TA Instruments Nano ITC 5300, Getting Started Manual

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Nano ITC Getting Started Guide Page 29
Figure 12 Side view of sample cell and access tube.
b Make sure that the reference needle is inserted into the reference cell access tube after filling (see
the figure below). The liquid should be just visible at the bottom of the conical overflow reservoir
when the cell is filled.
Figure 13 Sample (center) and reference (right) cell access tubes.
3 Load the 100 µL syringe with the titrant, taking care to remove any bubbles from the barrel of the syringe,
but leaving a small, 5 to 10 µL air gap between the plunger tip and the liquid in the barrel. Leaving an
air gap is a critical step needed to prevent signal distortion. Fill the syringe to a slight excess, 2 or
3 mm beyond the highest gradation of the barrel.
CAUTION: The signal can include overly large blank effects if there is no air gap to serve as a
cushion at the plunger tip
Conical overflow
reservoir - holds
excess liquid as
titrant is added
Fill level