5. Operating the Instrument
96
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
5.6
FP Measurements
5.6.1
Fluorescence Polarization
Fluorescence Polarization (FP, P) is defined by the following equation:
Equation 3:
where
I
and
I
⊥
equal the emission intensity of the polarized light parallel and
perpendicular to the plane of excitation, respectively. Polarization is a
dimensionless unit, generally expressed in mP units.
To start an FP measurement, the program strip must contain a valid
measurement
Blank range
and valid
G-Factor
settings.
5.6.2
Measurement Blank Range
Measurement blank reduction is performed automatically at each fluorescence
polarization measurement; the mean value of the respective blank wells will be
subtracted from each sample value (see 5.6.8).
In the
Measurement
group box, select the
Blank range
by clicking
Change
and
then selecting the wells filled with the measurement (sample) blank.
(
)
(
)
⊥
⊥
+
−
=
I
I
I
I
P
||
||
