Prepare samples using MicroAmp
™
tube strips with
attached caps
The following procedure describes how to properly load and seal
MicroAmp tube strips with attached caps using the MicroAmp 96-
well tray/retainer set. For a visual demonstration, watch the video
"How to use adapter tray retainers" by visiting
.
1.
Separate the
blue
tray from the retainer by squeezing the
release catch as indicated in the graphic.
1
2
3
1
1
Release catch
2
MicroAmp
™
96-Well Retainer
3
MicroAmp
™
96-Well Tray
2.
Place the
blue
tray on the splash-free 96-well base.
Note:
Only the bottom tray is used for tubes with attached
caps.
3.
Load the tube strips with attached caps into the tray.
4.
Pipette the reaction mixture into the tubes.
5.
Seal the cap strips using the rocking capping tool:
a.
Slip your fingers through the handle with the holes in the tool
facing down for domed caps and with the holes facing up
for optical caps.
b.
Align the tool over the first eight caps in a row.
c.
Rock the tool back and forth a few times to seal the caps.
d.
Repeat for all remaining rows.
6.
Remove the
blue
tray containing the sealed tube strips from
the 96-well base and place the tray and sealed tube strips into
the instrument.
1
MicroAmp
™
8-Tube Strip with Attached Caps (0.2-mL)
2
MicroAmp
™
96-Well Tray
3
MicroAmp
™
Splash Free 96-Well Base
Seal tube strips with cap strips
IMPORTANT!
Apply significant downward pressure on the sealing
tool in all steps to form a complete seal on top of the tubes.
1.
Align and place the cap strips on the tubes.
2.
Seal the cap strips using the rocking capping tool:
a.
Slip your fingers through the handle with the holes in the tool
facing down for domed caps and with holes facing up for
optical caps.
b.
Align the tool over the first eight caps in a row.
c.
Rock the tool back and forth a few times to seal the caps.
d.
Repeat for all remaining rows.
Prepare samples using MicroAmp
™
Reaction Tubes
The following procedure describes how to properly load and seal
MicroAmp
™
individual tubes with attached caps and the MicroAmp
™
96-well tray for VeriFlex systems. For a visual demonstration, watch
the video "How to use adapter tray retainers" by visiting
.
1.
Set the
black
tray on a 96-well base.
2.
Place the reaction tubes in the
black
tray.
1
2
3
1
MicroAmp
™
Reaction Tube with Cap (0.2-mL)
2
MicroAmp
™
96-Well Tray for VeriFlex
™
Blocks
3
MicroAmp
™
Splash Free 96-Well Base
3.
Pipette the reaction mixture into the reaction tubes.
4.
Cap the tubes.
5.
Remove the
black
tray with sealed reaction tubes from the 96-
well base and place the tray and sealed tubes into the
instrument.
4
VeritiPro
™
Thermal Cycler Quick Reference
