Thermo Scientific 4642010 Instructions For Use Manual Download Page 1

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Finnpipette

Single Channel

Variable & Fixed Volume

Multichannel

Instructions for Use

操作指南

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Summary of Contents for 4642010

Page 1: ...1 Finnpipette F2 Single Channel Variable Fixed Volume Multichannel Instructions for Use 操作指南 Us er Ma nu al ...

Page 2: ...nd safe use The CE mark covers the system containing the CE marked Finnpipette F2 pipette and the CE marked Finntips Product specifications are subject to change without prior notice Finnpipette and Finntip are registered trademarks of Thermo Fisher Scientific Oy 本产品符合欧盟指令98 79 EC的标准并标有CE标志如须依据该指令使用本移液器使用者还应阅读 www thermofisher com 上的其它有关信息或者通过 info pipettes thermofisher com 邮箱联系制造商以确保正确安全地使用...

Page 3: ...PTION 4 PACKAGE 5 SAFETY 5 PIPETTE OPERATION 6 PIPETTING TECHNIQUES 6 CALIBRATION AND ADJUSTMENT 8 MAINTENANCE 12 TROUBLE SHOOTING 14 SPARE PARTS 27 36 目录产品简介 15 包装 16 安全 16 移液器的操作 17 移液方法 17 校准与调节 19 维护保养 23 故障检修 25 备件 27 36 ...

Page 4: ... Volume Range Color code Finntip 4652000 1 µl dark blue Flex 10 10 20 50 4652010 5 µl dark blue Flex 10 10 20 50 4652020 10 µl dark blue Flex 200 250 Univ 200 Ext 300 Flex 300 4652130 20 µl dark blue Flex 200 250 Univ 200 Ext 300 Flex 300 4652030 25 µl dark blue Flex 200 250 Univ 200 Ext 300 Flex 300 4652040 50 µl dark blue Flex 200 250 Univ 200 Ext 300 Flex 300 4652050 100 µl dark blue Flex 200 2...

Page 5: ...e to health Follow general procedures for hazard prevention and safety instructions e g wear protective clothing eye protection and gloves For use and waste disposal of hazardous e g radioactive and potentially infectious material follow the safety instructions and general laboratory practice The pipette and tips are not intended for in vivo use Do not use the pipette for pipetting any liquid to b...

Page 6: ...To increase the delivery volume turn the push button counterclockwise To decrease the delivery volume turn it clockwise 2 Make sure that the desired delivery volume clicks into place 3 Do not set volumes outside the pipette s specified volume range Using excessive force to turn the push button outside the range may jam the mechanism and eventually damage the pipette Safety Label You can mark the p...

Page 7: ...uid touching it against the edge of the reservoir to remove excess liquid 3 Deliver the preset volume by gently depressing the push button to the first stop Hold the push button at the first stop Some liquid will remain in the tip and this should not be included in the delivery 4 The remaining liquid should either be discarded with the tip or pipetted back into the container Repetitive technique T...

Page 8: ...e 3 water conforming ISO 3696 Tests are done in a draft free room at a constant 0 5 C temperature of water pipette and air between 15 C to 30 C The relative humidity must be above 50 Especially with volumes under 50 µl the air humidity should be as high as possible to reduce the effect of evaporation loss Special accessories such as the evaporation trap are recommended Procedure to check calibrati...

Page 9: ...00 40 0 8 0 15 0 3 0 1 10 ml 10000 60 0 0 6 30 0 0 3 1000 60 0 6 0 30 0 3 0 Finnpipette F2 Fixed Volume Single Channel Fixed Volume Inaccuracy Imprecision µl µl s d µl cv 1 0 050 5 00 0 050 5 00 5 0 125 2 50 0 075 1 50 10 0 120 1 20 0 080 0 80 20 0 20 1 00 0 10 0 50 25 0 50 2 00 0 20 0 80 50 0 50 1 00 0 20 0 40 100 0 80 0 80 0 30 0 30 200 1 60 0 80 0 60 0 30 250 4 00 1 60 1 50 0 60 500 4 00 0 80 1...

Page 10: ...0 Flex 200 10 0 30 3 00 0 10 1 00 20 200 µl 200 1 2 0 60 0 4 0 20 Flex 200 20 0 36 1 80 0 14 0 70 100 1000 µl 1000 6 0 0 60 2 0 0 20 Flex 1000 100 1 0 1 00 0 6 0 60 0 5 5 ml 5000 25 0 0 50 10 0 0 20 5 ml 500 10 0 2 00 4 0 0 80 1 10 ml 10000 50 0 0 50 20 0 0 20 10 ml 1000 20 0 2 00 8 0 0 80 Finnpipette F2 Fixed Volume Single Channel Fixed Volume Inaccuracy Imprecision Factory µl µl s d µl cv calibr...

Page 11: ...rsion factor for µl mg conversion Evaporation loss can be significant with low volumes To determine mass loss dispense water to the weighing vessel note the reading and start a stopwatch See how much the reading decreases during 30 seconds e g 6 mg 0 2 mg s Compare this to the pipetting time from taring to reading Typically pipetting time might be 10 seconds and the mass loss is 2 mg 10 s x 0 2 mg...

Page 12: ...n spring and the O rings with a dry napless cloth 6 Check the tip cone for foreign particles 7 Grease the cleaned parts with the lubricant that comes with the pipette 8 Reassemble the pipette components 0 2 2µl 0 5 5µl 1 10 µl First slide spring 22 o ring support 23 and o ring 24 on the tube 21 With the 0 2 2µl model insert the tube 27 into the tube 21 Then slide the spring 13 spring support 16 an...

Page 13: ... 10µl The tip cones cannot be serviced please replace if necessary 30 300 µl 10 100µl 5 50 µl Open the tip cone by carefully releasing the cover ring from its snap joint with the screwdriver Remove all the parts from the tip cone Clean all the parts If needed replace the o rings Take one piston Slide the spring 33 cover ring 32 larger hole spring 34 support ring 35 o ring 37 bigger 5 50µl 10 100µl...

Page 14: ...pipette is designed to allow easy in lab service If you would prefer to have us or your local representative service your pipette please make sure that the pipette has been decontaminated before you send it to us Please note that the postal authorities in your country may prohibit or restrict the shipment of contaminated material by mail Service Instructions for Multichannel Pipette Tip Cones To e...

Page 15: ...0 250 Univ 200 Ext 300 Flex 300 4652130 20 µl dark blue Flex 200 250 Univ 200 Ext 300 Flex 300 4652030 25 µl dark blue Flex 200 250 Univ 200 Ext 300 Flex 300 4652040 50 µl dark blue Flex 200 250 Univ 200 Ext 300 Flex 300 4652050 100 µl dark blue Flex 200 250 Univ 200 Ext 300 Flex 300 4652140 200 µl dark blue Flex 200 250 Univ 200 Ext 300 Flex 300 4652060 250 µl dark blue Flex 1000 1000 1000 Ext 46...

Page 16: ...全此符号代表注意用途此设备的用途是用来移取范围在0 2 μl 到 10 ml之间的液体 Finnpipette F2 以及 Finntips的设计是作为分析系统的一部分为那些负责校验系统的终端客户可以得到可靠及安全的结果对健康的危害对于危害的预防以及安全说明请遵循一般流程例如穿防护服配带防护眼镜及手套对于有害物质的使用以及废弃物的处理例如带有辐射的或者有潜在传染性的物质请遵循相关的安全说明或者一般实验室规范移液器以及吸嘴不能在体内使用请不要使用移液器吸取液体后再注射入人体内请不要在弹出吸嘴时将吸嘴对着任何人 Finnpipette F2 的使用者需要具备实验室操作技术并通过培训的人员在使用移液器及吸嘴之前或者使用过程中必须先阅读使用说明 F2 移液器可以在 4 C 到 40 C之间进行使用错误的移液结果由于以下因素可能会对性能造成偏差 a...

Page 17: ...想要将之废弃请与当地环保部门联系如果想要了解更多信息请联系我们info pipettes thermofisher com 或者联系当地Thermo Fisher Scientific 赛默飞世尔科技代表关于原包装和包装材料请使用您所知道的回收经营者移液器的操作设定移液量 1 使用移液器顶部的按钮设定移液量如需增加移液量应按逆时针方向旋转按钮如需减少移液量应按顺时针方向旋转按钮 2 应确保移液量设定到位且完整读取显示窗口上的读数 3 勿将液量设定在移液器指定的容量允许范围以外使用蛮力过度旋转按钮可能会导致卡壳并最终使移液器受损安全标签可在安全标签上记下移液器的用途使用者姓名缩写校准日期等可用较细的针将标签取下用笔在标签上书写然后将标签放回原处吸嘴的卸除为防止污染移液带有管嘴推出器如需卸下吸嘴应将移液器指向适当的废液槽然后用拇指...

Page 18: ...按下按钮至第一停点即可获得事先设定的液量在第一停点处按住按钮吸嘴内会有部分残留液体不应将其计入实际的移液量 4 应将残留液体与吸嘴一起丢弃或将其重新注入原容器内反复移液方法反复移液方法是用于重复提取相同容量液体的一种快速简单的方法将用于移液的液体注入干净的试剂容器内 1 按下按钮到底 2 将吸嘴探入容器内液体表面下约1 cm 处然后慢慢释放按钮这样做可以将液体吸入吸嘴将吸嘴从液体中取出并靠在容器边缘以去除多余的液体 3 轻轻按下按钮至第一停点即可获得事先设定的液体容量在第一停点处按住按钮吸嘴内会有部分残留液体不应将其计入实际的移液量 4 可通过重复第3和第4步操作继续进行移液非均质液样的移液操作全血移液法使用正向移液方法的第1和第2步骤将血液吸入吸嘴使用清洁干燥的薄纸仔细将吸嘴擦干 1 将吸嘴伸入试剂内并按动按钮至第一停点同时应保证吸嘴...

Page 19: ...室内进行并应使水移液器和空气温度恒定在15 C与30 C之间误差在 0 5 上下相对湿度必须大于50 特别是液量在50µl以下时空气湿度越高越好以便减少蒸发作用对液量产生的影响建议使用专门的辅助仪器如加湿器校准检查的步骤应检查移液器的最大液量标定液量和最小液量新的吸嘴应预湿3 5次并对最大和最小两种液量各进行十次移液操作每次移液 Ex 时都应对移液器进行调节以获得所需的液量建议使用正向移液方法允许的最大误差值专为正向操作方法而设计建议对多道移液器的每一个通道都进行校准但是仅去校准两边最外侧的通道是比较合适的校准方法在校准之前请先通过使用中间的某一个通道来进行调整检查建议使用下表中ISO8655的校验标准或者用户自定义标准步骤 1 进行一系列10次最小液量的移液操作 2 进行一系列10次最大液量的移液操作 3 计算以上两个系列操作的错...

Page 20: ... 40 0 8 0 15 0 3 0 1 10 ml 10000 60 0 0 6 30 0 0 3 1000 60 0 6 0 30 0 3 0 Finnpipette F2 固定式量程单道固定液量错误率不精确率 µl µl s d µl cv 1 0 050 5 00 0 050 5 00 5 0 125 2 50 0 075 1 50 10 0 120 1 20 0 080 0 80 20 0 20 1 00 0 10 0 50 25 0 50 2 00 0 20 0 80 50 0 50 1 00 0 20 0 40 100 0 80 0 80 0 30 0 30 200 1 60 0 80 0 60 0 30 250 4 00 1 60 1 50 0 60 500 4 00 0 80 1 50 0 30 1000 8 00 0 80 3 00 0 30 2000 16 0 ...

Page 21: ... 0 30 3 00 0 10 1 00 20 200 µl 200 1 2 0 60 0 4 0 20 Flex 200 20 0 36 1 80 0 14 0 70 100 1000 µl 1000 6 0 0 60 2 0 0 20 Flex 1000 100 1 0 1 00 0 6 0 60 0 5 5 ml 5000 25 0 0 50 10 0 0 20 5 ml 500 10 0 2 00 4 0 0 80 1 10 ml 10000 50 0 0 50 20 0 0 20 10 ml 1000 20 0 2 00 8 0 0 80 Finnpipette F2 固定式量程单道固定液量错误率不精确率工厂用校 µl µl s d µl cv 验吸嘴 1 0 040 4 00 0 040 4 00 Flex 10 5 0 070 1 40 0 070 1 40 Flex...

Page 22: ...使用中间的某一个通道来进行调整检查 1 将工具放入手柄上端的校准螺开口内 2 顺时针旋转辅件即可增加容量逆时针旋转可减少容量 3 调节后应按上述说明进行校准检查计算公式质量与体积的转换 V w e x Z V 体积 µl w 质量 mg e 蒸发量 mg Z mg µl 转换中的换算因数蒸发量对较小的液量而言有重要的影响需确定蒸发量可将水注入量杯记下读数然后开始按下计时器观察读数在30秒内的变化即 6 mg 0 2 mg s 将此数据与移液操作中从配衡到读数间所需时间进行比较对于上述情况一般移液操作可能需要10秒钟时间蒸发量为2 mg 10 s x 0 2 mg s 如果在容器上加盖了盖子或使用了加湿器则一般不必再对蒸发量进行修正因数Z用于在试验温度和压力的条件下将水的质量换算成体积在22 C和95 kPa的条件下其值通常为1 0032 µl m...

Page 23: ... 活塞弹簧和O形环 6 检查管嘴连件是否留有异物 7 使用移液器配套的润滑油为已清洁的部件上油 8 重新装好移液器的各个组件 0 2 2ul 0 5 5ul和1 10ul 首先将弹簧22 O型圈23和24套在管子21上面把0 2 2ul的活塞插入到管子27中然后再插到管子21中然后弹簧13 弹簧支撑16和管子17和18 大的O型圈19和小的密封圈20 套在活塞上面用手向后收缩活塞的弹簧和弹簧支撑件16和管子21 把住弹簧并小心的将活塞装进管嘴并松开弹簧 2 20 µl和5 50 µl 将弹簧13 弹簧垫圈16 导管17和18 大O形环19和小O 形环20重新套在活塞上用手指挤压活塞和弹簧垫圈16间的弹簧并将大O 形环19 小O形环20 弹簧垫圈21和弹簧22 直径比弹簧垫圈21小套在活塞上继续挤压弹簧小心将整个部件装入管嘴连件然后松开弹簧 10 10...

Page 24: ...50ul 用螺丝起子小心地松开盖环然后打开吸嘴连杆取下吸嘴连杆上的所有部件并清洁所有部件必要时更换O形环取下活塞滑动弹簧33 盖环32 大孔弹簧34 垫环35 大O形环37 5 50ul 10 100ul 和小O形环36依次套在活塞上使用移液管包装中随附的润滑油为O形环上油滑动所有吸嘴连杆部件然后闭合盖环 1 10ul 用螺丝起子小心地松开盖环然后打开吸嘴连杆取下吸嘴连杆上的所有部件并清洁所有部件必要时更换O形环取下活塞滑动弹簧33 盖环32 大孔支撑件35 大O形圈36 小O形环37和O形环38 然后依次在O形圈支撑件38上面装上弹簧39 弹簧支撑件40 边缘较尖和O形圈41 使用移液管包装中随附的润滑油为O形环上油滑动所有吸嘴连杆部件然后闭合盖环 11 将活塞杆与活塞一起安装和吸嘴连杆安装到盖子里盖好盖子然后用4颗螺丝将盖子闭合...

Page 25: ...液体的移液结果不准确解决方法必须妥善安装清洁管嘴连件后装上新的吸嘴清洁O形环和缸体并上好润滑油应适当润滑更换O形环按说明进行各步骤操作必须妥善安装按说明重新进行校准使用维修工具去锁紧吸嘴连件和外壳按压活塞到第二停的位置多按压几次让润滑油扩散开对该液体重新进行校准注意 Finnpipette 的设计旨在提供实验室内的便利服务如果希望我们或当地办事处能够对您的移液器进行维修请务必在将移液器送给我们之前把它消毒干净请注意您所在国家的邮政机构可能禁止或限制通过邮件递送被污染的物品故障检修下表列出了可能会出现的问题及其解决办法消毒吸嘴座可在121 C 252 F 2个绝对大气压下进行高温消毒35分钟必要时可使用蒸汽消毒包吸嘴座经高温消毒后必须经过至少两个小时时间冷却至室温才可使用移液前务必确保仪器的干燥建议在每次消毒之后...

Page 26: ... 1 0032 1 0033 1 0033 1 0033 22 5 1 0032 1 0032 1 0033 1 0033 1 0034 1 0034 1 0034 23 0 1 0033 1 0033 1 0034 1 0034 1 0035 1 0035 1 0036 23 5 1 0034 1 0035 1 0035 1 0036 1 0036 1 0036 1 0037 24 0 1 0035 1 0036 1 0036 1 0037 1 0037 1 0038 1 0038 24 5 1 0037 1 0037 1 0038 1 0038 1 0039 1 0039 1 0039 25 0 1 0038 1 0038 1 0039 1 0039 1 0040 1 0040 1 0040 25 5 1 0039 1 0040 1 0040 1 0041 1 0041 1 0041 ...

Page 27: ...2 42 42 2 2211810 Fix 1 10ml 2 2211590 10ml 2 2211580 5ml 3 2213260 1 10ml 3 2213250 0 5 5ml 3 2213450 Fix 10ml 3 2213440 Fix 5ml 3 2213430 Fix 3ml 3 2213420 Fix 2ml 12 1033050 10ml 12 1030230 5ml 42 2211670 1 10ml 42 2211920 Fix 6 10ml 42 2211660 0 5 5ml 42 2211910 Fix 2 5ml 2 3 2 Cap 3 Handle 12 O ring 42 Tip Cone assy ...

Page 28: ... 2211550 20 200µl 3 2213230 20 200µl 3 2213120 Fix 200µl 17 1030160 42 2211630 10 100µl 25 µl Fixed 50 µl Fixed 100 µl Fixed 2 2211550 10 100µl 3 2213220 10 100µl 3 2213380 Fix 100µl 3 2213370 Fix 50µl 3 2213360 Fix 25µl 17 1030510 42 2211620 20 200µl 200µl Fixed 10 100µl 25 50 100µl Fixed 12 14 15 13 11 10 16 12 14 15 13 11 10 16 100 1000µl 250 500 1000µl Fixed 2 Cap 3 Handle 17 O ring 42 Tip Con...

Page 29: ...Micro 3 2213270 5 50µl 3 2213300 5 50µl Micro 19 1030500 20 1033060 42 2213010 5 50µl 42 2212990 5 50µl Micro 1 10µl 1 10 µl Micro 10 µl Fixed 2 2211550 1 10µl 2 2211520 1 10µl Micro 3 2213190 1 10µl 3 2213180 1 10µl Micro 3 2213350 Fix 10µl 19 1030380 20 1030060 24 1030170 42 2211540 42 2211510 1 10µl Micro 42 2211510 Fix 10µl 1 10µl 1 10µl Micro 10µl Fixed 12 13 12 13 11 10 11 10 14 15 17 16 18 ...

Page 30: ...170 42 2211900 0 2 2 µl 1 µl Fixed 2 2211520 0 2 2µl 3 2213160 0 2 2µl 3 2213330 Fix 1µl 24 1030170 28 2205730 42 2211890 12 13 12 13 11 10 11 10 14 15 17 16 18 21 22 14 15 17 16 18 19 21 20 22 23 0 2 2 µl 1 µl Fixed 23 27 2 Cap 3 Handle 19 O ring 20 O ring 24 O ring 28 Sealing Combination 42 Tip Cone assy 19 20 28 42 42 24 24 ...

Page 31: ... 21 22 23 24 42 44 43 45 2 Cap 3 Handle 42 Tip Cone assy 50 Module 50 1 10µl 2 2211520 3 2213180 42 2212770 12 pcs 50 2211260 8 ch 50 2211270 12 ch 10 100µl 2 2211550 3 2213220 42 2212790 12 pcs 50 2211300 8 ch 50 2211310 12 ch 32 31 30 35 34 36 10 100µl 33 42 37 ...

Page 32: ...32 33 30 31 32 34 35 37 36 21 21 22 23 42 15 12 13 19 17 16 18 46 44 43 45 50 2 Cap 3 Handle 42 Tip Cone assy 50 Module 5 50µl 2 2211560 3 2213270 42 2212780 12 pcs 50 2211280 8 ch 50 2211290 12 ch ...

Page 33: ...33 21 21 22 23 15 12 13 19 17 16 18 46 44 43 45 50 2 Cap 3 Handle 42 Tip Cone assy 50 Module 42 33 31 32 34 30 35 36 30 300µl 2 2211680 3 2213280 42 2212800 12 pcs 50 2211320 8 ch 50 2211330 12 ch ...

Page 34: ...34 15 12 13 19 33 17 16 46 30 31 34 37 32 35 36 45 43 44 21 21 22 24 42 18 23 2 Cap 3 Handle 42 Tip Cone assy 50 Module 5 50µl 16 ch 2 2211560 3 2213300 42 2212810 8 pcs 50 2211350 50 ...

Page 35: ...35 15 12 13 19 17 16 46 45 43 44 21 21 22 24 18 23 2 Cap 3 Handle 42 Tip Cone assy 50 Module 31 37 42 33 1 10µl 16 ch 2 2211800 3 2213290 42 2212820 8 pcs 50 2211340 50 Picture 圖片 ...

Page 36: ...1000 µl FP F2 0 5 5 ml FP F2 1 10 ml FP F2 Fixed Volume 1 µl FP F2 Fixed Volume 5 µl FP F2 Fixed Volume 10 µl FP F2 Fixed Vulume 20 µl FP F2 Fixed Volume 25 µl FP F2 Fixed Volume 50 µl FP F2 Fixed Volume 100 µl FP F2 Fixed Volume 200 µl FP F2 Fixed Volume 250 µl FP F2 Fixed Volume 500 µl FP F2 Fixed Volume 1000 µl FP F2 Fixed Volume 2000 µl FP F2 Fixed Volume 3000 µl FP F2 Fixed Volume 5000 µl FP ...

Page 37: ...l 10ml 10ml Flex Ext 10 Micro 10 Flex micro 20 Micro 50 Micro 10 Univ 20 Univ 30 Univ 30 Flex 100 Ext 100 Univ 100 Flex 200 Ext 200 Flex 200 Univ 300 300 Flex 1000 Ext 1000 1000 Flex 1200 Flex 5 ml 10ml 10ml Flex Ext Finntip compatibility chart 1508760 04 ...

Page 38: ...5x54 rack 9402160 10 ml 1 10 ml 5x24 rack 9402163 10 ml sterile 1 10 ml 5x24 rack 94060970 Flex 10ml Ext 1 10 ml 100 pcs bag 94060973 Flex 10ml Ext sterile 1 10 ml 50 pcs bag Also available in bags Code Finntip Filter Volume Qty 94052000 10 Micro sterile 0 2 10 µl 10x96 rack 94056980 Flex 10 sterile 0 2 10 µl 10x96 rack 94052100 10 sterile 0 5 10 µl 10x96 rack 94052020 20 Micro sterile 0 2 20 µl 1...

Page 39: ...39 ...

Page 40: ...erved All trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Thermo Fisher Scientific Oy Ratastie 2 01620 Vantaa Finland ...

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