Teledyne CombiFlash EZ Prep, User Manual

Page: 60 / 245

CombiFlash® Rf
+
User Manual
2-24
Use ELSD by Default - causes all runs to use the ELSD
detector unless disabled in the run requirement screen
[S
TARTING A RUN USING DEFAULT SETTINGS [6.4.1]
].
2.13.8 Prep HPLC Tab
The
DEFAULT COLUMN
allows selection of the most commonly
used Preparative HPLC column. This will be the method
loaded when the user first selects use of the Preparative HPLC
function during normal operation.
Many times this will be the
column mounted onto the system. The drop down list to select
the
DEFAULT COLUMN
is based on the columns already defined
by the user. To define a column, refer to the area below the
DEFAULT COLUMN
field.
To define a column:
1. Press N
EW
. This will open a dialog box allowing the new
column to be named. This name will be used to refer to the
column in normal usage. This name will also be printed on
the report.
2. Select the
MEDIA SIZE
. This data will be printed on the
chromatograms for future reference.
3. Enter a
COLUMN DIAMETER
. This information will be used
by the system to recommend an optimal flow rate to
achieve highest resolution for the column being defined.
4. Enter a
COLUMN LENGTH
. This is used to recommend a
separation length. This length is based on a gradient
segment approximately 12 column volumes in duration.
5. Select
DEFINE METHOD
. This allows a review of the method
defined by the system. This is an opportunity to edit the
recommended gradient shape and duration to better match
the expected chemistry. A higher flow rate can be selected
to balance resolution and throughput.
6. Selecting S
AVE
. This will save the column and allow its use
on the system.
7. If you are done defining columns, press the
OK
button.
If you have several columns that will be used on the system,
these can be defined at this time or at any later time.
• S
AVE
A
S
allows you create a new column definition
based on an existing column. This is done by selecting
the existing column in the C
OLUMN
N
AME
drop down