Slide preparation and operation
The Countess
™
3 and 3 FL cell counters accept disposable Countess
™
Cell Counting Chamber Slides
and glass Countess
™
Reusable Slides on interchangeable, slide-specific carriers.
Recommendations
To obtain the best results, follow these recommendations:
• Ensure that the cell sample is homogeneously mixed.
• The measurement range extends from 1 × 10
4
to 1 × 10
7
cells/mL, but the optimal range is 1 × 10
5
to 4 × 10
6
cells/mL.
• For accurate results in cell viability assays, ensure that the counting area is covered with the cell
suspension and count the cells immediately after staining per the assay protocol.
• Do
not
press the optical surfaces of the chamber slides. Hold the slides by the edges.
• Take care to avoid forming bubbles in the sample.
Load Countess
™
Cell Counting Chamber Slide
1.
Prepare the sample by adding 10 µL of your cell suspension to 10 µL of 0.4% trypan blue stain (for
brightfield only). Mix the sample mixture well by pipetting it up and down a few times.
2.
Gently pipet 10 µL of the sample into the half moon-shaped sample loading area. The sample is
loaded into the chamber through capillary action.
4
20
Countess
™
3 FL Automated Cell Counter User Guide
Summary of Contents for AMQAF2000
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