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3.2.8.1
Entering a New Program
After selecting or clearing a program area, the cursor will be blinking at the HEAT
parameter on Line 1. To familiarize yourself with program entry, enter the following sample
micropipette program:
HEAT PULL
VELOCITY
TIME
525 80 90 250
Press the series of numbers < 525 > to enter the HEAT value of 525. The cursor will
automatically tab over to the PULL parameter.
Enter < 80 > for PULL. The cursor will tab over to the VELOCITY parameter.
Enter < 90 > for VELOCITY. The cursor will remain there, blinking. Note that when less
than three digits are entered, the cursor will not automatically tab to the next field. You
must press < ENTR > to enter the value and tab to the next field.
Enter < 250 > for TIME. The cursor will tab to the next field, which is HEAT in Line 2.
3.2.8.2
Editing an Existing Program
To edit the value of a parameter(s) in a particular line, the cursor must be blinking in the
field you want to edit. If necessary, press < NEXT > or < LAST > to scroll to the line you
want to change. Press < ENTR > to tab the cursor to the field you want to change, and enter
the new value. In our example above, to change the value of VELOCITY in Line 1, you first
press < LAST > to scroll up to Line 1. Then press < ENTR > two times to tab over to the
VELOCITY field. Note that the values for HEAT and PULL remain unchanged. Enter a new
value (e.g., 90); if the value is two digits, you must press < ENTR > to enter the value. The
cursor will be positioned on Line 1 in the TIME field. To move to Line 2, press < ENTR > to
“tab” over to the HEAT field in Line 2.
The program values above are only given to demonstrate data entry procedures. Useful
program values to pull the pipette that you want will be discussed in the next chapter. In
general, inappropriate settings will only affect your ability to control the geometry of the
glass micropipette you are trying to fabricate. However, the heating element can be destroyed
by an excessive value for the HEAT parameter. Do not use HEAT settings greater than those
found in programs 0 and 1 until you have run the RAMP TEST described in the next section.
3.3
Software Control Functions
The P-97 has several unique software CONTROL FUNCTIONS that allow you to, among
other things, run SELF-TEST procedures, set up some of the PULL CONDITIONS and
WRITE-PROTECT your program. These control functions are accessed through the <CLR>
key. Pressing <CLR> gives you the following message:
DO YOU WISH TO CLEAR ALL VALUES FROM THE
PRESENT CYCLE TO THE END? NO=0 YES=1
Figure 3-5. Access to control functions.
Entering < 0 > will provide you with access to the following P-97 CONTROL FUNCTIONS:
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