Pierce
™
Peptide Desalting Spin Columns
Catalog Numbers
89851 and 89852
Doc. Part No.
2162704
Pub. No.
MAN
00
17280
Rev.
A.0
Contents
Product
Cat. No.
Contents
Storage
Pierce
™
Peptide Desalting Spin Columns
89852
25 spin columns containing 20 mg of resin in a
1:1 water/DMSO slurry
Store at 2-8°C.
89851
50 spin columns containing 20 mg of resin in a
1:1 water/DMSO slurry
Product description
The Thermo Scientific
™
Pierce
™
Peptide Desalting Spin Columns are optimized for desalting 5 µg to 5 mg of peptide sample from protein digests.
The polymer-based hydrophobic resin contained in the column is stable from pH 1-14 and binds peptide samples with excellent recovery (typical
recovery is >90%) under reversed phase conditions. Columns can also be used for removal of excess hydrolyzed/quenched TMT reagents after
labeling to enable efficient and reliable downstream LC-MS analysis.
Procedure summary
Proteolytic digests of proteins extracted from cells or tissues are loaded onto an equilibrated desalting spin column. Peptides are bound to the
hydrophobic resin under acidic aqueous conditions and desalted by washing the column with acidified water by low-speed centrifugation. A
solution with acidified organic solvent (0.1% TFA, 50% acetonitrile) is then applied to the column to elute bound peptides by centrifugation.
Additional materials required
• Trifluoroacetic acid (TFA) (Product No. 28904)
• Acetonitrile (ACN), LC-MS Grade (Product No. 51101)
• Water, LC-MS Grade (Product No. 51140)
• Methanol (MeOH), Optima
™
LC-MS Grade (Fisher Scientific, Product No. A456-1)
• Thermo Scientific
™
Pierce
™
Low Protein Binding Microcentrifuge Tubes, 2.0 mL (Product No. 88379 or 88380)
• Microcentrifuge with adjustable rotor speed up to 7,000 × g
• Vacuum centrifuge
Additional information
• Do not exceed the recommended centrifugation speeds as this may damage the column frit. Damaged frits may result in resin leakage, leading
to sample loss and/or damage to the LC system.
• Use low protein-binding microcentrifuge tubes to ensure maximum sample recovery.
• Avoid sample contamination and direct skin contact with solvents and chemicals. Always wear gloves when handling the spin columns and
samples.
USER GUIDE
For Research Use Only. Not for use in diagnostic procedures.
