an optional high pressure valve 3 If you are making multiple injections per vial make them sequentially This allows the needle to remain in the vial between injections thus preventing air from entering the sample 4 4...

Page 77: ...ecorded in the Chromeleon audit trail See Section 4 8 for troubleshooting 4 7 Excessive Backpressure Injection valve in wrong position On the Dionex AS DV ePanel in Chromeleon 7 or Control panel on the panel tabset in Chromeleon 6 8 toggle the valve position Restriction in the liquid flow path 1 If the high backpressure persists follow the liquid flow path backward from the injection valve through...

Page 78: ...rousel is jammed 1 Press the Carousel Release button to try to home the carousel 2 Remove the carousel see Section 5 2 and check for foreign objects Sampling tip jams as it is being withdrawn 1 Check for excessive backpressure see Section 4 7 2 Check the sampling tip for possible obstruction a Turn off the power to the Dionex AS DV b Manually raise the sampling head see Section 5 4 c Check for blo...

Page 79: ...The Dionex AS DV is plumbed with the needle and tubing assembly listed below Dionex AS DVtubing connections use high pressure 10 32 fitting bolts P N 074449 and high pressure double cone ferrules P N 074373 To tighten a high pressure fitting bolt and ferrule 1 Use your fingers to tighten the fitting bolt as tight as you can 2 Then use a wrench to tighten the fitting an additional three quarter tur...

Page 80: ...to its fully up position 2 Turn off the Dionex AS DVpower and disconnect the power cord Raise the top cover 3 Remove the Dionex AS DV front cover by gripping the indentations at each side and pulling the cover straight off toward you 4 Use a 1 Phillips screwdriver to remove the three flathead screws from the sampling head tower cover see Figure 5 1 Lift up the cover to remove it Figure 5 1 Removin...

Page 81: ...f slack in the line Then slide the tubing out of the slot in the sampling head see Figure 5 3 7 Push down on the sampling head to move the needle into the carousel a small amount Twist the needle to allow the sampling tip to exit through the opening in the sensor block Figure 5 3 Sliding the Sample Out Line Out of the Sampling Head Slot Sampling Head Remove the set screw Slide the line out of the ...

Page 82: ...igure 5 4 9 Use a 2 5 mm Allen wrench to remove the three screws on the sensor block see Figure 5 5 Lift the sensor block off the carousel and set it to the right Figure 5 5 Removing the Sensor Block Figure 5 4 Lifting the Needle Out of the Carousel Lift the needle Set the needle to the right Remove 3 screws Sensor Block ...

Page 83: ...needle tubing back into the slot in the sampling head 14 Reinstall the sensor block 15 Align the flat sides of the sampling tip so that they fit through the opening in the sensor block 16 Make sure that the needle is pushed as far as it will go up into the sampling head and then tighten the set screw firmly NOTE The set screw must be very tight to secure the sampling needle 17 Reinstall the sampli...

Page 84: ...ing tools are needed to complete this procedure 1 Phillips screwdriver and 3 mm Allen wrench 1 Disconnect the sample out line from the injection valve or optional high pressure valve Remove the fitting 2 Press the Carousel Release button to retract the sampling tip to its fully up position 3 Turn off the Dionex AS DV power and disconnect the power cord Raise the top cover 4 Remove the Dionex AS DV...

Page 85: ... sampling head to move the needle into the carousel a small amount Twist the needle to allow the sampling tip to exit through the opening in the sensor block Figure 5 9 Sliding the Sample Out Line Out of the Sampling Head Slot 9 Unscrew the sampling tip from the needle and pull out and discard the tip and tubing 10 Locate the sampling tip assembly in the kit This assembly consists of a length of t...

Page 86: ...bing back into the slot in the sampling head 14 Align the flat sides of the sampling tip so that they fit through the opening in the sensor block 15 Make sure that the needle is pushed as far as it will go up into the sampling head and then tighten the set screw firmly NOTE The set screw must be very tight to secure the sampling needle 16 Reinstall the sampling head tower cover 17 Reinstall the fr...

Page 87: ...river and 2 5 mm Allen wrench 1 Turn off the Dionex AS DVpower and disconnect the power cord Raise the top cover 2 Remove the Dionex AS DV front cover by gripping the indentations at each side and pulling the cover straight off toward you 3 Use a 1 Phillips screwdriver to remove the three flathead screws from the sampling head tower cover see Figure 5 1 Lift up the cover to remove it Figure 5 10 R...

Page 88: ... the obstruction from the carousel Figure 5 11 Manually Raising the Sampling Head 7 Turn the wrench counterclockwise to begin lowering the needle Turn the wrench until the top of the sampling head is about 6 mm 1 4 in below the top of the sampling mechanism see Figure 5 12 Figure 5 12 Lowering the Sampling Head Before Turning on the Power 8 Reinstall the sampling head tower cover 9 Reinstall the f...

Page 89: ...mance and void the product warranty NOTE If you prefer you can replace the high pressure valve pod see Section 5 6 instead of rebuilding the valve The following tool is needed to complete this procedure 3 mm Allen wrench 1 Turn off the Dionex AS DVpower 2 Raise the top cover 3 Remove the Dionex AS DVfront cover by gripping the indentations at each side and pulling the cover straight off toward you...

Page 90: ...igure 5 13 b Slide the valve assembly partway out of the chassis press the locking latch on the cable see Figure 5 14 and disconnect the cable c Remove the valve assembly 6 Follow the instructions provided in the Rebuild Kit to replace the rotor seal isolation seal and stator face Figure 5 13 Removing the Valve Assembly Figure 5 14 Disconnecting the Valve Cable Remove these 2 screws Locking Latch ...

Page 91: ...sure valve NOTE Substitution of non Dionex Thermo Scientific parts may impair valve performance and void the product warranty The following tool is needed to complete this procedure 3 mm Allen wrench 1 Turn off the Dionex AS DV power 2 Raise the top cover 3 Remove the Dionex AS DV front cover by gripping the indentations at each side and pulling the cover straight off toward you 4 Disconnect each ...

Page 92: ... 5 15 b Slide the valve assembly partway out of the chassis press the locking latch on the cable see Figure 5 16 and disconnect the valve cable c Remove the valve assembly 6 Unscrew the black locking ring on the front of the valve see Figure 5 15 and remove the ring Figure 5 15 Removing the Valve Assembly Figure 5 16 Disconnecting the Valve Cable Remove these 2 screws Black Locking Ring Locking La...

Page 93: ...on the pod align with the matching splines inside the valve holder see Figure 5 17 If necessary twist the end of the pod to adjust the position of the splines 10 Push the pod into the holder until it clicks into place Replace the black locking ring 11 Reinstall the valve assembly and reconnect all liquid lines 12 Turn on the power to the Dionex AS DV 13 Start a test run to check for leaks from the...

Page 94: ...lace the two fuses with new 3 15 A IEC 127 fast blow fuses P N 954745 Thermo Scientific recommends always replacing both fuses Figure 5 18 Dionex AS DV Fuse Drawer 6 Insert the fuse drawer into its compartment Apply sufficient pressure evenly against the drawer to engage the two locks The drawer will be flush against the panel when both locks are engaged 7 Reconnect the main power cord and turn on...

Page 95: ...o 60 Hz 45 W Typical input power 15 W Maximum line draw 0 22 A at 110 Vac Fuses Two fast blow IEC 127 fuses rated at 3 15 A P N 954745 Dimensions Width 44 45 cm 17 5 in Height 23 cm 9 in Depth 56 cm 22 in Weight 16 kg 35 lbs Operating Temperature 10 to 35 C 50 to 95 F Humidity 5 to 95 relative humidity noncondensing ...

Page 96: ...r of Samples 50 Minimum Volume Delivered 0 1 mL Valve Optional 6 port or 10 port 2 position valve with PEEK wetted components electrically activated Volume 0 5 mL and 5 0 mL nominal Filter Optional 20 Pm nominal Material Pharmaceutical grade polypropylene vial and polyethylene cap sintered polyethylene filter Contamination Levels Less than 3 0 ppb fluoride chloride nitrate and phosphate 8 0 ppb su...

Page 97: ...ace required For optimum operation place the Dionex AS DV close as possible to the left side of the IC system Allow at least 38 cm 15 in of free space behind the Dionex AS DV for connections and ventilation Be sure that there is sufficient clearance above the Dionex AS DVto allow the top cover to be opened or removed B 2 Unpacking the Dionex AS DV 1 Open the Dionex AS DV shipping container Remove ...

Page 98: ...ientific recommends that you wear gloves while connecting the Dionex AS DV sample out line 1 Raise the Dionex AS DV top cover 2 Remove the Dionex AS DV front cover by gripping the indentations at each side and pulling the cover straight off toward you The sample out line is threaded through an aperture in the chassis see Figure B 1 Save the shipping container for later use Should it be necessary t...

Page 99: ...rectly to an IC System 1 Route the sample out line through the side slot in the Dionex AS DV chassis Leave a little slack in the tubing 2 Locate a 10 32 fitting bolt P N 074449 and double cone ferrule P N 074373 in the Dionex AS DV Ship Kit for 0 5 mL vials P N AAA 071115 for 5 0 mL vials P N 068887 3 Install the fitting and ferrule onto the end of the Dionex AS DV sample out line See Section 5 1 ...

Page 100: ...n B 4 B 3 2 Connecting the Sample Out Line to the Optional High Pressure Valve Installing the Optional Valve 1 Verify that the power to the Dionex AS DV is off 2 Use a 3 mm Allen wrench to remove the two screws holding the valve cover plate to the chassis Save the screws The Dionex AS DV power must be turned off before you install a valve If the power is on when you connect or disconnect the valve...

Page 101: ... 068921 5 Align the electrical cable with the connector on the back of the valve see Figure B 4 6 Press the cable s locking latch and insert the cable into the connector on the valve until you hear a click 7 Slide the valve assembly into the valve recess making sure not to pinch the cable 8 Use the two screws you removed from the valve cover plate to secure the valve onto the chassis Hand tighten ...

Page 102: ...ve see Figure B 5 Plumbing the 6 port valve as a bleed valve see Figure B 6 Plumbing the 10 port valve as a bleed valve see Figure B 7 For information about plumbing the valve for other functions refer to the appropriate Thermo Scientific Dionex Application Note Figure B 5 6 Port Valve Plumbing for Injection Valve Figure B 6 6 Port Valve Plumbing for Bleed Valve 4 1 5 6 2 3 To Waste AS DV Sample O...

Page 103: ...valve connect the waste line to port 8 d Route the other end of the waste line to a waste container Make sure that the outlet of the waste line is below the level of the Dionex AS DV and is not elevated at any point 2 Connect the Dionex AS DV sample out line a Install a fitting and ferrule onto the end of the Dionex AS DV sample out line b Connect the sample out line to the appropriate port on the...

Page 104: ...IC system Use a fitting and ferrule appropriate for your IC system 4 To complete the 6 port valve plumbing for injection valve operation refer to Figure B 5 and to the manual for the IC system 5 Replace the front cover and close the top cover on the Dionex AS DV Go on to Section B 4 B 4 Connecting the Drain Line 1 Locate the corrugated drain hose P N 055075 in the Dionex AS DV Ship Kit 2 Push the ...

Page 105: ...n the host IC system A USB 2 0 external hub can be used to connect the Dionex AS DV to the PC Refer to the manual provided by the hub vendor for complete installation instructions One or more hubs is required in the following situations Use a hub if the number of USB devices in the system exceeds the number of available USB ports Use a hub if the Dionex AS DV is more than 5 meters 16 ft from the P...

Page 106: ...e socket outlet is located near the Dionex AS DV and is easily accessible Operation at AC input levels outside of the specified operating voltage range may damage the Dionex AS DV DANGER D ÉLECTROCUTION Pour éviter toute électrocution il faut utiliser une prise de courant avec prise de terre Ne l utilisez pas et ne le branchez pas au secteur C A sans utiliser de branchement mis à la terre Le cordo...

Page 107: ...unning If the icon is gray the Instrument Controller Service is running idle If the icon is crossed out in red the Instrument Controller Service is not running Right click the icon and click Start Chromeleon Instrument Controller The icon changes to gold to indicate that the Instrument Controller Service is starting 4 If Chromeleon 6 8 is installed check the Server Monitor icon on the Windows task...

Page 108: ...tomatically and click Next It is not necessary to insert a hardware installation disk B 8 2 Configuring the Dionex AS DV in Chromeleon 1 Start the Chromeleon 7 Instrument Configuration Manager or the Chromeleon 6 8 Server Configuration program For Chromeleon 7 click Start on the Windows taskbar and click All Programs Chromeleon 7 Instrument Configuration Manager For Chromeleon 6 8 click Start on t...

Page 109: ... be used To use the valve as an injection valve click Injection To use the valve for another function for example a bleed valve click Auxiliary 5 Change other options as required for your system For information about the other options click the Help button 6 When the configuration is complete select File Save Installation 7 Exit the program Figure B 8 Dionex AS DV Properties Dialog Box Options Tab...

Page 110: ...e Chromeleon 7 client click Start All Programs Chromeleon 7 Chromeleon 7 2 To display the AS DV ePanel click the Instruments category bar on the Console Click your instrument in the Navigation Pane Chromeleon 7 connects to the instrument and displays the ePanel Set Click the AS DV tab see Figure B 9 Figure B 9 Example Chromeleon 7 ePanel Set Dionex AS DV ePanel ...

Page 111: ... and select All Programs Chromeleon Chromeleon 2 To display the panel tabset select View Default Panel Tabset or click the Default Panel Tabset toolbar button 3 To display the Dionex AS DV Control panel click the AS DV tab see Figure B 10 Figure B 10 Example Chromeleon 6 8 Panel Tabset Dionex AS DV Control Panel ...

Page 112: ...eon 6 8 click the Position A and then the Position B button You should hear the valve actuate as it changes position 4 If the carousel or valve do not respond check the Chromeleon audit trail for error messages For troubleshooting information refer to Section 4 1 in this manual or to the Chromeleon Help B 8 5 Setting Up the Dionex AS DV for Simultaneous Injections The simultaneous injection featur...

Page 113: ...s allows the Dionex AS DV to operate without Chromeleon control This appendix provides the following information A description of the Dionex AS DV TTL input control function see Section C 1 Instructions for connecting the Dionex AS DV relay output and TTL input see Section C 2 Example connections to a previous generation of Dionex ion chromatograph and instructions for controlling the Dionex AS DV...

Page 114: ... the inject position until after the load cycle is complete Several factors including vial size and delivery speed determine the time interval between the start of the load cycle and injection of the sample Table C 1 lists the times required to complete four types of load cycles Use these times to determine how long to wait after triggering TTL IN before switching the injection valve to inject Eac...

Page 115: ...o four types of signals The default control mode normal edge is compatible with the output signals provided by Dionex products If the device connected to the Dionex AS DV does not send a normal edge signal select the appropriate control mode Refer to the documentation provided with the controlling device and the information below to determine the correct mode Normal Edge In normal edge operation t...

Page 116: ...rates identically to the normal pulse mode except that the positive and negative edges are reversed in function Selecting the Control Mode 1 Start the Chromeleon 7 Instrument Configuration Manager or the Chromeleon 6 8 Server Configuration program For Chromeleon 7 click Start on the Windows taskbar and click All Programs Chromeleon 7 Instrument Configuration Manager For Chromeleon 6 8 click Start ...

Page 117: ...12 107 The Device Configuration dialog box appears see Figure C 2 4 Select the TTL input control mode required by the controlling device from the Mode list Figure C 2 Device Configuration Dialog Box Selecting the TTL Input Control Mode ...

Page 118: ...t the wire into the plug and use a screwdriver to tighten the locking screw 3 Plug the connector into the 4 pin connector on the Dionex AS DV rear panel see Figure C 1 4 Connect the wires from the Dionex AS DV connector plug to the appropriate connector pins on the controlling device Additional connector plugs are provided with other Dionex products NOTE Check the polarity of each connection Conne...

Page 119: ... IC20 Ion Chromatograph is used to control TTL IN on the Dionex AS DV To control the Dionex AS DV set up a Dionex IC20 method that includes timed events for triggering TTL IN on the autosampler Figure C 5 illustrates the timed events in an example method Figure C 4 Example TTL Input Connection Dionex AS DV Connected to Dionex IC20 RLY 1 OUT TTL 1 OUT RLY 2 OUT TTL 2 IN TTL 1 IN TTL 2 OUT TTL 4 IN ...

Page 120: ...Use one of the following methods to control the relay output states execute direct control commands or include the commands in an instrument method or program Refer to the following instructions Relay loads in excess of 200 mA or with included power supplies over 5 VDC may damage the relay drivers on the CPU INIT 0 00 2 7 3 0 10 0 Timed Events Min Download IC20 Method Send signal to start AS DV lo...

Page 121: ...e click Sampler_Relay 3 In the right pane click the Commands tab 4 Click the required command Closed or Open see the example in Figure C 6 Controlling the Relay Output Directly Using Chromeleon 6 8 1 Open the panel tabset see Section 3 4 2 and press the F8 key The Commands dialog box appears 2 Expand the list of commands under Sampler and then under Sampler_Relay Figure C 6 Chromeleon 7 Command Wi...

Page 122: ...Dionex AS DV Operator s Manual 112 Doc 065259 05 10 12 3 Execute the required command see the example in Figure C 6 Figure C 7 Chromeleon 6 8 Commands Dialog Box Example Relay Control Command ...

Page 123: ... 05 10 12 113 Controlling the Relay Output with an Instrument Method or Program To add relay output control commands to a Chromeleon 7 instrument method use the Script Editor see Figure C 1 Figure C 1 Chromeleon 7 Script Editor ...

Page 124: ...c 065259 05 10 12 In Chromeleon 6 8 you can enter the commands on the Relay and State Devices Options page in the Program Editor or Program Wizard see Figure C 8 Figure C 8 Chromeleon 6 8 Program Wizard Example Relay Output Control Command ...

Page 125: ... 5 mL Pkg of 250 039528 Caps plain no filters 5 0 mL Pkg of 250 079797 Caps plain no filters 0 5 mL Pkg of 250 068947 Tray for 5 0 mL vials 1 068948 Tray for 0 5 mL vials 1 071901 Adapters for 0 5 mL vials Pkg of 50 037987 Tool vial cap insertion 1 Tubing and Fittings 071575 Dionex AS DV Sampling Tip Kit 2 5 cm 1 in 074449 Fitting bolt 10 32 1 074373 Fitting ferrule double cone 1 Kits 068920 6 Por...

Page 126: ...Dionex AS DV Operator s Manual 116 Doc 065259 05 10 12 954745 Fuse IEC 127 rated 3 15 A 1 Part Number Item Quantity ...

Page 127: ...lumbing 92 93 See also High pressure valves C Cables Power cord 84 USB 13 95 Carousel Description 8 Loading 25 Release button 7 9 Removing 70 Carousel Error Counter 20 Carousel LED 8 Caution icon 3 4 CE mark 6 cETLus mark 6 Chromeleon 1 AS DV device driver installation 97 AS DV Properties dialog box 99 Audit trail 8 57 Configuring the AS DV in 98 Connecting the AS DV to the PC 95 Connecting to the...

Page 128: ...eaks detected 66 F Facility requirements 87 Filters vial cap 9 Fitting replacement 69 Flow schematics High pressure valves 16 Flush Factor 32 Front interior 11 Accessing 11 Front view 7 Fuses 13 Replacing 84 Requirements 85 H High pressure valves 11 Configuring as a bleed valve 17 99 Configuring as an auxiliary valve 99 Configuring as an injection valve 99 Configuring in Chromeleon 99 Controlling ...

Page 129: ...nce 56 Messages Error 58 Safety 3 N Needle Error Counter 20 Normal edge TTL input control 105 Normal pulse TTL input control 106 O Operating features Front interior 11 Front view 7 Rear panel 12 Operating principles 14 High pressure valves 15 Rinsing 15 Operating temperature 85 Optional high pressure valves 11 Overlap Mode 53 P Parameters Chromeleon commands 19 PGM file See Programs Chromeleon Phy...

Page 130: ...p 14 Replacing 74 Sensors 8 Sequence Wizard Chromeleon 36 Sequences Chromeleon 36 Service procedures 69 Fuse replacement 84 High pressure valve pod replacement 81 Manually raising sampling head 77 Sampling tip replacement 74 Tubing and fittings replacement 69 Ship Kit 2 Simultaneous injections 102 Spare parts list 115 Specifications Electrical 85 Environmental 85 Physical 85 Sample vials 86 Sampli...

Page 131: ...Actuation Counter 20 Valves See High pressure valves Vial adapters for 0 5 mL vials 10 Vial caps 9 Cleaning 22 Filters 9 Insertion tool 9 Installing 24 Vial Process Counter 20 Vial sensors 8 Vial tray 10 Vials 1 9 Cleaning 21 Filling 23 Preparing 21 Specifications 86 W Warning icon 3 4 Warranty voiding 69 Weight 85 Wellness 19 ...

Page 132: ...Dionex AS DV Operator s Manual Index 6 Doc 065259 05 10 12 ...

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