6. Operating the infinite® 200
2008-07
Instructions for Use for
infinite® 200
No. 30017581 Rev. No. 1.4
95
6.2.3
General Options
The following options may be taken independently from the particular
measurement technique.
Note
To keep temperature on a constant level and provide uniformity
across the plate, the plate must be placed in “incubation position”.
When the “heating” function is used during shaking,
the temperature may vary slightly.
Temperature Control
Some assays ask for an exact operating temperature. The
infinite
®
200
can set
up a specific temperature within a specific range, provide uniformity across the
plate, and keep temperature constant above ambient. The main cooling fans stop
ventilation.
Heating up the measurement chamber will take some time. Please check the
temperature control display. If not incubated externally, the microplate should be
left for equilibration before the measurement is started.
Temperature range: 5 °C above ambient to 42 °C.
Kinetic Measurements
i-Control allows a plate to be measured repeatedly in equidistant time intervals.
Fluorescence signal may significantly decrease over a longer period of time,
especially when using low volumes. Depending on the amount of evaporation, the
meniscus will shift to a lower position giving rise to slightly out of focus conditions.
Usually, wells in the corner evaporate faster, the next at the edges of the
microplate. When measuring fluorescence, decrease in signal may also result
from photo bleaching.
Microplate Shaking
infinite
®
200
provides two shaking modes: linear and orbital. The shaking
amplitude can be selected from 1 – 6 mm in steps of 0.5 mm. The frequency is a
function of the amplitude. The shaking duration is selectable from 1 – 999 s.
Multi Labeling
i-Control
provides a basic Multi Labeling capability. Up to four sets of instrument
parameters can be edited. The corresponding plate measurements will be
executed in the selected order. For example, when using more than one
fluorescent label, different filter combinations could be selected. A multi labeling
measurement can be set up by using a plate strip with/without a ‘part of the
plate’-strip and up to 10 measurement strips (absorbance fixed wavelength,
absorbance scanning, fluorescence intensity, fluorescence intensity scanning,
luminescence)
