7. Quality Control
102
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
7.4.1.5 Detection Limit Bottom Fluorescein 384-well Plate
Pipette 100 µl of a 1 nM Fluorescein solution (diluted in 0.01 M NaOH) into the
wells of a Greiner 384-well plate (black, µClear, flat bottom) according to the plate
layout.
Material:
Greiner 384-well plate, flat bottom, µClear (black with transparent bottom)
100 µl pipette (8-channel) + tips
1 nM Fluorescein (diluted in 0.01 M NaOH)
0.01 M NaOH
Plate Layout:
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1
2
3
4
5
6
7
8
9
10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
A
F B F B F B
F
B
F
B
F B
B
C
F B F B F B
F
B
F
B
F B
D
E
F B F B F B
F
B
F
B
F B
F
G
F B F B F B
F
B
F
B
F B
H
I
F B F B F B
F
B
F
B
F B
J
K
F B F B F B
F
B
F
B
F B
L
M
F B F B F B
F
B
F
B
F B
N
O
F B F B F B
F
B
F
B
F B
P
(F….100 µl 1 nM Fluorescein; B…..100 µl 0.01 M NaOH = Blank)
Measurement Parameters:
Measurement mode:
Fluorescence Bottom
Excitation wavelength: 483 nm
Emission wavelength: 525 nm
Excitation bandwidth:
20 nm
Emission bandwidth:
20 nm
Gain:
Optimal
Read Mode:
Mode 2 (100 Hz)
Number of flashes:
12
Lag time:
0 µs
Integration time:
40 µs
Plate definition file:
GRE384fb.pdfx, whole plate