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Introducing the System

System Instruments and Peripherals

Thermo Electron Corporation

LOR772 System User Guide

1-3

•

One PerkinElmer PlateStak storage unit

•

One Biotek ELx405 washer

The system uses an Articulated Robot mover controlled by the POLARA
scheduling software, to move microtitre plates between instruments. For
details on the components of the system, see

“System Components”

.

For details on the operation of the instruments, refer to the user guides
supplied with the instrument.

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Summary of Contents for LOR772

Page 1: ...Thermo Electron Corporation Hospital for Sick Children CatX Workstation LOR772 System User Guide ...

Page 2: ...this material including but not limited to the implied warranties of merchantability and fitness for a particular purpose Thermo CRS assumes no responsibility for any errors that may appear in this document Thermo CRS makes no commitment to update nor to keep current the information contained in this document Thermo CRS software products shall remain the property of Thermo CRS Ltd Additional copie...

Page 3: ...nal aids to help you quickly find the topics and information you need Before following instructions in a section read the entire section first This guide consists of the following chapters Introducing the System which introduces the system and provides an overview of its basic components Safe Use of the System which describes safety directives with which you and all operators of the system must be...

Page 4: ...and schedule and perform runs see the POLARA 2 3 user guides Training We offer courses at our facility in Burlington Ontario Canada or on site at your facility For more information contact the Thermo Electron Customer Support Group Contacts You can contact Thermo LAI personnel in any of the following ways Surface Mail Shipping Thermo Electron Corporation Laboratory Automation and Integration 5344 ...

Page 5: ...ter 2 Safe Use of the System 2 1 Built in Safety Features 2 2 Ensuring Safe Use of the System 2 3 Operating the System Safely 2 5 Understanding Warnings and Error Messages 2 6 Warnings 2 6 Error Messages 2 6 Triggering an E Stop 2 7 Adding E Stops to the System 2 9 Chapter 3 Using the System 3 1 System Requirements 3 2 Air Supply 3 2 Electric Power 3 2 User Capabilities 3 2 Bringing the System Onl...

Page 6: ...roubleshooting 5 1 Recovering from a Mover Collision 5 2 Getting Help 5 3 Preparing to Contact Thermo Electron 5 3 Gathering Problem Data 5 4 Capturing System Error Messages 5 5 Capturing Text 5 5 Capturing Graphics 5 6 Contacting Thermo Electron Customer Support 5 7 Index I 1 ...

Page 7: ...lectron Corporation LOR772 System User Guide v Figures Hospital for Sick Children CatX Workstation layout 1 2 The Master Control Panel MCP 1 11 MCP controls 1 11 POLARA provides a graphical user interface 1 13 ...

Page 8: ......

Page 9: ...lectron Corporation LOR772 System User Guide vii Tables Instrument usage by configuration 1 4 Controller input power ratings 1 8 CatX environmental requirements 1 10 Computer and Belkin Port Number mapping 3 6 ...

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Page 11: ...lar and expandable system for high throughput screening This chapter introduces the system and provides an overview of its basic components It includes the following topics System Instruments and Peripherals on page 1 2 System Components on page 1 6 Master Control Panel on page 1 11 POLARA System Software on page 1 13 CRS DataHandler Software on page 1 14 ...

Page 12: ...e following instruments and peripherals Figure 1 1 Hospital for Sick Children CatX Workstation layout One Thermo CRS Regrip station built in to CatX base One Thermo CRS Lidder One Thermo Cytomat 2 incubator One MicroScan MS 3 Bar Code reader One PerkinElmer Evolution P3 liquid handler One PerkinElmer Envision reader One PerkinElmer Multiprobe pipettor note this instrument is not integrated into th...

Page 13: ...age unit One Biotek ELx405 washer The system uses an Articulated Robot mover controlled by the POLARA scheduling software to move microtitre plates between instruments For details on the components of the system see System Components on page 1 6 For details on the operation of the instruments refer to the user guides supplied with the instrument ...

Page 14: ... information for operating the POLARA configuration only To setup a configuration follow these procedures To setup a POLARA configuration 1 If the PlateStak is in the profile selected for your POLARA method change the PlateStak serial port switch so POLARA can communicate with the PlateStak 2 If the Evolution is in the profile selected for your POLARA method ensure that its scripts used in POLARA ...

Page 15: ...e POLARA configuration as described in Chapter 3 Using the System To setup a WinPREP configuration 1 If POLARA is running ensure it is not running methods whose profile includes the PE Evolution and PlateStak 2 Change the PlateStak serial port switch so that WinPREP can communicate with the PlateStak 3 Continue using the WinPREP configuration on the WinPREP computer according to PerkinElmer requir...

Page 16: ...ntainers between instruments Articulated robots consist of linked joints that rotate or slide to change the location of its gripper The C500C controller directs the motion of the robot arm and provides power and safety circuits The controller is built into the base of the CataLyst Express Note The container transport also includes an E Stop circuit into which instruments can be integrated The seri...

Page 17: ...to operate computers in the system LAN Uninterruptible power supplies UPS which provide continuous power to the system If an interruption occurs in the main power supply the UPSs maintain power for up to 30 minutes Guarding consisting of plexiglass barriers that block access to moving parts of the transport For details about Dimension4 components see the following topics Power Input Requirements o...

Page 18: ... require the power inputs listed in Table 1 2 Table 1 2 Controller input power ratings Description Rating Input voltage 110 115 230 VAC 10 Line frequency 50 60 Hz WARNINGThe controller must be grounded The AC cord supplied with the controller provides the proper grounding terminal Do not cut off the third terminal from the AC cord set ...

Page 19: ...nents Thermo Electron Corporation LOR772 System User Guide 1 9 Fuse and Circuit Breaker Requirements Refer to the C500C Controller User Guide or the CataLyst Express User Guide for fuse and circuit breaker requirements of AR controllers ...

Page 20: ... shown in Table 1 3 Table 1 3 CatX environmental requirements Environment Condition Requirement Relative humidity Below 50 non condensing Temperature 10 40 C Ventilation A minimum of 11 5 cm 4 5 in clearance between the grills at the rear of the controller and your enclosure Atmosphere Clean low dust dry office lab environment Installation location for INDOOR use only ...

Page 21: ...Master Control Panel You use the Master Control Panel MCP to control power to the The MCP consists of a small metal box connected to the Figure 1 2 The Master Control Panel MCP Figure 1 3 MCP controls The MCP provides the following system power functions E Stop button Arm status indicators Brake release ...

Page 22: ...n Corporation An EMERGENCY STOP E Stop button that when pressed immediately cuts power to the robot arm but not necessarily to the instruments To understand the Arm status indicators and Brake release button refer to the Using the E Stop Control section in your CataLyst Express User Guide ...

Page 23: ...vides Hospital for Sick Children CatX Workstation users with an easy to use graphical interface for operating and managing the system and for developing and scheduling methods Figure 1 4 POLARA provides a graphical user interface to operate the system For details about using POLARA read the POLARA user guide or choose Contents in the POLARA Help menu to display POLARA s online help ...

Page 24: ...n CRS DataHandler Software The Hospital for Sick Children CatX Workstation system includes a Windows application called DataHandler that reads the data log Before a run in POLARA go to the Site Parameters and ensure the correct validation and results directories are entered See Starting a Run on page 3 7 ...

Page 25: ...For details see the following topics Built in Safety Features on page 2 2 Ensuring Safe Use of the System on page 2 3 Operating the System Safely on page 2 5 Triggering an E Stop on page 2 7 Adding E Stops to the System on page 2 9 CAUTION This manual describes the safe use of Thermo Electron components of the Hospital for Sick Children CatX Workstation system Refer to the manufacturer s instructi...

Page 26: ...onitor window displays information during the progress of a run including error messages and warnings If POLARA detects that operator intervention is required during a run for instance if a non standard container needs to be repositioned or removed it suspends the run and alerts the operator The Hospital for Sick Children CatX Workstation system also includes the following safety features Automati...

Page 27: ...l of it before using a POLARA lab system When using the system you must observe the following safety guidelines Do not allow untrained persons to control the system Any person who is authorized to operate the container transport must be fully trained in container transport safety and the use of container transport motion commands Train any person who uses the laboratory system in the dangers and r...

Page 28: ...ove unexpectedly at high speeds Do not touch automated components when the system is running Never operate the system if you believe any substance has dripped into or otherwise entered the container transport hardware If the system is being serviced or modified or is acting abnormally ensure that it is clearly labeled as Out of Service to prevent accidental use Following any change in lab system h...

Page 29: ... When cleaning the waste chute wear appropriate protective personal equipment such as gloves safety glasses a lab coat and a breathing apparatus Keep loose clothing and long hair away from moving parts Remove jewelry such as rings bracelets and necklaces before operating the system This symbol which is placed at several locations on the system indicates pinch point hazards Avoid placing any part o...

Page 30: ...tem issues a warning when it encounters a condition that should be noted and reported but is not serious enough to warrant suspending the run It alerts operators to a warning in the following ways A warning appears in the Messages tab of the POLARA Monitor window and the Monitor warning status indicator turns yellow Error Messages The system suspends a run when it encounters an error that requires...

Page 31: ...mponents If any are physically damaged recovery is not possible Click Abort in the message box presented by POLARA 2 Verify that it is safe for the run to continue Clean up any spills If any containers have been misplaced or dropped replace them as needed Check the integrity of the run Some processes are extremely sensitive to temperature changes or contaminants If necessary take note of any sampl...

Page 32: ...fe Use of the System Triggering an E Stop 2 8 LOR772 System User Guide Thermo Electron Corporation 5 At the system computer click Retry or any other option that will allow you to proceed The run continues ...

Page 33: ...the System Thermo Electron Corporation LOR772 System User Guide 2 9 Adding E Stops to the System To improve safety within your workcell you can connect additional E Stops Refer to your CataLyst Express User Guide chapter CataLyst Express Ports ...

Page 34: ......

Page 35: ...tion using POLARA However there are some requirements that must be met and tasks that must be performed outside of the POLARA operating environment For details see the following topics System Requirements on page 3 2 Bringing the System Online on page 3 3 Taking the System Offline on page 3 5 Selecting the Computer to Operate on page 3 6 Starting a Run on page 3 7 ...

Page 36: ...ng Users must monitor the site location power supply To halt a run see the Halting a Run section in the Setting Up and Performing a Run chapter of your POLARA User Guide User Capabilities Before operating this system you must be trained in its safe use by Thermo Electron For details on training offered by Thermo Electron see Training on page 2 in the Preface You must also have a thorough understan...

Page 37: ...mputer and all other instrument computers 4 Power on all instruments To test the Emergency Stops 1 Test the E Stop chain by taking the following steps a Press the E Stop button on the Master Control Panel MCP b Inspect the movers All mover power beacons should be OFF 2 Reset the E Stop button by twisting it clockwise until it pops up 3 Press and hold the RESET button on the MCP until it turns gree...

Page 38: ...quire you to perform additional setup of some instruments For details see the setup instructions displayed by POLARA before each run CAUTION If any mover power beacon remains OFF when MOTOR POWER ON button is green do not operate the system Inform all system users that the system is unavailable and contact Thermo Electron Customer Support immediately as described in Contacting Thermo Electron Cust...

Page 39: ...rs from the system 5 Shut down the system and instrument computers 6 Power off each controller by switching the POWER button to its 0 position 7 Turn off the system air supply by turning the air regulator valve counter clockwise to the OFF position CAUTION Do not exit POLARA by shutting it down in the Windows Task Manager Doing so leaves some POLARA mover control components still running CAUTION I...

Page 40: ... and correctly connected to select it See the Belkin documentation for operation details In brief you can use any of following methods to select a computer Port Number Press a front panel button on the Belkin KVM switch Press the keyboard Scroll Lock twice and the Up or Down arrow key to switch to the previous or next Port Number respectively Press the keyboard Scroll Lock twice and a number key t...

Page 41: ... refer to your POLARA Guide To start POLARA 1 On the system monitor use the Belkin switch to select the POLARA computer 2 Use one of these methods to start POLARA In the Start Menu choose Programs CRS Robotics POLARA V2 3 Double click the POLARA 2 3 icon on the Windows desktop Tip You can get help at any time in POLARA by choosing Contents in the POLARA Help menu To setup data validation 1 On the ...

Page 42: ...Enter or change the following information Assay Name Operator Name Output Directory 2 to put a copies of Envision output data files into another directory Choose Browse to navigate to another directory 5 Select Delect original Envision file to delete Envision data files between POLARA runs The files are delected only after they are copied to the output directories specified above 6 Choose OK ...

Page 43: ...he system requires little daily maintenance other than inspection and cleaning For system maintenance see the CataLyst Express User Guide chapter called Maintenance Procedures For additional details see the following topics Lockout Tagout Procedures on page 4 2 Monthly Maintenance on page 4 3 ...

Page 44: ...out unplug the power cords from every instrument and controller For air lockout tagout turn off the air supply by rotating the air regulator valve This also relieves the pressure in the system Apply a lockout tagout boot WARNINGEnsure your laboratory personnel use Lockout and Tagout procedures for air and electrical main connections during maintenance ...

Page 45: ...h test the E Stop chain to ensure that all movers and other components connected to the E Stop chain have their power cut when the E Stop is triggered To test the E Stop chain 1 Take the system off line as described in Taking the System Offline on page 3 5 2 Bring the system online as described in Bringing the System Online on page 3 3 ...

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Page 47: ...ter 5 Troubleshooting For troubleshooting procedures see the Troubleshooting chapter in your CataLyst Express User Guide For capturing problem data and error messages prior to getting help see Recovering from a Mover Collision on page 5 2 Getting Help on page 5 3 ...

Page 48: ...nt in the Defining Motion in AR Workspaces chapter of your POLARA Administrator Guide c Start ash for the instrument where the collision occurred d With a trained user standing by to catch the container open the gripper with the go command e If necessary move the arm away from the instrument so that the gripper is outside the instrument nest Limp the arm or use joint commands very carefully to mov...

Page 49: ...uickly if you can collect crucial information before contacting Thermo Electron Customer Support To prepare to contact Thermo Electron Customer Support 1 Gather problem data For details see Gathering Problem Data on page 5 4 2 E mail any relevant files or information to services labautomation thermo com Include a description or screen captures of any errors or messages reported by the system For d...

Page 50: ...pturing Graphics on page 5 6 2 Try to repeat the problem and capture any error messages that occur Tip If you cannot engage arm power make sure that all devices in your E Stop chain are closed If you still cannot engage arm power it is likely that you have a mechanical problem with the mover 3 Make copies of the POLARA log files are described in your POLARA User Guide in section Making Backup copi...

Page 51: ...e it into a text editor such as Notepad Tip Saving a message as text makes it easier to read The file size is also much smaller than for a graphic screen capture To capture text from CROSnt 1 Right click in the title bar of the CROSnt window You will see the following menu 2 Choose Edit Mark Your cursor changes to a solid cursor Scroll up to the top of the window then click and drag with your mous...

Page 52: ...s Paint to open the Microsoft Paint program Tip You can paste the image into any Microsoft program including Microsoft Word and Outlook mail Paint generally provides a smaller file when zipped 3 In the Paint Edit menu choose Paste Do not stretch or re size the image as this will make it hard to read 4 In the Paint File menu choose Save Use the Save As dialog box to choose an appropriate directory ...

Page 53: ...on Customer Support The Thermo Electron Customer Support Group can be reached by telephone fax or e mail from 8 am to 5 pm EST To telephone 1 905 332 2000 voice 1 800 365 7587 voice toll free in Canada and United States To fax 1 905 332 1114 facsimile To e mail Customer Support services labautomation thermo com ...

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Page 55: ...l lockout 4 2 e mail notification 2 2 EMERGENCY STOP See E Stop environment conditions 1 10 error messages 2 2 2 6 capturing 5 5 E Stop button 1 6 1 12 2 2 chain 5 4 chain test 4 3 circuit 1 6 recovering from 2 7 reset 2 7 test 3 3 G GPIO expander 1 6 guarding 1 7 2 3 I instruments list of 1 2 L LAN 1 6 LAN hub system 1 7 layout of system 1 2 list of instruments 1 2 lockout air 4 2 electrical 4 2 ...

Page 56: ... 3 screen capture 5 6 serial expander 1 6 serial port 1 6 setup data validation 3 7 start POLARA 3 7 support before contacting Thermo Electron 5 3 system computer 1 6 system LAN hub 1 7 system layout 1 2 system offline 3 5 T telephone notification 2 2 U uninterruptible power supply 1 7 3 5 UPS 3 3 UPS See uninterruptible power supply W warnings and error messages 2 6 WinPREP system configuration 1...

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