Thermo Scientific Applied Biosystems QuantStudio 6 Pro, User Manual

Page: 58 / 241

Add or remove steps
Add a PCR step or remove a PCR step.
1.
In the Method tab, tap Edit
4
Advanced
4
Manage steps .
Note: Use or to navigate through the method if it is not all displayed.
2.
Tap Add step , then tap (Add) in the location of the step to be added.
3.
Enter the temperature and time, then tap Enter .
4.
Tap Remove step , then tap (Remove) in the appropriate step.
5.
Tap Done .
The Method tab is displayed with the updated method.
Add or remove stages
Add a PCR stage or remove a PCR stage.
1.
In the Method tab, tap Edit
4
Advanced
4
Manage stages .
Note: Use or to navigate through the method if it is not all displayed.
2.
Tap Add Stage , then tap (Add) in the location of the stage to be added.
3.
Tap the type of stage.
•
Hold
•
Pre-Read
•
Post-Read
• Infinite
•
PCR
•
Melt Curve
The new stage is added. The time and temperature must be edited as part of the method (see “Edit
the method”
on page 57 ).
4.
Tap Remove stage , then tap (Remove) .
5.
Tap Done .
Add or remove data collection locations
1.
In the Method tab, tap Edit
4
Advanced
4
Data collection location .
Note: Use or to navigate through the method if it is not all displayed.
Chapter 4
Edit a plate before starting a run
Options to edit the method of a plate file
4
58
QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio ™ 7 Pro Real-Time PCR System User Guide