SynOptics SYNBIOSIS aCOLyte3, User Manual, Page: 33 / 110

The Classification tab
To change the type of frame used to define the region for counting colonies:
1. Press the frame button to display the frame menu.
2. Choose the required option – the contents of the menu depend on the type of plate and
are described in the following sections:
l
Pour Plate batches , below
l
Spiral Plate batches , below.
Note You can change the type of frame in the Batch Designer or Measurement mode.
Pour Plate batches
You can choose Circular Frame or Rectangular Frame, or, to use a frame covering a single
sector only, choose 1/2, 1/4, 1/8 or 1/10 from the Partial Plate Frame submenu:
Partial plate frames can be useful when you have plates that are heavily loaded with bacteria,
or if there is some problem with a plate and you just want to read results from an unaffected
part. This means that you are more likely to want to choose a partial plate frame for an
individual plate while you are working in Measurement mode than for all the plates in a batch
when you are creating it in the Batch Designer.
Note You can also avoid counting in problematic areas of individual plates by placing Exclude
regions over them – see
Exclude regions , page 69.
Spiral Plate batches
You can choose Whole Frame, Annulus Frame or 2 Sector:
Notes As a general rule, if there has only been a small amount of colony growth, you should use one
of the Whole Frame options (use Annulus Frame if the spiral plater does not reach the center
of the plate; use Whole Frame if it does reach the center), otherwise, use 2 Sector.
If you choose 2 Sector and the number of colonies counted in the first sector falls below the
threshold under which all colonies are counted (see
Creating a completely new batch ,
page 18, or ), aCOLyte3 will count the whole frame.
aCOLyte3 User Manual 27