Instrument Operating Manual
CyFlow® Cube 8 Rev-27_2015-07-02
Sysmex Partec GmbH
Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · [email protected] ·
www.sysmex-partec.com
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9.4
Technical Specifications
Note: Due to fast technological improvements, specifications herein are subject to
change. For details, please inquire information from your local supplier.
9.4.1
Example for Optical Standard Setup
Optical bench of a CyFlow
®
Cube with 2 lasers, UV-LED and 6 parameters.
The Parameters
FSC:
forward scatter
SSC:
side scatter
FL1:
green fluorescence
fluorescence origin: 488 nm laser (GFP)
FL2:
orange fluorescence
fluorescence origin: 488 nm laser (PE)
FL3:
blue fluorescence
fluorescence origin: UV-LED (DAPI)
FL4:
red fluorescence
fluorescence origin: 638 nm laser (APC)
If required by a specific application, the
optical standard setup can be optimized by
exchanging
preassembled
removable
mirror/filter blocks. This is a matter of
seconds and does not require any re-
adjustment.
The CyFlow
®
Cube flow cytometer can be
equipped with various light sources and up to
eight optical parameters. Depending on the
number
of
light
sources
and
optical
parameters different optical benches are
available.
Due to its modular concept the optical
configuration can be adopted to many
different clinical and scientific purposes.
Standard configurations are presented below:
Example:
This instrument is equipped with a
blue diode pumped solid-state Laser (20 mW) at
488 nm, a red laser diode (25 mW) at 638 nm
and a 365 nm UV-LED.
