20
Write Protection (Off by default)
UNLOCKED P=500 JAN 18, 1997 2:30 PM
3 1 HEAT=525,PULL=90,VEL.= 80,TIME=250
Figure 3-6. Write protection default state.
3.3.8
MEMORY TEST < 8 >
Performs a non-destructive test of the RAM. Press RESET to reset the system after
performing this test. On newer versions of P-97 pullers, the Memory Test is no longer a valid
test.
3.4
Pulling Pipettes
3.4.1
Procedures
Prior to pulling a pipette for the first time, it is important to establish what HEAT value is
appropriate to melt your glass. Before executing a program for the first time, run the RAMP
TEST as previously described, and note the Ramp value reported. The HEAT settings in the
factory-installed programs are typically set at the Ramp value. If your Ramp test value
differs from the factory Ramp value, adjust the HEAT in your programs to your Ramp value.
Once you have adjusted the HEAT value relative to the Ramp value, pulling a pipette is very
straightforward. Try executing the factory installed programs with the sample glass to
become familiar with the pulling process.
1.
Load the glass into the puller as described previously in the FIRST TIME USE chapter.
2.
Press < 0 > followed by < ENTR > on the keypad to view Program 0.
3.
Inspect the parameter values displayed for Program 0. Program 0 should display the
factory-installed values listed on the enclosed program sheet. Adjust the HEAT setting to
your Ramp value.
4.
Press the < PULL > key on the keypad. The heating filament will turn on and the glass
should separate within 10 seconds. The display will then report the number of heating
cycles and the total time that the heat was on, similar to the following:
THE PROGRAM LOOPED 01 TIMES. THE LAST
LINE USED WAS 01, HEAT ON SEC. = 6.05
Figure 3-7. Pull Cycle Report.
5.
Loosen the clamping knobs and remove the pipettes from the puller bars.
Unless otherwise stated on your program sheet, Program 0 is factory pre-programmed to pull
a micropipette (tip diameter less than 0.1 mm) from 1.0mm x 0.5 mm borosilicate glass. It
will pull the pipette in one heating cycle or ‘loop’. The time reported is very useful for
developing programs and will be discussed in the Parameter Adjustment section of this
manual.
P-97 FLAMING/BROWN MICROPIPETTE PULLER OPERATION MANUAL – REV. 2.43 - DOM (20161118)
Summary of Contents for P-97
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