Thermo Scientific Accela UV/Vis Plus, Hardware Manual

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4
Troubleshooting
Detector-Related Problems
60 Accela UV/Vis Detector Hardware Manual Thermo Scientific
No peaks, or peaks
much smaller than
expected Incorrect wavelength setting Check the wavelength setting. Make sure the
correct file is selected.
Lamp not on or defective Make sure the lamp is lit. Run the detector
diagnostic tests to check the lamp. Replace the
lamp if necessary. See
“Lamp Maintenance” on
page 48 .
Integrator input and output voltage not
matching Verify that the integrator is connected to the
appropriate analog output connections on the
detector’s back panel. See
“Connecting the
Analog Outputs” on page 34 .
Check the attenuation setting on the integrator.
Insufficient sample reaching the detector Check the entire chromatographic system for
leaks. Check the sample injection volume.
Broad tailing peaks Excessive rise time Lower the rise time selection.
Poor connection at flow cell inlet Check the end of the inlet tubing for a clean, flat
surface free of obstructions.
Detector not
powering up Tripped circuit breaker at power outlet Reset the circuit breaker.
Blown detector fuse Replace the fuse. See “Replacing the Fuses” on
page 55 .
Incorrect voltage selected Verify that the voltage selector is set for the
correct incoming line voltage. See
“Checking the
Power Setting and Fuses” on page 12 .
Power cord not connected Connect the power cord.
Detector not running
injection Detector not receiving Start signal Check the connection to the Run contacts on the
back panel of the detector.
Comm LED is amber Lost connection to the data system
computer Check the Ethernet connection to the data
system computer.
Run LED is blinking
amber An error occurred during a run Check the software to determine the nature of
the error, or begin the run again.
Lamps LED is amber Both lamps are off Turn on the lamps and allow 1.5 hours for
warm-up.
Table 6. Detector-related problems (Sheet 3 of 3)
Symptom Cause Remedy